Esp Rna (Fast) - Beckman Coulter Optima L-XP Instructions For Use Manual

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The simulation is displayed on the graph.
• The green and blue curves represent the relative concentration of linear and supercoiled
DNA, respectively.
• The red curve represents the density of the CsCl gradient with respect to the right-hand
scale.
• The dotted line at the top of the graph represents the concentration at which CsCl will
precipitate.
Time required for the maximized separation is shown in the Elapsed Time (hr:min) window.
You can use the slide bar to display separations at shorter run times. When you find the
required separation with the shortest run time, you can transfer these parameters to the
Settings window and start the run, or save the simulation for later recall.
NOTE
To save the program for future recall, press
box. If the program is a modification of a previously saved program that you want to retain, you can
save the new version under a different name by selecting
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is automatically saved to a temporary file called LastESPRNA(Best).rnb.

ESP RNA (Fast)

The RNA (Fast) simulation, of a sample suspended in 4 M GuSCN without CsCl layered over a CsCl
cushion occupying one-fourth of the tube volume, is optimized for speed.
To set up an ESP RNA (Fast) run:
1
Select
ESP RNA Run (Fast)
2
Enter the particle length (see
3
Select the rotor (swinging bucket only) and tube in use.
PN LXP-IM-7AB
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.
Simulate Protocol
)
to transfer the run parameters to the Settings window.
Download
) ( )
, then to run the program. If not saved to a specific filename, the program
Enter Start
from the Simulate menu.
Figure
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Save , then enter a program name in the dialog
Save As
3.6).
Simulations
ESP RNA (Fast)
from the File menu.
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