Spectrum Measurement - PerkinElmer 200A Series User Manual

Fluorescence detector
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7. CHECK (Xe lamp energy check)
Energy level of the xenon lamp can be checked using the xenon lamp beam at 254 nm.
a. Press the GLP key.
b. The following display will be presented.
<GLP initial screen>
Select Xe CHECK.
Press numeric key 4 and then ENT key.
If the Xe lamp is turned off, the system will return to the 1st monitor screen without performing
an energy check.
c. The Xe lamp energy check screen appears and an energy check will be performed.
<Xe lamp energy check screen> (energy check is in progress)
d. The result of Xe lamp energy check will be displayed automatically.
<Xe lamp energy check result>
Press the ENT or ESC key.
If 5 minutes or more elapses without pressing ENT or ESC key, the system will return
automatically to the 1st monitor screen.
e. When the Xe lamp energy check is finished, the system returns to the 1st monitor screen.
When the temperature of the Xe lamp is high, the sealed gas pressure will fluctuate and the lamp may be
NOTE:
difficult to ignite. Before restarting the instrument or re-lighting the lamp, wait at least five minutes for
the lamp to cool down after turning it off.

Spectrum Measurement

Set the wavelength scan range (maximum and minimum values) for excitation (Ex) and emission (Em),
then measure and record the spectral data for each.
Carry out recording of spectral data with sample contained in the cell (sample not flowing, but
stationary in cell).
GLP
(LOGBOOK=1, KEY LOCK=2,
4
WL CHECK=3, Xe CHECK=4)
CHECKING LAMP ENERGY
Xe ENERGY
1200
Operation
91
(Xe)

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