Troubleshooting - Merck Millipore CellASIC ONIX2 User Manual

10

Troubleshooting

General
Observation
1. System does not
power on
2. Error indicator
light blinks
3. Ready indicator
light blinks
4. All three lights
(Ready, Sealed,
and Error) flash
together
5. Difficulty sealing
manifold to plate
6. Plate fluid leakage
7. Fluid in the
manifold lines
20202092 04/19
Potential Cause
No AC power
Error condition
exists
Warning condition
exists
ONIX2 firmware has
crashed
Downward force not
applied evenly
Debris on gasket
Gasket misaligned
Plate surface
damaged
Loose Luer fittings/
filters
Luer fittings not in
correct order
Damaged plate
Culture chamber
outlet wells were
not emptied and
they overflowed
55 of 60
Recommended Action
Confirm that the power cable is securely
connected to the system and the power
outlet.
Verify that the outlet has power.
Make sure the ON/OFF switch on the rear
panel is in the ON position.
Check error/warning tray in ONIX2 Software
to see what the error is. Follow recommended
actions in Software Issues and Errors and
Warnings tables below.
Check error/warning tray in ONIX2
Software to see what the warning is. Follow
recommended actions in Software Issues and
Errors and Warnings table below.
Turn power off using ON/OFF switch on the
rear panel, then turn back on. If problem
persists, contact Technical Service.
Confirm recommended hand position as
described in Before Your Experiment.
Clean gasket as indicated in Maintenance and
Cleaning.
Make sure gasket is aligned as described in
Maintenance and Cleaning.
Inspect the plate for damage such as cracks
or chips to the surface. If damage is found,
contact Technical Service.
Tighten Luer fittings and filters as indicated in
Installation and Setup.
Connect Luer fittings consecutively along the
length of the Filter Multiconnector.
Check plate for cracks. If cracks are found,
contact Technical Service.
Clean manifold and tubing and replace filters
as described in Maintenance and Cleaning.
For subsequent experiments, choose a slower
flow rate and/or pause protocol midway to
empty waste outlets.
CellASIC ONIX2 Microfluidic System
®