Overview Of Optimization Parameters - Thermo Scientific Corona Veo Operating Manual

Column Guidelines
6.7.2

Overview of Optimization Parameters

Charged Aerosol Detectors Corona Veo (RS) Operating Manual
•
If you use the detector for the analysis of complex biological
samples, proper sample preparation is especially important.
•
Minimize dead volumes before the detector. This is a commonly
overlooked but important cause of band broadening. Use capillaries
with a small internal diameter of the shortest length possible.
• Use a dedicated column for each specific analysis to avoid cross-
contamination of the column.
•
Columns that are unstable and/or have a tendency to release
particles from the column bed (i.e. "column bleed") will produce
noise in the detector. Avoid such columns.
When operated near the extremes of their pH value, temperature
and/or pressure operating range, columns may also be subject to
"column bleed".
•
Do not use a column that has been used with non-volatile mobile
phases.
•
Flush new columns to waste for several hours before you connect
them to the system.
•
If you perform a gradient, it is recommended to perform blank
injections with the desired method gradient profile prior to analysis.
This "pre-conditioning" of the column improves the performance of
both, the analytical column and the detector.
The following table serves as an overview of parameters that influence
the performance of the detector during data acquisition.
Parameter
Filter constant
Evaporation temperature
Power function value
Data collection rate
For further information about the parameters, refer to the Chromeleon
Help.
Affects
Sensitivity, baseline noise
Baseline noise, background current
Linear range
Peak resolution, disk space, possibly baseline noise
6 • Operation
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