Operation; Before Your Experiment - Merck Millipore CellASIC ONIX2 User Manual

8

Operation

8.1 Before Your Experiment

8.1.1 Prepare the ONIX Plate
8.1.2 Seal Plate to Manifold
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1. Prepare the ONIX Microfluidic Plate according to the instructions in the
Plate User Guide.
2. Depending on your experiment, optional steps at this point may include:
● Precoating chambers with ECM or priming channels with growth
medium
● Loading cells using passive surface tension methods
● Preculture in incubator using passive gravity flow to allow cells to
attach
1. Place the microfluidic plate on a flat surface.
2. Align manifold over the plate and make sure it is seated down against
the wells.
3. Press the Seal button and push down evenly with a slight force for
~5 seconds to ensure uniform contact during sealing. When a proper
seal is formed, the system will beep twice and the blue Seal light will
illuminate.
If a seal cannot be formed, check the gasket to make sure that it is
clean and properly aligned. Correct manifold operation can be confirmed
by using the included Self Check Plate. If you experience difficulty,
contact Technical Service.
Manifold Basic
Figure 24. Sealing plate to manifold
37 of 60
Manifold XT
CellASIC ONIX2 Microfluidic System
®