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•
Ultrasonic bath.
•
Source of oil-free, inert gas (for example, nitrogen) for drying (air-drying
optional).
•
Wash bottle containing HPLC-grade (or better) 1:1 methanol/water.
•
Large beaker.
To clean the extraction cone:
Warning:
biohazardous and/or toxic materials. Always wear
chemical-resistant, powder-free gloves while performing this
procedure.
Warning:
extreme care, use a fume hood and suitable protective equipment.
The extraction cone is fragile. Never place it on its tip; always
Caution:
place it on its flanged base.
1.
Immerse the extraction cone in the glass vessel containing 1:1
methanol/water.
If the extraction cone is obviously contaminated, use 45:45:10
Tip:
methanol/water/formic acid.
2.
Place the vessel in the ultrasonic bath for 30 minutes.
3.
If you used formic acid in the cleaning solution, do as follows:
a.
Rinse the extraction cone by immersing it in a glass vessel
containing water and then placing the vessel in the ultrasonic bath
for 20 minutes.
b.
Remove any residual water from the extraction cone by immersing
it in a glass vessel containing methanol and then placing the vessel
in the ultrasonic bath for 10 minutes.
Caution:
clean, chemical-resistant, powder-free gloves for the rest of this
procedure.
4.
Carefully remove the extraction cone from the vessel, and blow dry it
using inert, oil-free gas.
The extraction cone can be contaminated with
Formic acid is extremely corrosive and toxic. Work with
To avoid recontaminating the extraction cone, wear
Cleaning the extraction cone
5-43
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