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Principle of Operation
Plane mirror
Xenon
lamp
Xenon lamp
starter
Xenon lamp
ballast
The beam from the xenon lamp is converged and strikes the excitation monochromator that disperses the
radiation via the large-aperture concave grating. Only radiation of the specified wavelength is allowed to
pass through the exit slit. After the exit slit, the excitation radiation strikes the sample.
The fluorescence (emitted radiation) enters the emission monochromator, where it is dispersed by the
monochromator. The selected radiation strikes the measuring detector (photomultiplier).
The monitor detector (photodiode) measures the intensity of the excitation beam as a function of time.
Excitation
monochromator
Measuring
detector
(Photomultiplier)
Sample
fluorescence
amplifier
Fig.3-12 Functional Block Diagram
Sample
(Flow cell)
Emission
monochromator
(Fluorescence
monochromator)
Photomultiplier
high voltage
circuit
Operational
circuit
Recorder
or integrator
Electrical connection
Optical path
System Description
Diffusion plate
Monitor detector
(Photodiode)
Monitor detector
amplifier
51
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Table of Contents
Troubleshooting
