Sectioning - Leica HistoCore AUTOCUT Instructions For Use Manual

Rotary microtome
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5
Operation
5.8

Sectioning

Warning
Personnel with insufficient qualification working with the instrument.
Serious injury of persons and or specimen damage can occur when specimen approaches knife/
blade due to inappropriate operator actions, for example, because object head may drop onto knife
holder when handwheel is unlocked.
• Always make sure that only laboratory personnel with dedicated and sufficient training and
qualification operates the instrument.
• Always make sure that all laboratory personnel designated to operate this instrument has read
these Instructions for Use carefully and is familiar with all technical features of the instrument
before start working with it.
Warning
Personal protective equipment is not used.
Injury of persons.
• When working with microtomes, personal safety precautions must always be taken. It is
mandatory to wear work safety shoes, safety gloves, a mask and safety goggles.
Warning
Working with the instrument and brittle specimen without appropriate protective clothes.
Serious injury of persons by splinters while cutting brittle specimen.
• Always wear appropriate protective clothes (including safety goggles) and work especially
carefully when cutting brittle specimen.
Warning
Insufficient frame conditions for sectioning.
Specimen damage or poor sectioning results,for example, sections alternating in thickness,
compressed, folded or stripped sections.
• Do not continue sectioning if you recognize insufficient sections results.
• Make sure that all prerequisites for appropriate sectioning are met. Refer to the troubleshooting
section of these Instructions for Use for further details.
• If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning
results consult persons who do have that knowledge, for example, application experts of Leica
Biosystems.
Warning
Turning the handwheel counterclockwise.
People injury/Damage to specimen
• Do not turn the handwheel counterclockwise, which may lead to handwheel locking mechanism
malfunction.
64
Version 1.1, Revision B

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