Ise Calibration (Ph/Ise Module Only); Ise Calibration Editing - Thermo Scientific Orion Versa Star Pro User Manual


Chapter 4 pH, pH/ISE and pH/LogR Measurement Modules

56 Orion Versa Star Pro Meter User Manual

ISE Calibration (pH/ISE Module Only)

One to six standards can be used for ISE (ion selective electrode) calibration. If more than one
standard is used for calibration, start with the lowest concentration standard and work up to the
highest concentration standard last. Always use fresh standards. Select standards that bracket
the sample concentration and are one decade (10 times) apart in concentration. To accurately
prepare calibration standards from a stock solution, serial dilution is recommended using
calibrated pipettes. If ISA will be added to samples, the same ISA must be added to all
standards prior to calibration to ensure a consistent dilution factor.
Prepare the ISE according to the instructions in the electrode user manual. Connect the ISE
and any other electrodes to be used to the module inputs on the selected channel. In the
measurement mode, press the channel key until the channel with the pH/ISE module with ISE
connected is shown. If needed, press the mode key until ISE Measurement is shown as the
measure mode and use the
1. Press the f1 (Cal) key to start the calibration.
a. If more than one channel is displayed in the measurement mode, press the  or 
key to highlight the desired channel and press the f3 (Select) key.
2. Rinse the ISE and any other electrodes in use with distilled water or rinse solution required
for that electrode. Blot the electrode dry with a lint-free tissue or shake the electrode and
then place into the standard. Do not wipe or rub the sensing element of the electrode.
Follow all electrode rinsing and drying instructions or exceptions in the ISE user manual.
3. When the electrode and standard are ready, press the f3 (Start) key.
a. If using a stirrer probe, the stirrer probe will start stirring when the f3 (Start) key is
pressed and stop stirring when the reading stabilizes.
4. Wait for the concentration value to stabilize and perform one of the following actions:
a. Press the f2 (Accept) key to accept the displayed value.
or
b. Press the f3 (Edit) key and use the numeric keypad to enter the concentration value.
Press the f2 (Accept) key to confirm the entered value.
5. Press the f2 (Next) key to proceed to the next standard and repeat steps 2-4 or press the
f3 (Cal Done) key to save and end the calibration.
6. The meter will display the calibration summary including the slope and export the data to
the calibration log. Press the measure (esc) key to proceed to the measurement mode.

ISE Calibration Editing

Once the f3 (Cal Done) key is pressed and the calibration is saved, press the f2 (Cal Edit) key
to edit the calibration data and fix individual points without a full recalibration. In the calibration
edit mode, press the  or  key to highlight a calibration point and press the f2 (Delete) key
to delete the point or press the f3 (Re-Measure) key to re-measure the point. Follow the
onscreen text prompts and instructions to edit the selected calibration point.
ISE Mode Menu to set the Electrode Type.
Thermo Scientific