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8.
Daily operation
8.6
Adjusting the anti-roll guide
• Place the anti-roll guide on the knife holder during
trimming.
The anti-roll guide is correctly adjusted when
the section smoothly slides down between the
anti-roll plate and the knife (see illustration
below)!
Fig. 20
• Readjust the anti-roll guide if necessary.
• Decrease the section thickness continually to the
required value.
20
Correct adjustment of the anti-
roll guide
8.7
Sectioning and section transfer
• Decrease the section thickness continually to the
required value.
After changing from one section thickness to
another, the first two or three sections should
be rejected!
• Make sure the section smoothly slides down be-
tween the anti-roll plate and the knife.
• Remove the anti-roll guide from the knife for apply-
ing a section to a microscope slide.
Take care when removing the section - the
cutting edge is exposed!
The section can be transferred either to a precooled
microscope slide or to a microscope slide at room tem-
perature.
Section transfer to a slide at room temperature
• Carefully approach the slide to the cut section.
The frozen section 'flies' to the slide, rapidly thaws and
thus adheres to the slide surface making subsequent
orientation impossible.
Section transfer to a precooled slide
• Carefully apply the section to the slide by gentle
brushing.
• Orient and flatten the section on the slide surface
by means of the brush.
• To ensure the section tightly adheres to the slide
surface, warm the cold slide placing a finger against
the underside of the slide directly underneath the
section.
Instruction Manual V2.1 – 12/2001
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