Troubleshooting - PerkinElmer 200A Series User Manual

Fluorescence detector
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158 Series 200a Fl User's Guide

Troubleshooting

Symptom
Cause
Self-diagnosis
The power cord plug
(initialization) is not
is not securely
performed when the
plugged in.
POWER switch is
turned on.
The fuse is blown.
The light source
cover is not properly
attached. (The cover
activates a safety
interlock switch.)
The keyboard does
The instrument is not
not work.
ready for operation.
The A/Z key does not
The display does not
work.
present the 1st
monitor screen (FL
indication).
The time program
The instrument is not
does not start.
ready to operate.
Excessive noise
The flow cell is
contaminated.
The mobile phase is
not sufficiently
degassed.
The mobile phase
contains impurities.
The gain level of the
recorder is too high.
The pump is faulty.
Judgment/Check
Remedy
Visual check
Plug in the power cord
securely.
Check the fuse for
Replace the fuse with a new
continuity.
one. (Refer to Section 6.3.)
Visual check
Retighten the retaining
screws of the light source
cover.
Press the ESC key.
Check the monitor
screen.
The READY status will be
presented.
Check the monitor
Return to the 1st monitor
screen.
screen (FL indication).
(Refer to Section 4.1.)
Check the monitor
Press the ESC key. The
screen.
READY status will be
presented.
Visual check
Clean the flow cell. (Refer
to Section 5.5.)
Spike noise occurs.
Degas the mobile phase
thoroughly.
Check the mobile
Purify the mobile phase, or
phase.
replace it with mobile phase
of a higher quality.
Check the gain level
Adjust the gain level of the
of the recorder.
recorder.
Check the pump.
Check to ensure the
operation of the
pump is smooth.
Check for noise when
the pump is stopped.

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Troubleshooting

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