Waters ACQUITY UPLC Overview And Maintenance Manual page 140

Attribute Specification
Composition ±0.5% absolute (full scale) from 5 to 90% from 0.5 to 2.0 mL/min, with
accuracy
2
i Valve
Test conditions:
• Mobile phase: degassed 90:10 acetonitrile/water; 90:10 acetonitrile/
• Back pressure: 13,790 kPa (138 bar, 2000 psi)
• Gradient conditions: Step gradient method
• Detector: UV, 273 nm wavelength
Composition <0.15% RSD or ±0.04 min SD, whichever is greater, based on six replicate
precision
injections, with i
Test conditions:
• Mobile phase: 60:40 water/methanol mixed via Auto•Blend Plus
• Flow rate: 0.5 mL/min
• Sample mix: alkylphenone mix (5.0 µL injection volume)
• Column: ACQUITY UPLC BEH C
• Column temperature: 35 °C ±0.3 °C
• Detector: UV, 254 nm wavelength
Compressibility Automatic and continuous
compensation
Priming Wet priming can run at flow rates up to 4 mL/min
Pump seal wash Equipped with a wash system, to flush the rear of the high pressure seal
and the plunger.
QSM: The default interval between seal wash pump activations is 5.0 min.
bioQSM: The default interval between seal wash pump activations is 0.1
min (6 s).
Flow ramping Range: 0.01 to 30.00 min to reach 2.0 mL/min
Default: 0.45 min, to reach 2.0 mL/min at 4.44 mL/min
Vent valve Used for priming the pump and automated leak testing. When the column
manager switches columns, the vent valve switches to the vent position, to
reduce system pressure.
Solvent lines Set of factory-installed inlet tubing assemblies. Each assembly includes a
10-µm reservoir filter.
Composition range 0.0 to 100.0% settable in 0.1% increments.
water with caffeine at 12 mg/L concentration
2
Valve
technology
August 8, 2016, 715005050 Rev. C
Page 140
, 1.7 µm, 2.1 × 50 mm
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