PerkinElmer Wallac 1420 User Manual
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PerkinElmer Wallac 1420 User Manual

PerkinElmer Wallac 1420 User Manual

Software version 3.0
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Enlarged version
1420-922-07
March 2003
User manual
1420
Software version 3.00
PerkinElmer Life and Analytical Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.
Tel: 358-2-2678111. Fax: 358-2-2678357. Website: www.perkinelmer.com/lifesciences

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Summary of Contents for PerkinElmer Wallac 1420

  • Page 1 1420-922-07 March 2003 User manual 1420 Software version 3.00 PerkinElmer Life and Analytical Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland. Tel: 358-2-2678111. Fax: 358-2-2678357. Website: www.perkinelmer.com/lifesciences...

  • Page 3: Table Of Contents

    Enhanced Security mode ....................12 Software installation ......................13 Operation overview ......................14 Operating Wallac 1420....................... 15 Starting up Wallac 1420 ....................17 Without enhanced security mode.................. 17 With enhanced security mode..................17 Instrument control......................18 Live display ........................19 Temperature........................
  • Page 4 Contents Down button ......................45 Up button ........................45 Delete button......................45 Dispense button......................45 Delay button......................46 Shake button ......................46 Label button ......................46 Kinetics button ......................47 Scanning button ......................47 Injector position during measurement............... 47 Plate ..........................48 Plate repeat parameters ....................
  • Page 5 Fluorometry ........................75 Protocol name ......................75 CW-Lamp energy ..................... 76 CW-Lamp Control Mode..................76 CW-Lamp filters....................... 76 Excitation aperture (Wallac 1420-040/041/042/043 only) ........76 Emission filters ......................76 Emission aperture ..................... 77 Counter position ....................... 77 Counting time ......................77 Second measurement parameters................
  • Page 6 Protocol name ......................80 Emission filters ......................80 Emission aperture...................... 81 Counting time......................81 Second Measurement ....................81 Fluorescence polarization (Wallac 1420-040/041/042/043 only)......... 82 Protocol name ......................82 CW-Lamp energy...................... 83 CW-Lamp filter......................83 Polarizer aperture ...................... 83 Emission filter ......................83 Emission aperture......................
  • Page 7 Contents Filters ..........................90 Emission filters ......................91 CW-Lamp filters......................92 Filter slides ........................93 Emission filter slide ....................94 Physically changing the emission filter slide............95 CW-Lamp filter wheel ....................96 Physically changing the CW-Lamp filter wheel ............97 Changing the CW-Lamp...................
  • Page 8 Appendix 1: Connecting MultiCalc to Wallac 1420............139 Introduction......................... 139 Wallac 1420 MultiCalc assay protocols..............139 Installation of Wallac 1420 output option in MultiCalc ..........140 MultiCalc assay protocols................... 140 Usage of MultiCalc ..................... 140 Appendix 2: Plate types for stackers................141 Wallac ........................
  • Page 9 Audit Trail ........................158 Archive ........................160 Security Settings ......................161 Index ..........................165 Trademarks DELFIA is a registered trademark and LANCE is a trademark of PerkinElmer, Inc. Windows and Excel are registered trademarks of Microsoft Corp. in the U.S. and other countries.

  • Page 11: Introduction

    Introduction...

  • Page 13: System Overview

    Introduction System overview Note: This manual describes all the features of Wallac 1420. Some versions of the instrument do not have one or more of these features. You can ignore the parts of this manual not relevant to the version of Wallac 1420 you have.

  • Page 14: Enhanced Security Mode

    • audit trails of user actions This manual and the electronic help will tell you how to use Wallac 1420 with or without the Enhanced Security mode. All differences in use are noted and explained. Note: use of the Enhanced Security mode does not alone ensure compliance with 21 CFR Part 11.

  • Page 15: Software Installation

    Introduction Software installation Note: the following instructions assume that the counter, PC and printer have been connected up and switched on (see the installation instructions in the Instrument manual). If Enhanced Security mode is used then installation can only be done by a person with System and Windows administrator rights.

  • Page 16: Operation Overview

    Introduction Operation overview Parameters must first be set using the protocol editor. This is activated by selecting a protocol in the Explorer. A sample plate can then be loaded and measured using the protocol set for it. Other parameters affecting the whole system e.g. labels, filters etc. can be set in the Tools menu.

  • Page 17: Operating Wallac 1420

    Operating Wallac 1420...

  • Page 19: Starting Up Wallac 1420

    Operating Wallac 1420 Starting up Wallac 1420 Without enhanced security mode When you boot the system the following window appears: With enhanced security mode When the Enhanced Security mode is installed the login dialogue appears after you have started Wallac 1420.

  • Page 20: Instrument Control

    Wallac 1420. Clicking the Change password opens the Change Password dialogue where you can define a new password. After a successful login Wallac 1420 can be used according to the permissions granted to the logged on user. Instrument control The main window has three tabs: Instrument Control, Live Display and Temperature.

  • Page 21: Live Display

    When the workstation is used without an instrument (i.e. running in demo mode), the status bar includes the text “DEMO”. When Wallac 1420 workstation is connected to an instrument (running in normal mode), the demo mode indicator is blank. You can switch between normal and demo modes using the Options selection in the Tools menu.
  • Page 22 Operating Wallac 1420 You can choose the result range you want used to determine the colour code of the wells. The closer a result is to the upper limit of the range, the more red it will appear. The closer to the lower limit it is, the more blue it will appear.

  • Page 23: Temperature

    Operating Wallac 1420 Temperature In the Temperature tab of the main window you select plate heating and the target temperature. The latter can be set with 0.1 C precision within the interval 15 – 45 C. The minimum temperature is 15...

  • Page 24: Barcode Options

    The figure shows a typical 96-well plate and how it is to be orientated when loading it either directly into Wallac 1420 or into an input stacker. The A1 position must be in the left corner furthest from you i.e. the side that enters the counter first. If a barcode is used, it should also be on the side that enters the counter first.

  • Page 25: Starting Operation With The Start Button

    Operating Wallac 1420 Starting operation with the Start button 1. Open the loading position lid. 2. Load your first plate and close the lid. See the picture on page 20 for correct plate orientation. Load first plate...
  • Page 26 Operating Wallac 1420 3. Select the protocol you want to use by scrolling through the list of active protocols. 4. Use the mouse to click the Start button on the user interface You can follow the measurement process by selecting the Live display .
  • Page 27 Operating Wallac 1420 5. When the plate has been measured, remove it and load the next plate. 1. Remove the measured plate 2. Load the next plate 3. Press the button briefly 6. Briefly press the button on the counter, this will be blinking if there are more plates to be loaded for this assay.

  • Page 28: Starting Operation With The Start Wizard

    Operating Wallac 1420 Starting operation with the Start Wizard In the enhanced security mode the Start Wizard is enabled only for users who belong to the Operator user group. An alternative way to start counting is to use the Start Wizard. This allows you to define how many plates you are going to use and to give the positions of samples.
  • Page 29 Operating Wallac 1420 The steps are: Introduction to the wizard. (You can select the introduction not to appear next time if you want). Protocol selection - click the protocol you want to use for measurement. Definition of the sample names and number of plates. See Samples tab on page 39 for a description of the principles involved in sample definition.

  • Page 30: Starting Operation And Using A Protocol Number Barcode

    Operating Wallac 1420 Starting operation and using a protocol number barcode 1. When you load a plate, the button will be blinking. See the picture on page 20 for correct plate orientation. Load the first plate Light is blinking 2. Press the button briefly. Operation will start. The button light will be lit without blinking.

  • Page 31: Stacker Operation

    Operating Wallac 1420 Stacker operation A stacker is able to hold up to 20 (or 40 in the larger version) microplates. No lids, loose frames or very loose strips are acceptable. See Appendix 2 for a list of plates that fit in the stackers.
  • Page 32 Wallac 1420 because you may jam the conveyor. If the button on Wallac 1420 is blinking, it means that the assay has not finished and you must remove the empty input stacker, load an empty output stacker and then an input stacker which has the remaining plates of the assay (plus plates for other assays if you want).

  • Page 33: Starting Operation And Using A Protocol Number Barcode

    Operating Wallac 1420 Starting operation and using a protocol number barcode Caution Do not put your fingers into the sample loading area. In the stacker model the loading area is uncovered when the stackers are not in place so you must avoid the danger of getting your fingers trapped by the plate lift mechanism.

  • Page 34: Ending Operation

    Operating Wallac 1420 Ending operation Stop button Clicking the Stop button in the workstation interrupts the measuring process immediately. The plate being measured is brought out to the loading position. You cannot resume measurement after clicking Stop but must begin the assay from the beginning if required.

  • Page 35: Button On Wallac 1420

    Light on the button The following table shows you what the light on the button will be like depending on what is happening with Wallac 1420 and whether or not the protocol barcode option is used. Condition Barcode none or plate...
  • Page 36 Operating Wallac 1420...

  • Page 37: Protocol Editing

    Protocol editing...

  • Page 39: Explorer

    Protocol editing Explorer In the enhanced security mode Explorer is enabled only for users who belong to the Editor user group. Through the Explorer you can access the Protocol Editor and the Result Viewer. Click the Explorer icon to open the Explorer. The Explorer will open.

  • Page 40: Explorer Folders

    Protocol editing Explorer folders In the Wallac folder there are, as a default, folders named according to the technologies installed. In each of these folders there are protocols using the appropriate technology. On the same level as the Wallac folder there are two other folders: Users and Projects. To open a folder, click on it and then select Open from the File menu.

  • Page 41: Protocol Selection

    Protocol editing Protocol selection You can: Open , copy , paste , start or create a protocol, delete a user made protocol (plus its associated results). In the enhanced security mode you have to belong to the Operator user group to start a protocol. To open an existing protocol you can do one of the following: a) Move the mouse cursor onto it and then click the left mouse button to select it, then click the File menu and click Open .

  • Page 42: Protocol Editor

    Protocol editing Protocol editor Note: changes to protocols should only be made by authorized persons. The protocol editor window contains seven tabs: Samples - shows a plate map to allow you to edit wells and plates ID - you can identify the protocol Measurement - you can select the labels used for measurement Plate - you can select the plate type and other parameters Outputs - you can define the output...

  • Page 43: Samples

    Protocol editing Samples Selecting this tab causes a plate map to be displayed to allow you to edit samples. Buttons allow you to select the first plate, previous plate, next plate or last plate . You can also add a new plate, delete the currently selected plate or duplicate a plate. An added plate has all the positions marked as Measured and will become the last plate in the sequence.
  • Page 44 When you have selected the wells and released the left mouse button, a drop down menu of sample types will appear. These will be the types defined in the Wallac 1420 Manager Tools menu item Miscellaneous settings . By selecting one of these types you specify what...
  • Page 45 Protocol editing This tab allows you to select the protocol number and name. The number can be selected from a drop down list of available numbers. The protocol name has to be typed in. In the case of a factory pre-set protocol you cannot change the number. Notes or additional comments can be connected with the protocol.

  • Page 46: Measurement

    Protocol editing Measurement The measurement tab specifies the measurement sequence to be used in the protocol. Note: If the protocol being edited is pre-set, the measurement sequence cannot be changed. Measurement mode This field allows you to specify on what unit the complete measurement sequence should be performed before moving on to the next similar unit.

  • Page 47: Measurement Operations

    Protocol editing can be used for slow kinetics by making repeated counting of the plate with delays in between. Measurement operations This field lists the operations that have been specified to be used in the assay. The operations are listed in the order they will be performed. If you select an item in the list and then click the right mouse button, a menu will appear to allow you to Copy , Delete or see the Properties of the selected item.

  • Page 48: Delay Button

    Protocol editing the number of replicates (from 1 to 3) the injection mode . You can either aspirate and then dispense the same amount (aspVol=dispVol) or you can fill the whole syringe and then dispense several times (aspVol=syringeVol). The former is more accurate but the latter is faster. At the bottom of the dialogue is a check box “Perform on first plate repeat only”.

  • Page 49: Kinetics Button

    Protocol editing Kinetics button Clicking this causes a dialogue to appear that lets you specify the parameters for a kinetic measurement. You can give the number of repeat measurements (up to 100) and the delay between each measurement (0 to 600 s). You need to also select the label by clicking the label button and then selecting from the label dialogue.

  • Page 50: Plate

    Protocol editing Plate Plate repeat parameters The number of times each plate is to be measured (1-99). If this is more than 1 then you must give the time between the end of one measurement and the beginning of the next (0- 3600 secs.).

  • Page 51: Plate Type

    Make sure you select the plate type before defining the plate map to avoid losing your settings. Plate types are read from the Wallac 1420 database when the Protocol Editor is started so it will not recognize plate types added while the Protocol Editor is open.

  • Page 52: Outputs

    Protocol editing Outputs The Outputs tab in the Protocol editor allows you to select where results will be sent at the end of a run. This can be to a printer and/or file. Click the check box of the output device(s) you want.
  • Page 53 Protocol editing If you have selected File output you must specify the file type from the drop down menu of possible types. The types you can save files in are Excel 5 or later versions, Tab delimited text or MultiCalc. If the Include in output list box is checked, then it specifies which optional parts of the assay run information should be included in the file saved when the measurement is completed.

  • Page 54: Events

    Protocol editing Events This tab allows you to give a command that will be executed when a protocol has been run. The keyword should be in triangular brackets < > exactly as the examples given in the tab show. These commands are then automatically replaced by the appropriate name or number etc.

  • Page 55: Keywords

    Protocol editing Keywords The following keywords can be used for file names in the protocol editor Outputs tab and in Assay end system command in the Events tab: <ProtocolName>, the name of the protocol. Specified in the ID tab in the protocol name edit box.

  • Page 56: General

    The user names used come from the identifier given when Windows was booted. Pre-set protocols are created in the factory. In the enhanced security mode the first user name is the system user name and the second is the Wallac 1420 user name. User names are separated with a slash.

  • Page 57: File Menu

    Protocol editing File menu The protocol editor file menu contains the following items of which Save and Print have corresponding buttons. Save When you select this item or click the button the current protocol information is saved. Print When you select this item or click the button a dialogue opens up for you to select the printer type and other details of the printout.

  • Page 58: Tools Menu

    Protocol editing Tools menu The Tools command line menu has one item Options . When you click this a dialogue with a check box appears. If you do not check it, then, whenever you click the Start icon, measurement of the selected protocol will begin directly. If you do check it, then, whenever you click the Start icon, the Start Wizard will be used to start the measurement of the selected protocol.

  • Page 59: Result Viewing

    Result viewing...

  • Page 61: Latest Results

    Alternatively, once results have been received and stored in the computer, you can view them by means of the Wallac 1420 Explorer. See the picture on the next page. The protocol connected to the latest results will be selected automatically, but you can select any protocol that was used to make the measurements that you want to view.
  • Page 62 Result viewing down the left mouse button and move the column division in the direction you choose. The information in a column is complete if no “..” are shown at the end. Click on the result file and then click the Open icon. Note: these results are in the form of raw numerical data The Result Viewer gives details of the actual measurement.

  • Page 63: List

    Result viewing List The following information appears: Plate ID, Number of repeats, Well number and Sample type abbreviation (e.g. M= Measured). In addition there are pairs of columns. The number of pairs depends on what you have set in protocol editing parameters. The first column of each pair is the measurement time and the other the result value.

  • Page 64: Plate Map

    Result viewing Plate map This plate map shows you the results arranged from top to bottom in the order they appeared in the list from left to right. See List format for the alternative way of viewing results. The cells in the two first rows displayed include information about the currently displayed plate.

  • Page 65: Protocol

    Result viewing Barcode: Barcode of the plate if used, if not used it displays the text ‘N/A’. The cells displayed in the rows directly above each result plate gives information directly relating to the label or result type displayed in the plate grid below it. The information included is: Name of the label or result type displayed in the result plate Unit for the results from the result plate...

  • Page 66: Notes

    Result viewing Notes This tab allows you to see any notes that are connected with the results being viewed. Error Error gives you information about the general error situation and specific information about any errors that occurred for each measurement type. Print Clicking this icon leads to an extended print dialogue which in addition to the normal print options gives you a choice of printing selected sheets or the entire workbook .

  • Page 67: Tools Menu

    Tools menu...

  • Page 69: Tools Menu

    Tools menu Tools menu Note: These operations can affect the whole system and should only be undertaken by someone qualified to do them. There are up to fifteen items in this menu (depending on the options installed): Explorer - for protocol editing and result viewing, see pages 35 and 57. Start Wizard - for starting when you first need to define plates and wells, see page 24.

  • Page 70: User Level

    Tools menu User level Note: in the enhanced security mode extra menu items appear. The User level in the Tools menu allows you to select the user level: Routine , Advanced or Service . If the User Level is selected to be Routine , this will automatically disable any system operations.

  • Page 71: Labels

    Tools menu Labels In the enhanced security mode this tool is enabled only for users who belong to the Editor user group. In this window there are tabs corresponding to the measurement technologies (labels) in the instrument. Each tab includes the names of the currently defined labels. Names of user defined labels can be freely defined by the user but names of factory pre-set labels cannot be changed.
  • Page 72 Tools menu Remove - (select a user created item to activate this). Remove the selected item from the list. Preset items and labels used in protocols cannot be removed. This command requires confirmation because you cannot undo Remove. Properties - (select an item to activate this). View the properties of the selected item. If the item is user defined then you can edit the properties, but if it is a factory pre-set, you cannot edit them.

  • Page 73: Time-Resolved Fluorometry

    Tools menu Time-resolved fluorometry Note: to be enabled this requires the TR-FIA option to be installed. In time-resolved fluorometry a flash lamp is used to illuminate the sample, different lanthanides are used as labels. The picture shows the parameters that can be set. Protocol name This is the name by which the protocol is identified.

  • Page 74: Flash Energy Area

    Tools menu Flash Energy Area There are two options for this parameter “Low” and “High”. The energy of the flash lamp used is dependent on the discharge capacitor value. The “Low” Flash Energy Area selects a basic capacitor of 10 nF. The “High” Flash Energy Area selects a second 10 nF capacitor in the lamp’s discharge circuit thus doubling the energy.

  • Page 75: Note

    Tools menu Note Each flash contributes to the total excitation energy. Flashes keep on occurring until the required total excitation energy is achieved. However, because the energy comes in discrete flashes the last flash will cause the total excitation energy to exceed the required amount by a maximum of not more than the energy in one flash.

  • Page 76: Counting Parameters

    Tools menu Counting parameters These determine the time factors involved in the measurement process, all times are in microseconds: Counting delay - the time after the excitation pulse at which counting of the emission signal begins Counting window - the time period within which counting occurs Counting cycle - the time between excitation pulses within a measurement.

  • Page 77: Fluorometry

    Tools menu Fluorometry Fluorometry - light of a particular wavelength is selected with a filter from the spectrum of a continuous wave lamp and used to excite the fluorochrome in the sample. The parameters that can be set for fluorometry are the following: Protocol name This is the name by which the protocol is identified.

  • Page 78: Cw-Lamp Energy

    F is for fluorometry. After the letter comes the wavelength. Excitation aperture (Wallac 1420-040/041/042/043 only) There are three sizes of excitation aperture controlling the size of the incident light beam. You can select with the option buttons Small, Normal or Large. Normal is the default.

  • Page 79: Emission Aperture

    Tools menu Emission aperture In front of the photomultiplier tube there is a 4-position aperture slide, which has three different apertures and a shutter position. The normal aperture is circular with a diameter of about 4 mm and it is used for factory set labels.

  • Page 80: Photometry

    Tools menu Photometry A reference measurement is made before the plate is moved to the measurement position. This enables the absorbance to be calculated. The parameters that can be set for photometry are the following: Protocol name This is the name by which the protocol is identified. A password can be associated with it to prevent parameters being changed by an unauthorized person.

  • Page 81: Cw-Lamp Filters

    P is for photometry. After the letter comes the wavelength. Excitation aperture (Wallac 1420-040/041 only) There are three sizes of excitation aperture controlling the size of the incident light beam. You can select with the option buttons Small, Normal or Large. Normal is the default.

  • Page 82: Luminometry

    Tools menu Luminometry Luminescence in the sample is detected. The parameters that can be set for luminometry are show in the picture and described below. Protocol name This is the name by which the protocol is identified. A password can be associated with it to prevent parameters being changed by an unauthorized person.

  • Page 83: Emission Aperture

    Tools menu Emission aperture In front of the photomultiplier tube there is a 4-position aperture slide, which has three different apertures and a shutter position. The normal aperture is circular with a diameter of about 4 mm and it is used for factory set labels.

  • Page 84: Fluorescence Polarization (Wallac 1420-040/041/042/043 Only)

    Tools menu Fluorescence polarization (Wallac 1420-040/041/042/043 only) Fluorescence polarization - light of a particular wavelength is selected with a filter from the spectrum of a continuous wave lamp. This light is then polarized and used to excite the fluorochrome in the sample. The emission light is then viewed through two polarizers, one parallel to the incident polarization (S-plane) and one perpendicular to it (P-plane).

  • Page 85: Cw-Lamp Energy

    Tools menu CW-Lamp energy In fluorescence polarization constant voltage mode is used and the light source is driven with high power. The software corrects for changes in energy of the CW-lamp to ensure that results are calculated for a constant excitation energy. For fluorescence polarization you should always use maximum power, 65535 .

  • Page 86: G Factor

    Tools menu G factor In fluorescence polarization assays a G-factor is used to correct for the bias in polarization values caused by differences in instruments and assay conditions. To calculate the G-factor prepare buffer and 1 nM fluorescein samples (replicates should be at least 24). The formula used is: −...

  • Page 87: Lance

    Tools menu LANCE Note: this requires the LANCE option to be installed. In LANCE homogenous time-resolved fluorescence a flash lamp is used to illuminate the sample. In the most common form of LANCE this excites the donor molecule which, after a delay, transfers the energy to the accepter molecule which then emits light.

  • Page 88: Flash Energy Area

    Tools menu Flash Energy Area There are two options for this parameter “Low” and “High”. The energy of the flash lamp used is dependent on the discharge capacitor value. The “Low” Flash Energy Area selects a basic capacitor of 10 nF. The “High” Flash Energy Area selects a second 10 nF capacitor in the lamp’s discharge circuit thus doubling the energy.

  • Page 89: Note

    Tools menu Note Each flash contributes to the total excitation energy. Flashes keep on occurring until the required total excitation energy is achieved. However, because the energy comes in discrete flashes the last flash will cause the total excitation energy to exceed the required amount by a maximum of not more than the energy in one flash.

  • Page 90: Counting Parameters

    Tools menu Counting parameters These determine the time factors involved in the measurement process, all times are in microseconds: Counting delay - the time after the excitation pulse at which counting of the emission signal begins Counting window - the time period within which counting occurs Counting cycle - the time between excitation pulses within a measurement.

  • Page 91: Lance Label Properties

    Tools menu LANCE Label Properties • LANCE 615 : 100 flashes, window 50/100/1000, 615nm. This label is for fast decay Eu-chelates (for example Eu-W1024) in a TR-FRET assays. • • LANCE 665: 100 flashes, window 50/100/1000, 665nm. This label is for fast decay Eu-chelates (for example Eu-W1024) in a TR-FRET assays.

  • Page 92: Filters

    Tools menu Filters In the enhanced security mode this tool is enabled only for users who belong to the Editor user group. In this window there are three tabs: "Emission Filters", "CW-Lamp Filters" (i.e. continuous wave lamp excitation filters) and "Filter Slides" (including filter wheels). "Filter Slides" allows you to specify the slides for emission and CW-lamp filters.

  • Page 93: Emission Filters

    Tools menu Emission filters If you select properties of an emission filter or you want to define the properties of a new filter, a further dialogue appears where the name of the filter and the technology for its uses can be defined. There is also a section where you can add additional information that you can connect with the filter properties.

  • Page 94: Cw-Lamp Filters

    Tools menu CW-Lamp filters If you select properties of a CW-lamp filter or you want to define the properties of a new excitation filter, a further dialogue appears where the name of the filter and the technology for its use can be defined. There is also a section where you can add additional information that you can connect with the filter properties.

  • Page 95: Filter Slides

    Tools menu Filter slides There are two types of slide: Emission filter and CW-lamp excitation filter. The former is a strip and the latter a wheel. The instrument is loaded with slide A in each case but an empty slide B is also available for user filters as described below.

  • Page 96: Emission Filter Slide

    Tools menu Emission filter slide There are 8 positions in emission filter slides A and B. The physical dimensions of the filter slots are: diameter 25.5 mm depth 11 mm clear aperture 22 mm. All standard filters with diameter of 25.4 mm (1 inch) and thickness of 10 mm fit in the emission filter slides.

  • Page 97: Physically Changing The Emission Filter Slide

    Tools menu User-defined filters can be named freely but there is only space for five letters to be shown in the figure of the filter position. The full name will be shown if you move the mouse cursor onto the slot where the filter is. Physically changing the emission filter slide Click the Eject slide button on the Emission filter slide dialogue.

  • Page 98: Cw-Lamp Filter Wheel

    Tools menu CW-Lamp filter wheel Excitation filters fit into a wheel positioned in front of the CW lamp. There are eight positions on this wheel and two are reserved by default for fluorometry and three for photometry. The remaining positions can have filters added by the user. Note: default filters can be removed and replaced by user filters.

  • Page 99: Physically Changing The Cw-Lamp Filter Wheel

    Tools menu Physically changing the CW-Lamp filter wheel If you want to change a filter in the CW-Lamp filter wheel or you want to install wheel B then you must open the cover and lift out the filter wheel as shown in the picture. Either change the filters you want or take a new filter wheel B.

  • Page 100: Changing Filters In The Software

    Tools menu Changing filters in the software Note: if you change a filter in the software you must also change it physically in the filter slide or wheel. When you select Properties, a picture of the selected slide appears with the filters that are loaded in it marked in red or black as shown previously.

  • Page 101: Filter Properties

    Tools menu Filter properties The filters used in the instrument have the properties shown in the table below. The following abbreviations are used: CWL = Centre wavelength, BW = Bandwidth (Full Width Half Maximum), Tpk = Maximum transmittance at the peak wavelength (%) Filter type Name Centre...

  • Page 102: Eusm Dual Label Normalization

    To begin this procedure select EuSm Dual label normalization from the Wallac 1420 Manager Tools menu. This will activate a wizard that will guide you through the normalization procedure.

  • Page 103: Preparing The Plate

    Tools menu Preparing the plate The wizard explains what samples you should put in the plate and where you should put them. Plates must have 3 or more strips, (maximum of 8), with 1 - 12 wells per strip. The strips must be horizontal e.g.

  • Page 104: Measurement Status Page

    Tools menu Strip A should be full of blank samples, Strip B should be empty and Strip C should be full of europium samples. Measurement status page This is shown during the actual normalization measurement and it gives you feedback about the measurement.

  • Page 105: Normalization Confirmation Page

    Tools menu Normalization confirmation page This appears after normalization has been successfully completed. It shows the measured normalization values and requires you to confirm that you accept these values before they are stored in the database. The two values produced by the normalization are the background and the europium contribution to the samarium window.

  • Page 106: Lance Normalization

    LANCE measurements: background from the plate, crosstalk of the donor signal into the acceptor window, and quenching. The final result is normalized to the maximum donor intensity. To begin this procedure select LANCE normalization from the Wallac 1420 Manager Tools menu. This will activate a wizard that will guide you through the normalization procedure.

  • Page 107: Protocol Selection

    Tools menu Protocol selection You must select from the list of LANCE protocols the one you want to do the normalization for. Highlight the one you want and then click the Next button.

  • Page 108: Preparing The Plate

    Tools menu Preparing the plate The wizard explains what samples you should put in the plate and where you should put them. The strip to be used must be horizontal e.g. A1 to A12 etc. The plate used is the one defined in the protocol you previously selected.

  • Page 109: Measurement Status Page

    Tools menu Measurement status page This is shown during the actual normalization measurement and it gives you feedback about the measurement. The measurement status pane shows the current status of the measurement. The progress of the measurement is shown by the progress bar.

  • Page 110: Measurement Warning Page

    Tools menu The Next button becomes available when the measurement has been completed. If the measurement was completed without any warnings the window shows the text "Assay is ready". Click Next to move to the Normalization confirmation page otherwise the wizard moves to the Measurement warning page.

  • Page 111: Normalization Confirmation Page

    Tools menu Normalization confirmation page This is the last page of the LANCE normalization wizard and appears after normalization has been successfully completed. It shows you the measured normalization values and requires you to confirm that you accept these values before they are stored in the database. The values obtained are the blank measurement in the acceptor window, crosstalk, i.e.

  • Page 112: Using Lance Normalization Samples In The Assay

    Tools menu Using LANCE normalization samples in the assay Instead of running the LANCE normalization wizard and saving values that can be used for any subsequent LANCE assay with that particular protocol, you can include LANCE normalization samples on the plate as part of the assay samples and thus obtain corrected values for that particular plate.
  • Page 113 Tools menu When you have defined the plate and saved it, you can run the assay in the normal way. When the LANCE samples are detected on the plate, they will automatically be used to make the necessary corrections for this particular LANCE measurement. They will overrule any values that may have be stored in the instrument memory from a normalization with the LANCE wizard.

  • Page 114: Plate Dimension Wizard

    Tools menu Plate dimension wizard In the enhanced security mode this tool is enabled only for users who belong to the Editor user group. A description is given here of how the Plate dimension wizard can be used to fine-tune the plate dimensions.

  • Page 115: Plate Selection

    Tools menu Plate selection Note: This step requires that there is at least one user defined plate. Select the plate whose dimensions you want to fine-tune. Remember that this should be the plate type with the name you chose specifically for the technology to be used. Note that you must click in the Plate name field to select the plate.

  • Page 116: Preparing The Plate

    Tools menu Preparing the plate Prepare the plate by dispensing appropriately labelled sample to each of the four corner wells of the plate. All the other wells should be empty. Specify the area of the well to be scanned. The example shows the default value for a 1536-well plate.

  • Page 117: Define The Centre Point Of The Corner Wells

    Tools menu When the plate has been prepared, load it and click Next . After scanning is completed the Next button will be activated. Click it to continue. Define the centre point of the corner wells...
  • Page 118 Tools menu The window that appears allows you to define the centre point of each of the four corner wells. Each tab shows one of the four wells with a plot of the intensity of the light from the well. This is colour coded with red showing the highest intensity.

  • Page 119: Miscellaneous Settings

    Tools menu Miscellaneous settings In the enhanced security mode this tool is enabled only for users who belong to the Editor user group. In the dialogue that appears when you click this item in the Tools menu, there are three tabs for: Plate types the available plate types are listed including a number of default types.
  • Page 120 Tools menu Add - add a new item to the list available e.g. a new plate type. Give the name and edit the properties. It will appear with a new icon showing it is different from a default item. Copy - (select an item to activate this). Make a copy of an item. It will appear with a new icon showing it is different from a default item, and the name will be preceded by the words “Copy of”.

  • Page 121: Plate Type

    Tools menu Plate type When you click the Plate types tab the available plate types are listed. This list includes a number of default types. To define a new plate click Add . You can also click on an existing plate type and then click Copy to save the plate type with a new name.

  • Page 122: Number Of Well Rows

    Tools menu Number of well rows This is the number of wells in a row. The product of this with the number of well columns gives you the number of wells in the plate. Number of well columns This is the number of wells in a column. The product of this with the number of well rows gives you the number of wells in the plate.

  • Page 123: Options

    Tools menu Options This can only be accessed if you have Advanced level selected. There can be four tabs but only three show on Advanced level , the fourth requires Service level . In the enhanced security mode this can be accessed if you belong to the Service user group. All the four tabs plus the Automatic logout tab are shown.
  • Page 124 Tools menu If you want the start to be always by means of the Start Wizard, check this box. This means that when you click Start the Start Wizard will be activated. This is useful if e.g. you need to define the number of plates before each measurement. If you just want to run the software as a demo without having the counter connected, check the “Run Workstation in demo mode”...
  • Page 125 Tools menu external software can then process these files as from the same assay because each will have been produced by the same protocol. If you are not using stackers, the instrument waits until the button on the instrument is pressed and then begins measuring the current plate with the previously used protocol.

  • Page 126: Instrument Hardware

    Tools menu Instrument hardware This tab can only be accessed if Advanced level has been selected in User Level. In the enhanced security mode this tab can always be accessed. It shows which options are installed. These include: Stacker option Barcode reader options Temperature control Adjustable beam size...

  • Page 127: Measurement Technologies

    Tools menu Measurement technologies The final section of this tab tells what measurement technologies are installed and available: Fluorometry FP (Fluorescence polarization) Luminometry Photometry TR-Fluorometry (dissociation enhanced time-resolved fluorometry) LANCE (homogeneous time-resolved fluorometry)

  • Page 129: Exiting

    Exiting...

  • Page 131: File Menu

    Exiting File menu The File menu in the main window contains one item, Exit . This allows you to exit from the workstation software. You need to confirm the operation. If you try to exit while an assay run is taking place, you will get an error message telling that this is not possible.
  • Page 132 Exiting...

  • Page 133: Troubleshooting

    Troubleshooting...

  • Page 135: Out Of Memory

    Troubleshooting Out of memory If free disk space for the database drops below a specified limit, a warning message appears in the top right corner of the Manager window. A warning sound is also made once a minute. These warnings are continued until enough free disk space is available again. An assay run cannot be started from the user interface while the disk space is below the limit, however the Start button on the instrument still starts the run.

  • Page 136: Display Problems

    Performance degradation If you find your Wallac 1420 software is not running as fast as it used to, please run the Wallac 1420 Database Maintenance program. You should anyway do this on a regular basis.

  • Page 137: Processing Error

    Troubleshooting Check that no other major resource-consuming programs are running while using the Wallac 1420 software. Check that there is at least 30 MB available on your C: drive. Defragment your hard disk. You can find this function in your Windows Start menu under Programs\Acessories\System tools\Disk Defragmenter.
  • Page 138 Troubleshooting...

  • Page 139: Appendices

    Appendices...

  • Page 141: Appendix 1: Connecting Multicalc To Wallac 1420

    Appendix 1: Connecting MultiCalc to Wallac 1420 Appendix 1: Connecting MultiCalc to Wallac 1420 Introduction MultiCalc can read assay text files from Wallac 1420. Evaluation of assays can be fully automated. Measured wells are read sequentially. Plate printout and re-order work only for full plates.

  • Page 142: Installation Of Wallac 1420 Output Option In Multicalc

    Property O is for the connection type Offline. MultiCalc assay protocols All assays with Wallac 1420 have to be of the FLUORO type for all measurement technologies used in Wallac 1420. The response from labels 1 and 2 will appear as COUNTS and COUNTSB and from label 3 as COUNT.

  • Page 143: Appendix 2: Plate Types For Stackers

    Appendix 2: Plate types for stackers Appendix 2: Plate types for stackers All stackers are tested with following plates (until further notice given). Note: Lids or loose frames can not be used in the stacker Wallac 1244-550 DELFIA microtitration strip plates C554-160 Rigid low fluorescence plate C120-105...
  • Page 144 Appendix 2: Plate types for stackers Labsystems 95029550 Combiplate, white 9502817 Cliniplate, black Acceptable range of plate dimensions for plates in a 1420-216 Stacker: Length: 127.2 - 128.2 mm Width: 84.5 - 86.0 mm Height: 14.0 - 25.0 mm Ledge height: 1.5 - 6.5 mm Note: the length and width include the size of the ledge.

  • Page 145: Appendix 3: Dispenser Operation

    5 to 350 microlitres. Note: dispensing to 384-well plates is possible if just one pump is installed. The optional dispenser is a separate unit from Wallac 1420 itself making for convenient maintenance and flexibility in syringe selection. The information about installing the dispenser unit will be found in the Instrument manual as part of the installation instructions.

  • Page 146: Emptying The Waste Vial

    Appendix 3: Dispenser operation Emptying the waste vial Note: the waste vial must be empty when operation starts. To check that the waste vial is empty and if not, to empty it, proceed as follows. Open the top and front cover of the dispenser and then lift off the blue plate at the back of the dispenser.
  • Page 147 Empty out any liquid, following the disposal procedure recommended by the manufacturer of the reagent you have been using. Then replace the waste vial on its holder and slide it back into place. Replace the blue plate. This is important because it prevents light leakage to Wallac 1420.

  • Page 148: Preparing The Tubing

    Appendix 3: Dispenser operation Preparing the tubing Put the tubing into a container of water.

  • Page 149: Dispenser Maintenance

    Appendix 3: Dispenser operation Dispenser maintenance Note: in the enhanced security mode this tool is enabled only for users who belong to the Operator user group. Clicking the Dispenser maintenance icon or the item in the Tools menu invokes the Dispenser Maintenance Setup page to perform common dispenser maintenance operations.
  • Page 150 Appendix 3: Dispenser operation Note: before performing this operations make sure the dispenser has been prepared, as described earlier. Fill is used for filling the syringe and tubing before operation begins. Empty is used after operation ends to empty the contents of the syringe and tubing back into the bottle.
  • Page 151 Appendix 3: Dispenser operation Put a reagent bottle into the holder. Fit the tubing into the grip. Fit the end of the tubing into the reagent bottle. Click Fill to fill the tubing with reagent.

  • Page 152: Measurement

    Appendix 3: Dispenser operation Measurement Close the covers of the dispenser. The system should now be ready for operation. Proceed with the measurements.

  • Page 153: Maintenance

    Appendix 3: Dispenser operation Maintenance After measurement has been completed, perform the following maintenance operations: Click the Empty command. This will cause the reagent to be sucked back into the reagent bottle so that none is wasted. Open the covers. Remove the tubing from the reagent bottle and put it into a container of water and select the Flush command again.
  • Page 154 Appendix 3: Dispenser operation Avoid touching the needles with your fingers. To clean the needles use e.g. a stick with a tip of soft material dipped in ethanol, or use a spray bottle filled with ethanol. Be very gentle when touching the needles so that they are not bent out of shape. Rinse with water. The position of the needles is very critical for high quality performance.

  • Page 155: Appendix 4: Enhanced Security Special Functions

    Instrument manual for further information. User groups and permissions The enhanced security mode usage of Wallac 1420 is based on user groups and their permissions. There are five different user groups and each group contains permanent permissions. Groups and permissions are created during the installation and they cannot be added, removed or modified.

  • Page 156: User Management

    Note: User Management is only enabled for users who belong to the Administrators user group. With User Management the Wallac 1420 administrator can add, remove and edit users and manage the password control. Click the Add button to add users to Wallac 1420. The New User dialogue will appear.
  • Page 157 Appendix 4: Enhanced security special functions All the fields in the New User dialogue must be filled in. Status (enabled/disabled) sets the login status of the new user. Status “disabled” means that the user cannot log in to Wallac 1420 Manager. Status “enabled” (default) means that the user can normally log in to Wallac 1420 Manager.

  • Page 158: Password Control

    Appendix 4: Enhanced security special functions Password Control This tab allows the Wallac 1420 administrator to set the following password related settings. Maximum password age defines the maximum number of days that users can retain the same password before they must change it. By default the password expires after 42 days.
  • Page 159 Lockout defines the number of failed login attempts that are allowed before a user is locked out of Wallac 1420 Manager. For example, if “Number of failed logins allowed before lockout” is set to 5 failed login attempts, the user is allowed 5 failed attempts to log in. On the 5th failed attempt they are locked out until the Administrator allows them access again (Permanent) or for a specified period of time (Duration).

  • Page 160: Audit Trail

    Appendix 4: Enhanced security special functions Lockout duration defines the time spent after the maximum number of failed login attempts. The “Permanent, until administrator unlocks” – option button means that the user will be unable to log in again until the administrator has assigned a new password. The “Duration”...
  • Page 161 Appendix 4: Enhanced security special functions Clicking the Archive button launches the Archive dialogue (similar to the Save As dialogue) where the user can enter the path and filename of the archive text file. The Save command archives the audit trail and deletes the archived entries from the database. Only entries that are as old as or older than the selected date are archived.

  • Page 162: Archive

    Appendix 4: Enhanced security special functions Archive Note: Archive appears only when the enhanced security mode is installed. Archive is enabled only for users who belong to the Service user group. Archiving deletes results from the database and archives the deleted results as text files. The protocol parameters that were used with the assay run are also archived with the results.

  • Page 163: Security Settings

    Security is enabled only for users who belong to the Administrators user group. During the enhanced security set up, an internal Wallac 1420 application account is created. The Wallac 1420 Administrator can change the application account password by means of...
  • Page 164 Wallac 1420 program will fail to start. This is because the Wallac 1420 program has saved the application password internally and it does not match with the new system password. In this case the Wallac 1420 program forces the user to change the password at startup i.e. the Security Settings dialogue is shown at startup.
  • Page 165 Index...

  • Page 167: Index

    Beam size (Time-resolved fluorometry), 74 Dimensions of a plate, 120 Button light, 33 Dispense button (Measurement tab), 45 Button on Wallac 1420, 33 Dispenser information, 143 Buttons (Measurement tab), 45 Dispenser maintenance, 67, 147, 151 Buttons in the filters window, 90...
  • Page 168 Index Fill, 147, 148 Filter CW-Lamp, 76, 78, 83, 92 Editor, 153 Filter emission, 73, 76, 78, 80, 83, 87, 91 Eject slide button, 95 Filter excitation (LANCE), 86 Emission aperture, 77, 83 Filter excitation (TR fluorometry), 72 Filter properties, 99 Emission filter slide, 94 Filter slide settings changing, 98 Emission filters, 73, 76, 78, 80, 83, 87, 91...
  • Page 169 Index ID tab, 40, 43 Injector position during measurement, 47 Input/Output, 125 Measurement, 44 Installation of software, 13 Measurement (Protocol editor), 44 Install. of output option in MultiCalc, 140 Measurement height (Plate parameters), 48 Instrument Control, 18 Measurement mode Instrument name, 120 Plate, 44 Instrument options, 68, 124 Single well, 44, 47...
  • Page 170 Index Options installed, 124 Preparing the plate (LANCE norm.), 106 Orientation of strips, 120 Preview print, 55 Out of memory, 133 Print (Protocol editor), 55 Output, 11 Print (Result Viewer), 64 Outputs (Protocol editor), 50 Print preview (Protocol editor), 55 Outputs tab, 40, 50, 53 Print setup (Protocol editor), 55 File output, 51...
  • Page 171 User management, 154 Path, 46 Slide (emission filter) changing, 95 Slide aperture, 73, 77, 80, 83, 87 Slowdown in program, 134 Wallac 1420 MultiCalc assay protocols, 139 Software installation, 13 Warning, 135 Stabilized energy mode, 76 Waste vial, 144 Stacker, 28, 29, 31, 142...

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