Sectioning - Leica SM2010 R Instructions For Use Manual

Sliding microtome
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5
Operation
Note
Proceed the same way to clamp a blade rail.
5.6

Sectioning

Cutting into the specimen (trimming)
For trimming, the specimen feed can be disengaged either by turning the coarse driving wheel
(→ Fig. 37-4)
or by operating the manual feed lever
• Hold the knife sledge
specimen.
• Pull the knife guard
• To feed the specimen towards the knife, turn the coarse driving wheel
or
select the required section thickness with the section thickness adjusting knob
move the manual feed lever
selected value.
• Move the knife sledge forth and back until the specimen surface is trimmed as required.
5
4
Fig. 37
Remove the sections
Always use different areas of the cutting edge for trimming and sectioning.
• Select the required section thickness with the section thickness adjusting knob
• To produce the section, pull the knife sledge over the specimen at a constant speed.
• Carefully remove sections using a small brush and prepare them.
40
(→ Fig. 37-1)
at the grip
(→ Fig. 37-3)
of the blade/knife holder to the right.
(→ Fig.
37-6). Each lever movement causes a specimen feed by the
6
(→ Fig.
37-6).
(→ Fig. 37-2)
and place the sledge behind the
3
(→ Fig.
37-4);
(→ Fig. 37-5)
and
1
2
(→ Fig.
37-5).
Version 1.8, Revision I

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