Waters Xevo G2 QTof Operator's, Overview And Maintenance Manual page 76

5. While watching the camera image, carefully move the tip forward
toward the groove, until it touches, and a small piece of the glass
capillary shears off.
6. Return the sprayer to its previous position.
7. Ascertain that sample is flowing by observing a droplet on the tip.
If you cannot observe a droplet, increase the pressure briefly, up to
Tip:
a maximum of 1.5 bar, and then return the pressure to 0.3 bar.
8. If you observe a droplet, continue the procedure at
Requirement:
step 8.
If, after you repeat
droplet, discard the glass capillary and fit a new one (see
9. Set the capillary voltage slider to between 1.0 and 3.0 kV.
10. With an ion beam now visible on the peak display, optimize the sprayer
position and capillary voltage for maximum signal intensity.
The spray optimizes between 1.0 and 3.0 kV. To stop the sprayer,
Tip:
set the capillary voltage to 0.
4-14 Configuring the NanoLockSpray Source
Glass capillary
If you do not observe a droplet, repeat
step 4 through
Groove
step
step 4
step 8, you still cannot observe a
9.
through
page 4-11).