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Merck Millipore Mobius Chrom 20 System User Manual

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User Guide
Mobius
Chrom 20 System with Flexware
®
®
Assemblies
The life science business of Merck KGaA, Darmstadt,
Germany operates as MilliporeSigma in the U.S. and Canada.

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Summary of Contents for Merck Millipore Mobius Chrom 20 System

  • Page 1 User Guide Mobius Chrom 20 System with Flexware ® ® Assemblies The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
  • Page 2 User Guide...

  • Page 3: Table Of Contents

    Mobius Chrom 20 System with Flexware Assemblies ® ® Contents System Overview Introduction ......................10 Mobius Chrom 20 System with Flexware Assemblies ........... 11 ® ® System Specifications....................12 Mobius Chrom 20 System with Flexware Assemblies ........... 12 ® ® Storage Requirements ....................
  • Page 4 User Guide Sample Operation Creation ..................90 Gradient Phase ......................92 Creating a Gradient Profile ..................93 Linear Gradient Test Operation................... 95 Peak Detection ......................96 Definition of Peak and Sub-peak Setpoints ..............96 Definition of Peak and Sub-peak Start and End Points ........... 96 UV Stability Time .....................
  • Page 5 Mobius Chrom 20 System with Flexware Assemblies ® ® Connecting the Flexware ® ................................138 Connecting the Flexware Assemblies ..............138 ® Connecting the Bubble Trap Assembly ............... 139 Connecting the Precolumn Filter Assembly ..............139 Connecting the Chromatography Precolumn Instrumentation Assembly ......140 Connecting the Post Column Instrumentation Assembly ..........
  • Page 6 User Guide Removing the Mobius Chrom 20 System with ® Flexware Assemblies ® System Preparation ....................193 Removing the Mobius Chrom 20 System with Flexware Assemblies ...... 194 ® ® Maintenance, System Conversion, and Troubleshooting Introduction ......................197 General Maintenance ....................198 Calibrating the Analog Instruments ................
  • Page 7 Mobius Chrom 20 System with Flexware Assemblies ® ® Limit Example ....................... 240 Suspending a Collection Group ................241 Modifying a Collection Group ................... 241 Deleting a Tag ....................... 241 Modifying a Tag ..................... 241 Deleting a Collection Group ..................241 Saving Changes to Historical Assign Configuration ............

  • Page 8: System Overview

    User Guide System Overview...
  • Page 9 Mobius Chrom 20 System with Flexware Assemblies ® ®...

  • Page 10: Introduction

    User Guide Introduction Mobius Chrom 20 System with Flexware Assemblies are fully automated industrial systems ® ® that deliver optimal operational flexibility. Unlike traditional single-use technology, the systems are quick and easy to set-up, requiring only a minimum number of components. The system utilizes Flexware single-use flow paths that minimize the risk of cross ®...

  • Page 11: Mobius ® Chrom 20 System With Flexware ® Assemblies

    Mobius Chrom 20 System with Flexware Assemblies ® ® Mobius Chrom 20 System with Flexware ® ® Assemblies The Mobius Chrom 20 System with Flexware Assemblies is a fully automated chromatography ® ® system designed to achieve optimum separation and purification of monoclonal antibodies, vaccines, plasma and therapeutic proteins while offering a high degree of operational flexibility.

  • Page 12: System Specifications

    User Guide System Specifications Mobius Chrom 20 System with Flexware ® ® Assemblies Mechanical Specifications Mobius Chrom 20 System with Flexware Assemblies for Chromatography ® ® Specification Mobius Chrom 20 System ® System Pump & base carts connected 1727 x 1715 x 800 + 20 mm Dimensions H x W x D in mm Base with Flexware...
  • Page 13 Mobius Chrom 20 System with Flexware Assemblies ® ® Specification Mobius Chrom 20 System ® Flow Range (L/min) 4 to 20 Pump Control Fixed Position (speed in %) or Flowcontrol Operating Specifications Mobius Chrom 20 System with Flexware Assemblies for Chromatography ®...
  • Page 14 User Guide Specification Mobius Chrom 20 System ® 6 bar minimum, 10 bar maximum, oil free Pneumatic Air Base Supply Maximum consumption 4 L/min Instrument Specifications Tags refer to the labels on the system hardware. Mobius Chrom 20 System with Flexware Assemblies for Chromatography ®...

  • Page 15: Storage Requirements

    Mobius Chrom 20 System with Flexware Assemblies ® ® Automation Specifications Specification Mobius Chrom 20 System ® Allen Bradley Compact Logic Control software Windows 10 Operating System ® Control Platform system Operator iFix Software ® interface panel type Operator Interface 19 in.

  • Page 16: System Components

    User Guide System Components BASE WITH INSTRUMENTATION AND BUBBLE TRAP SUPPORT Tag (Labeled on the System) Description Number none Fixed Wheel none Swivel Wheel with Lock none Column Instrumentation Mounting Pins Flexware Clamshell Assembly Power ® XC009 Connector none Bubble Trap Support ES001 Emergency Stop USB001...
  • Page 17 Mobius Chrom 20 System with Flexware Assemblies ® ® Mobius Chromatography System Base (front view) ®...
  • Page 18 User Guide (Labeled Description Number on the System) PI010 System Air Pressure Gauge USB003 Print Port XC001 Electrical Power Connection XC002A Pump Base Electrical Connection XBUSA Murr Communication to Base ETH010 Network Connection (connect to user domain, printers, security, etc.) ETH002A Tank Cart Ethernet Connection ETH001...
  • Page 19 Mobius Chrom 20 System with Flexware Assemblies ® ® Mobius Chromatography System Base (rear right view) ®...
  • Page 20 User Guide Component Number (Labeled on the System) LSH001 Bubble Trap High Level Sensor LSL002 Bubble Trap Low Level Sensor XPUMP2 Pump 2 1200 Electrical Connection XPUMP1 Pump 1 1200 Electrical Connection None Hook Inter Base Locking Device None Inter Base Centring None Wheel Directional Lock Mobius...
  • Page 21 Mobius Chrom 20 System with Flexware Assemblies ® ® Flexware Clamshell Assembly ® Component Number (Labeled on the System) ZS001, ZS002, ZS003, ZS004, ZS005, ZS006, Internal Lock with Sensor (7 places) ZS007 None Centring Pin for Base Cart None Pin for Flexware Clamshell Assembly Lift ®...
  • Page 22 User Guide Component Number (Labeled on the System) XV030 Normally Closed Valve XV031 Normally Open Valve XV033 Normally Closed Valve XV034 Normally Closed Valve PCV032 Normally Open Control Valve XV028 XV024 XV021 XV025 XV022 XV029 XV026 XV020 XV023 XV030 XV033 XV031 XV014 XV016...
  • Page 23 Mobius Chrom 20 System with Flexware Assemblies ® ® PUMP CART WITH MANIFOLD, PUMPS AND FLOWMETERS Component Number (Labeled on the System) ES002 Emergency Stop FT002 Flowmeter SU P002 Pump P002 Support FT001 Flowmeter SU P001 Pump P001 Support IB05 Manifold Box None M Box...
  • Page 24 User Guide Component Number (Labeled on the System) XP003 Emergency Release Button FT002 Flowmeter Connector FT001 Flowmeter Connector XC002B Electrical Connector XBUSB Murr Communication to Base IB04 Pump Base Electrical Box XP002B Compressed Air Inlet (from Base) 801SP2 Pneumatic ON/OFF Switch None Pbase Centring None...

  • Page 25: Piping And Instrumentation Diagram (P&Id) Legend

    Mobius Chrom 20 System with Flexware Assemblies ® ® Piping and Instrumentation Diagram (P&ID) Legend Component (Labeled on the System) Pump Pump Speed Variator Automatic Valve Pressure Control Valve Pressure Reducing Valve Temperature Sensor + Function Pressure Sensor + Function Level Sensor Flow Sensor Analyzer...
  • Page 26 User Guide Symbol Description Symbol Description Mixer Plug Pinch Clamp Millipak Filter ® (valve identification tags for P&ID reference only) Opticap Filter Control Valve ® Pellicon Holder ® R=Retentate Port, F=Feed Normally Open On/Off Valve Port, P=Permeate (High Port), PD=Permeate Drain HB Connector 2D Container TC Connector...
  • Page 27 Mobius Chrom 20 System with Flexware Assemblies ® ®...

  • Page 28: Assembling And Setting Up The Hardware

    User Guide Assembling and Setting Up the Hardware...
  • Page 29 Mobius Chrom 20 System with Flexware Assemblies ® ®...

  • Page 30: Introduction

    User Guide Introduction The Mobius Chrom 20 System with Flexware Assemblies is a modular system that includes ® ® the pump cart with the manifold and the base. Assemble and connect the bases in the order presented in this chapter. Note Before installation of the system ensure that the area is free of any objects and there is enough space to install the system.

  • Page 31: Assembling The Pump Cart

    Mobius Chrom 20 System with Flexware Assemblies ® ® Assembling the Pump Cart Connecting the Pumps 1. Unpack P001 pump cable and connect it to connector P001 on the base cart 2. Unpack P002 pump cable and connect it to connector P002 on the base cart...

  • Page 32: Sliding The Pumps

    User Guide Sliding the Pumps 1. Loose P001/P002 pump front and rear screws 2. Adjust P001/P002 pump position by sliding to left or right 3. Tighten P001/P002 pump front and rear screws...

  • Page 33: Connecting The Flowmeters

    Mobius Chrom 20 System with Flexware Assemblies ® ® Connecting the Flowmeters Installing the Single-use Flowmeters 1. Install the flowmeter supports onto the pump support with the screws and nuts on the flowmeter support, using a no. 6 socket wrench. Flowmeter Flowmeter support Shelf mounting screws...
  • Page 34 User Guide Assembled Pump Cart with Single-use Flowmeters...

  • Page 35: Assembling The Base

    Mobius Chrom 20 System with Flexware Assemblies ® ® Assembling the Base Installing the Instrument Holders Single-use instruments require different holders. The appropriate holder must be hooked on the top and bottom of the base side panel. Holder Pre-column Single-use Instrumentation with Holder Protection Holder Post-column Single Use Instrumentation with holder...

  • Page 36: Installing The Bubble Trap (Bbt) Support

    User Guide Installing the Bubble Trap (BBT) Support 1. Hook the Bubble Trap Support (BBT001 support) onto the pins located on the left side of the Base. Bubble Trap Support Pins Installing the BBT Support on the Base Installing the Keyboard Holder Hook the keyboard holder onto the pin located on both sides of the Flexware Clamshell ®...

  • Page 37: Installing The Pre-Column Filter Support

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing the Pre-column Filter Support Filter Support Location 1. Place the pre-column filter support on the floor. Pre-column Filter Support...

  • Page 38: Connecting The Cart

    User Guide Connecting the Cart To connect the two cart, position the base and lock the wheels. Push the pump cart towards the base until the inter-base locks slide together and engage. Turn the thumbscrews on the lock clockwise to tighten. Lock the pump base wheels. Inter-Cart Locking Device Connecting the Pump Cart with the Base...

  • Page 39: Connecting To Power, Pneumatic And Ethernet Sources

    Mobius Chrom 20 System with Flexware Assemblies ® ® Connecting to Power, Pneumatic and Ethernet Sources 1. Connect XP001 on the base to an appropriate compressed air source with ≥ 6 bar pressure. 2. Connect XP002A on the base to XP002B on the pump base. 3.

  • Page 40: Power Up

    User Guide Power Up Check that emergency stop button is unlocked. Turn the power supply switch to “ON” position on main electrical box MB01. The power indicator should light up. Supervision screen, PC and PLC should then be under power. PC should start loading programs. Application launches automatically.

  • Page 41: Shut Down

    Mobius Chrom 20 System with Flexware Assemblies ® ® Shut Down From main screen touch “user” icon (see below) and select shut down windows. After the screen is off, turn the power supply switch to the “OFF” position. The blue light and the white lights should turn off.
  • Page 42 User Guide...

  • Page 43: Installing The Flexware ® Clamshell Assembly

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing the Flexware Clamshell ® Assembly The base is delivered with one Flexware Clamshell Assembly installed. The Flexware Clamshell ® ® Assembly can be removed and replaced with a different unit if required. Install and connect the Flexware Clamshell Assembly in the order presented in this guide.

  • Page 44: Removing The Flexware Clamshell Assembly In A Base

    User Guide Removing the Flexware Clamshell ® Assembly in a Base Refer to the Common Control Platform Overview section of this manual for instructions on ® logging in to the system and navigating through the screens. 1. After logging on to the system, select the Recipe Pool icon. 2.

  • Page 45: Installing The Flexware ® Clamshell Assembly Into An Empty Base

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing the Flexware Clamshell ® Assembly into an Empty Base The base and the pump cart must be fully assembled, locked together and connected to an appropriate power and compressed air sources before proceeding. Remove the plug on the Flexware Clamshell Assembly power cord before installing the Flexware Clamshell Assembly...
  • Page 46 User Guide...

  • Page 47: Using The Common Control Platform

    Mobius Chrom 20 System with Flexware Assemblies ® ® Using the Common Control Platform ® Software...
  • Page 48 User Guide...

  • Page 49: Starting The System

    Mobius Chrom 20 System with Flexware Assemblies ® ® Starting the System This system is for industrial use only and cannot be used in a residential environment. Once the system is installed, power up the system. Powering up the system starts up the Common Control Platform (CCP ) software.
  • Page 50 User Guide If an attempt is made to gain access to the user interface with unauthorized login information, an unauthorized access message will appear. After logging in, the login prompt closes and the process display opens. Note If login at startup has been disabled from the system settings screen, then this login prompt does not appear and Process display opens directly instead.

  • Page 51: User Interface

    Mobius Chrom 20 System with Flexware Assemblies ® ® User Interface The user interface includes a tool bar, piping and instrumentation diagram (P&ID) and various user selectable status displays. The user must have the appropriate security privileges to open certain displays. There are five main sections of the User Interface: •...

  • Page 52: Navigation Bar

    User Guide Navigation Bar Navigation bars organizes the controls as shown in the following table. The buttons on the tool bars carry out specific actions. Click on the desired tool bar button to perform the action. Button Description No action Opens the Recipe Editor to edit operations and procedures.

  • Page 53: Changing The Language

    Mobius Chrom 20 System with Flexware Assemblies ® ® Changing the Language Languages other than English may be specified when a system is ordered. The Change Language icon in the USER MENU opens the Language Selection screen. There are eight different language options. When the language is changed, the texts of interface at User Interface, Recipe Editor and Report Client will all be changed to the selected language.

  • Page 54: Process Display (P&Id)

    User Guide Process Display (P&ID) The P&ID screen dynamically displays all process conditions, analog and digital values and pump status. Thanks to the display setup screen, it is possible to choose whether to show sensor tags or labels as well as displaying sensor locations.

  • Page 55: Piping

    Mobius Chrom 20 System with Flexware Assemblies ® ® Button Description Set the system to its default state (confirmation required). The default state includes control of valves and flow path: • Pump off • All manifold inlets closed • Bubble trap online •...

  • Page 56: Digital 2-Way Valves

    User Guide Digital 2-way Valves Clicking on a digital 2-way valve symbol opens its control popup. Auto Mode Symbol Manual Mode Symbol Description Closed Valve Open Valve Control Valves Symbol Description Fully Closed Control Valve (100% Clo) Partially Open Control Valve Fully Open Control Valve (0%Clo) Non Critical Fault Critical Fault...

  • Page 57: Pumps

    Mobius Chrom 20 System with Flexware Assemblies ® ® Inlet Flow Path Buttons To place an inlet on-line, click on the desired inlet button (labelled 1A-1E or 2A-2E). When Gradient Control is Off and an inlet is clicked to open, the related flow path is selected and all other inlets are closed.

  • Page 58: Pump Control

    User Guide Pump Control When pump speed is controlled by Flowrate or Linear Velocity, then a circle is added to the pump symbol and shows the current control mode: Symbol Control Mode Pump Speed Controlled by Flowrate Pump Speed Controlled by Linear Velocity Pump Speed Controlled by Mixing.

  • Page 59: Filter

    Mobius Chrom 20 System with Flexware Assemblies ® ® Auto Vent Symbol Description Auto Vent is enabled. The vent valve is automatically controlled in order to maintain liquid in the bubble trap at its operating level. The valve opens when liquid is below the low-level sensor. The valve closes when liquid is above the high-level sensor.

  • Page 60: Column

    User Guide Column Clicking on a Column symbol opens its control detailed panel. Column – Flowpath Symbol Description No valid flowpath is selected Column Forward flowpath is selected Column Reverse flowpath is selected...

  • Page 61: Process Controls Bar

    Mobius Chrom 20 System with Flexware Assemblies ® ® Process Controls Bar The content of the Process Controls Bar varies based on the current system status (alarm presence, recipe running, …). Symbol Description START Starts the current recipe. ABORT Aborts the current recipe. HOLD Sets the system to hold state.

  • Page 62: Recipe Status And Totalizers

    User Guide Symbol Description Opens parameters save and restore popup. Opens the recipe pool popup. The recipe pool includes open/close door recipes and lock/ unlock Flexware Clamshell Assembly recipes. ® Recipe Status and Totalizers The Recipe Status and Totalizers display shows process information: •...
  • Page 63 Mobius Chrom 20 System with Flexware Assemblies ® ® When a recipe is running, the left part of the display shows the following information for each phase tab: • the number of the phase • the number of the step •...

  • Page 64: Recipe Pool Screen

    User Guide Recipe Pool Screen The Recipe Pool screen has 6 Recipe buttons and a Launch/Configure Mode tab. • In Configure mode, each of the 6 Recipe buttons can be configured with an existing recipe. In Launch mode, if no other operation is running when a given Recipe button is clicked, the associated recipe is downloaded and run.

  • Page 65: Using The Recipe Editor

    Mobius Chrom 20 System with Flexware Assemblies ® ® Using the Recipe Editor Introduction The Recipe Editor is where operations (sequences of actions) and procedures (sequences of operations) are created and managed. The building blocks of operations are individual actions. A series of actions are listed sequentially to form an operation.

  • Page 66: Launching The Recipe Editor

    User Guide Launching the Recipe Editor Launch the Recipe Editor by clicking on the Recipe Editor icon in the User Interface. The main window of the editor opens as shown below. Tool Bar functionality of the icons in the Recipe Editor Toolbar is described below. Icon Description Create a new procedure or edit an existing one...
  • Page 67 Mobius Chrom 20 System with Flexware Assemblies ® ® Icon Description Switches to Work space. Edit Phase 0 Edit Phase 1 Edit Phase 2 Edit Phase 3 Edit Phase 4 Edit Phase 5 Edit Linear Gradient (for Chrom 20 only) Open On-line Manual Steps The numbered rows in the Operation Area are the steps of the operation.
  • Page 68 User Guide Creating a Step 1. Click anywhere on the line in the tabular environment in which the action is to be added/ inserted. The line should now be highlighted in yellow. 2. Click on the appropriate action group in the Select Action Group list on the top left side of the Recipe Editor.
  • Page 69 Mobius Chrom 20 System with Flexware Assemblies ® ® The buttons on the top right-hand side of the application environment perform the functions described in the following table. Icon Description Transfers the selected action onto the highlighted step. Any action previously displayed as the highlighted step is transferred to the next step.
  • Page 70 User Guide Saving Operation To Save the operation, click the Save icon in the Recipe Toolbar or click Operation and then Save As in the Recipe Editor menu bar. Type a name for the operation in the subsequent dialog box. The operation will be stored with an “.opn”...
  • Page 71 Mobius Chrom 20 System with Flexware Assemblies ® ® Running the Operation To run the operation downloaded to the PLC, exit the recipe editor by clicking on the Exit icon in the Recipe Editor toolbar and return to the Process Display. Note Always check the Process Display for proper system status before running an operation.
  • Page 72 User Guide Edit a Procedure To Edit an existing Procedure, click Open on the Edit Procedure Form. A window opens with a list of existing Procedures. Click on the Procedure that must be edited and click OK. The Edit Procedure Form is shown with the Operations in the Procedure section populated with the operations that are in the procedure.
  • Page 73 Mobius Chrom 20 System with Flexware Assemblies ® ® Recipe Editor- Actions Summary The following tables gives the list of the available actions for each action group: Flowpath-Inlets Inlet 1B Flow Path Inlet 1C Flow Path Flowpaths Inlet 1D Flow Path Inlet 1E Flow Path Inlet 2A Flow Path Inlet 2B Flow Path...
  • Page 74 User Guide Valve Control XV005 Close XV006 Open XV006 Close XV007 Open XV007 Close XV008 Open XV008 Close XV009 Open XV009 Close XV010 Open XV010 Close XV011 Open XV011 Close XV012 Open XV012 Close XV013 Open XV013 Close XV014 Open XV014 Close XV015 Open Open and close valves...
  • Page 75 Mobius Chrom 20 System with Flexware Assemblies ® ® Valve Control XV025 Open XV025 Close XV026 Open XV026 Close XV028 Open XV028 Close XV029 Open XV029 Close Open and close valves XV030 Open XV030 Close XV031 Open XV031 Close XV033 Open XV033 Close XV034 Open XV034 Close...
  • Page 76 User Guide Flow Control-Pump1 Pump P001 Stop Pump P001 Run Pump P001 Control by Speed Pump P001 Control by Volumetric Flow Rate Pump P001 Control by Linear Velocity Pump P001 Speed Set Point Pump P001 Volumetric Flow Set Point Pump P001 Linear Velocity Set Point P001 Control: Pump P001 Ramp Rate Start/Stop commands...
  • Page 77 Mobius Chrom 20 System with Flexware Assemblies ® ® Flow Control-Mixing Mixing Enable Mixing Disable Mixing Start Pumps Mixing Stop Pumps Mixing Control Pumps by Speed Mixing Control Pumps by Volumetric Flow Mixing Control Pumps by Linear Velocity Mixing Control Pumps Speed Set Point Mixing Control Pumps Volumetric Flow Set Point Mixing Control Pumps Linear Velocity Set...
  • Page 78 User Guide Flow Control-Mixing Mixing TotalFlow Deviation HIHI Alarm Mixing Conductivity Deviation Alarm Enable Mixing Conductivity Deviation Alarm Disable Mixing Control: Mixing Conductivity Deviation HI Alarm Start/Stop commands Mixing Conductivity Deviation HIHI Alarm Control loop modes and related setpoints Control loop parameters Linear Gradient Start Deviation alarms Linear Gradient Abort...
  • Page 79 Mobius Chrom 20 System with Flexware Assemblies ® ® PostColumn-Peak Detection Peak Inflection Detection Enable Peak detection control: Enable/disable peak detection Peak Inflection Detection Disable Sets end of peak detection mode either based on an absolute value or based on a percentage of the height Peak Inflection Percentage Sets start and end setpoints...
  • Page 80 User Guide Analog Alarms Enable AI102 Pre column conductivity Alarm Disable AI102 Pre column conductivity Alarm AI102 Pre column conductivity LOLO AI102 Pre column conductivity LO AI102 Pre column conductivity HI AI102 Pre column conductivity HIHI Enable AI103 Pre column pH Alarm Disable AI103 Pre column pH Alarm AI103 Pre column pH LOLO AI103 Pre column pH LO...
  • Page 81 Mobius Chrom 20 System with Flexware Assemblies ® ® Analog Alarms Disable AI203 Post column UV1 Alarm AI203 Post column UV1 LOLO AI203 Post column UV1 LO AI203 Post column UV1 HI AI203 Post column UV1 HIHI Enable AI204 Post column 1 UV2 Alarm Disable AI204 Post column 1 UV2 Alarm AI204 Post column UV2 LOLO AI204 Post column UV2 LO...
  • Page 82 User Guide Analog Alarms AI01 Exchange AI#1 Alarm HI AI01 Exchange AI#1 Alarm HIHI Enable AI02 Exchange AI#2 Alarm Disable AI02 Exchange AI#2 Alarm AI02 Exchange AI#2 Alarm LOLO AI02 Exchange AI#2 Alarm LO AI02 Exchange AI#2 Alarm HI Enable AI03 Exchange AI#3 Alarm Enable/Disable analog alarms Disable AI03 Exchange AI#3 Alarm Sets analog alarms setpoints...
  • Page 83 Mobius Chrom 20 System with Flexware Assemblies ® ® Discrete Alarms DF48VP001 Pump 1 VDC defect Alarm Disable DF48VP002 Pump 2 VDC defect Alarm Enable DF48VP002 Pump 2 VDC defect Alarm Disable LV01_Err Pump1 Linear Vel. SP Alarm Enable LV01_Err Pump1 Linear Vel. SP Alarm Disable LV02_Err Pump2 Linear Vel.
  • Page 84 User Guide Discrete Alarms PI002 Signal Failure Alarm Disable FI002 Signal Failure Alarm Enable FI002 Signal Failure Alarm Disable PI003 Signal Failure Alarm Enable PI003 Signal Failure Alarm Disable AI102 Signal Failure Alarm Enable AI102 Signal Failure Alarm Disable AI103 Signal Failure Alarm Enable AI103 Signal Failure Alarm Disable AI201A Signal Failure Alarm Enable AI201A Signal Failure Alarm Disable...
  • Page 85 Mobius Chrom 20 System with Flexware Assemblies ® ® Miscellaneous Sets the volume of fluid held in the system Holdup Volume flowpath Flags the start of HETP data to be used by HETP Start the HETP report. Flags the end of HETP data to be used by the HETP End HETP report.
  • Page 86 User Guide Operations and Phase Control Phase 1 Start Phase 1 Stop Phase 1 Pause Phase 1 Resume Phase 2 Start Phase 2 Stop Phase 2 Pause Phase 2 Resume Start Starts phase X Phase 3 Start Stop Stops phase X Phase 3 Stop Pause Pauses phase X...
  • Page 87 Mobius Chrom 20 System with Flexware Assemblies ® ® Recipe Editor- Criteria Summary The criteria in the Recipe Editor environment are intended to be self-explanatory. Criterion Description This criterion (empty field) makes the transition true, i.e., the action associated None with the step where this criterion stands will always be executed.
  • Page 88 User Guide Exchange AI#3 Exchange AI#4 Exchange DI#1 Exchange DI#2 Analog and digital values can be compared to Exchange DI#3 a user defined value through mathematical operators (=, >=, <=) in order to produce the Exchange DI#4 desired criterion. Exchange DI#5 Exchange DI#6 Exchange DI#7 Exchange DI#8...
  • Page 89 Mobius Chrom 20 System with Flexware Assemblies ® ® FLC002 Pump2 within deadband FLC002 Pump2 Control Stable Valve PCV032 Ramp Complete Valve PCV032 within deadband Valve PCV032 Control Stable Goto Default on End Enabled Checks if Goto Default on complete is enabled Goto Default on End Disabled Checks if Goto Default on complete is disabled Criteria Flag 1 On...

  • Page 90: Sample Operation Creation

    User Guide Phase 4 Total Time Phase 4 Total Volume Phase 4 Total CVs Phase 5 Total Time Phase 5 Total Volume Phase 5 Total CVs Checks the answer buttons associated with a Phase 0 Prompt OK message-based dialog box. Phase prompt criteria may be used only in the next step after a step with a prompt that is Phase 0 Prompt Yes...
  • Page 91 Mobius Chrom 20 System with Flexware Assemblies ® ® Set the system to Global Auto and Default (Step 1 & 2) at the start of an operation, to ensure that the status of components is known. CAUTION Valves that have been manually forced (not set on Auto) will not be affected by the System Default.

  • Page 92: Gradient Phase

    User Guide Gradient Phase One of the capabilities of the system is the ability to form gradients based on conductivity. It allows a gradient to be defined in terms of the conductivity (in μS) of the fluid entering the column. The software employs a hybrid feed-forward-feedback algorithm to manipulate the gradient valve to achieve the desired conductivity.

  • Page 93: Creating A Gradient Profile

    Mobius Chrom 20 System with Flexware Assemblies ® ® The user can create the desired gradient profile in two ways: • graphically by clicking and dragging the symbols (l and n) in the graphical area • by entering the appropriate values of the gradient basis (Y) and gradient variable (X) in the table below the graph.
  • Page 94 User Guide The programmed gradient is shown here: Executing a Gradient from an Operation For an operation to execute a programmed gradient, the following actions should be listed in the order given below in the appropriate phase of the operation. The Actions are found in the Gradient Action Group in the Operation screen.

  • Page 95: Linear Gradient Test Operation

    Mobius Chrom 20 System with Flexware Assemblies ® ® Linear Gradient Test Operation Steps in Phase 0 Step Criteria 1 Action Value Global Auto Mode Go to Default Inlet 1A Flow Path Step Time ≥ 2 seconds Bubble Trap Online Flow Path Step Time ≥...

  • Page 96: Peak Detection

    User Guide Peak Detection The peak detection capability of the system is based on the absolute values of the UV absorbance. The user can set the UV absorbance for the start of the peak and the end of the peak and can trigger actions based on their attainment. In addition, the software allows for the detection of sub-peaks within the main peak (e.g., shoulders on the front or tail of the main peak).

  • Page 97: Uv Auto Zero

    Mobius Chrom 20 System with Flexware Assemblies ® ® UV Auto Zero The UV Auto Zero function establishes an artificial zero (baseline), based on the current value of the UV signal. The resulting offset remains in effect until the original zero is re-established, when the function is turned off.

  • Page 98: Remote Desktop Connection

    User Guide Remote Desktop Connection Remote Desktop Connection enables connection to a remote computer. For administrator details, refer to the Computer Administration document in the digital set of documents supplied with the system.

  • Page 99: Batch Reporting

    Mobius Chrom 20 System with Flexware Assemblies ® ® Batch Reporting Introduction The CCP system provides the collection, storage, processing and reporting of batch ® production data, in accordance with international standards on batch control and current good manufacturing practices. All recorded information pertaining to a batch is referred to as the batch history.
  • Page 100 User Guide Icon Description Switches to Work space. Open On-line Manual Allow user to customize the toolbar Generating a Report Batch reports are created using the Report Generator. Click the Report Generator or, on the Report Client menu bar, click on Reports then Report Generator. The Report Generator form appears.
  • Page 101 Mobius Chrom 20 System with Flexware Assemblies ® ® Note The string “12” is present in all Run ID descriptors present in this column. Select a Run To select a run for report generation, click the appropriate row (in any column) that contains the run information needed.

  • Page 102: Report Configuration

    User Guide Report Configuration Report Configuration Menu The report configuration menu allows the user to save, recall and activate report configurations. Note Only users with appropriate security privileges can save configurations. Saving a Configuration Click the Save button and enter a unique name. The current configuration will be saved for future recall.

  • Page 103: Configuring Analog Data

    Mobius Chrom 20 System with Flexware Assemblies ® ® Configuring Analog Data When either the Full Report or the Historical Trend boxes are checked within the Report Selections box, the Configure button is enabled. The Configure button allows the user to define which sensor data archived by the unit is actually included within a particular report.

  • Page 104: Automatic Report Generation

    User Guide Configuring HETP Report When either the Full Report or the Calculated Values boxes are checked within the Report Selections box, the Configure HETP button is enabled. The Configure HETP button opens the HETP Report Configuration Screen. The user may then define the parameters for HETP calculations, including the Start and End Points, Analysis Mode and HETP Variable.

  • Page 105: Report Preview

    Mobius Chrom 20 System with Flexware Assemblies ® ® Report Preview The final report can be previewed by clicking the Preview Report button on the top left corner of the Report Generator form. A sample of the final reports is shown below. Click the Table of Contents icon on the tool bar to see the contents of a report.
  • Page 106 User Guide Recipe Step Report Event Report...
  • Page 107 Mobius Chrom 20 System with Flexware Assemblies ® ® Alarm Report Security Report...
  • Page 108 User Guide Tabular Data Report...

  • Page 109: Printing Reports

    Mobius Chrom 20 System with Flexware Assemblies ® ® Analog Data Report Printing Reports To print the report to the default printer, click the Print Report button on the top left corner of the Report Generator form. Saving Reports A report can be saved in different formats: •...

  • Page 110: Opening An Existing Report

    User Guide Opening an Existing Report Existing reports can be opened within the CCP Report Client by clicking the Open Report Icon ® or by clicking Reports menu and the Open Report menu item. The Open Report window opens. To open a report, select the file from the list on the right side of the form and click OK. The File Name can be typed in the text box provided on the bottom of the form.

  • Page 111: Customizing The Report Client Menu Bar

    Mobius Chrom 20 System with Flexware Assemblies ® ® To check the integrity of the files residing in different locations within the available drives and folders, click the File Path button located on the top left corner of the Verify Tamperproof Signature form.

  • Page 112: Historical Trend Display

    User Guide Historical Trend Display Introduction The Historical Trend Display helps the user monitor the performance of the process. The basis of the Historical Trend Display is the Chart Group File, which contains a list of tag names for which data is being collected.
  • Page 113 Mobius Chrom 20 System with Flexware Assemblies ® ® The maximized displayed can either show one single trend chart or three stacked charts. Note A specific chart group can be selected for each trend. Symbol Description Select either 1 single chart or three stacked charts Gives access to the following choices: •...

  • Page 114: Chart Group File

    User Guide Chart Group File The basis of the Historical Trend is the Chart Group. The Chart Group File is a *.csv file containing a list of database tag names for which data is being collected. Chart Group files are located in the folder: XMO4 by Chrom20.

  • Page 115: Data Sources: Pen Selection And Editing

    Mobius Chrom 20 System with Flexware Assemblies ® ® Section Functionality Chart Group File Lists the name of the chart group file that is being configured Displays the pen list of the current chart group file. Allows Data Sources the user to select additional data (pens) from the Historical Database Time Sets the time limits (X-axis range) for the data display...
  • Page 116 User Guide Using the Expression Builder Window The Expression Builder window has five tabs. Only the Historical tab is needed. Clicking the Historical Tab button opens the historical database with the list of historical assigned and collected pens. To add a pen using the Expression Building Window: 1.

  • Page 117: Time: Setting Time Range For The Pens

    Mobius Chrom 20 System with Flexware Assemblies ® ® Mathematical Function Keypad Editing the Pen List Use the Remove button to remove an existing pen from the pen list. Use the Move Pen Up or Move Pen Down button to change the position of a pen in the pen list. Time: Setting Time Range for the Pens The Time section of the Chart Configuration window allows the user to set the time range for pens.

  • Page 118: Data: Setting Limits

    User Guide Fixed Start Time uses the fixed start time or sets a start time. Clicking the Time field opens the keyboard. The Duration box chooses the duration time for the data display. Clicking the Duration data field opens the keyboard. The maximum duration time is 99:99:99:99. Data: Setting Limits The Data section in the chart group configuration window is used to set the value (Y-axis) range for the selected pen.

  • Page 119: Line Style Drop Down Menu

    Mobius Chrom 20 System with Flexware Assemblies ® ® Line Style Drop Down Menu When the Line Style drop down menu is highlighted, the selected style will be used to display the data profile line. Historical Mode Drop Down Menu The Historical Mode drop down menu is used to choose the way the data is displayed.

  • Page 120: Action Buttons: Completing The Configuration

    User Guide Action Buttons: Completing the Configuration When finished with the configuration of the Chart Group File, use the action buttons to save the file or to directly apply the pen selected and configured. Action Buttons in Chart Group Configuration window Button Action Clicking the New button opens a confirmation Chart Group...

  • Page 121: Deleting A Chart Group File

    Mobius Chrom 20 System with Flexware Assemblies ® ® In the Chart Group Configuration window: 1. The name of the file that will be edited appears in the Chart Group File section, e.g., XMO4 by Chrom20. 2. All the pens included in the file are listed in the Data Sources section. Use the Pen Edit buttons to add or remove a pen or adjust its position up or down.

  • Page 122: Editing Pens

    User Guide Editing Pens 1. Click the Edit Pens button in the Chart Group File window. This will launch the Chart Group Configuration window. If the required pen is not on the list, the user will have to open the list of all the pens.

  • Page 123: Analog Scaling

    Mobius Chrom 20 System with Flexware Assemblies ® ® Analog Scaling The EGU scaling and EGU units can be modified using Configure Analog Points tool from the CCP Recipe Editor application (see Recipe Editor chapter for further details). Start the Configure Analog Points tool by clicking on the Tools\Configure Analog Points menu. This feature is password protected and is synchronized with the Windows Security system.
  • Page 124 User Guide To change a value, click on the cell. A popup is displayed to fill a value. For a number: For a string: Pressing the Save button will initiate a sequence of updating of the Configuration file (*.cfg), PLC configuration and SCADA configuration. A record will be logged into the SQL Database. Pressing the Cancel button allows to close the tool without making any changes.

  • Page 125: Installing The Mobius

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing the Mobius Chrom 20 ® with Flexware ® Assemblies...
  • Page 126 User Guide...

  • Page 127: Introduction

    Mobius Chrom 20 System with Flexware Assemblies ® ® Introduction Before installing any Mobius Chrom 20 with Flexware Bag Assembly, verify that the Base and ® ® the Pump Cart are located on a level surface, locked together, and wheel locks are engaged. Power to the system should be ON.

  • Page 128: Installing The Mobius ® Chrom 20 System With Flexware

    User Guide Installing the Mobius Chrom 20 ® System with Flexware Assemblies ® This section details the installation of the Mobius Chrom 20 System with Flexware Assemblies ® ® that must be installed onto the Base before any connections can be made. The next section details connecting all the Flexware Assemblies used on the system.

  • Page 129: Bottom Manifold Assembly Onto The Manifold

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing Mobius Chrom 20 System with ® Flexware Assemblies for Chromatography Pump ® 1 Bottom Manifold Assembly onto the Manifold XM4P1MTC The Pump Manifold Assemblies are available with TC fittings. All fittings are shipped with a cap installed.

  • Page 130: Manifold Assembly Onto The Manifold

    User Guide Installing Mobius Chrom 20 with Flexware ® ® Assemblies for Chromatography Pump 2 Top Manifold Assembly onto the Manifold XM4P2MTC The Pump Manifold Assemblies are available with TC fittings. All fittings are shipped with a cap installed. Remove the covering just before connecting the flow path to the system. Connect TO P002 Pump Manifold Assembly XM4P2MTC...

  • Page 131: Installing The Pump Heads Onto The Pumps

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing the Pump Heads onto the Pumps DISPUMP2 1. Remove the stainless steel flange (if present) from the pumps. 2. Note the flow direction on the pump heads and install the pump heads on the pumps so that the inlet is on the left.

  • Page 132: Installing The Su Flowmeter Tube

    User Guide Installing the SU Flowmeter Tube FROM P001 Flowmeter Assembly XM4P1FLWSU FROM P002 Flowmeter Assembly XM4P2FLWSU The flowmeter supports (including the transmitter) must be installed onto the pump support. The SU flowmeter assemblies will be connected to the Flexware Clamshell Assembly later.

  • Page 133: Installing The Bubble Trap Assembly

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing the Bubble Trap Assembly XM4BUBBL The Bubble Trap Support must be installed onto the Flexware Base. The Bubble Trap Assembly ® will be connected to the Flexware Clamshell Assembly later. ®...

  • Page 134: Installing The Precolumn Filter Assembly

    User Guide Installing the Precolumn Filter Assembly XM4FILT001 The Precolumn Filter Assembly must be installed onto the Flexware Base. It will be connected ® to the Flexware Clamshell Assembly later. ® Filter Assembly XM4FILT001 1. Unpack the Precolumn Filter Assembly. This drawing and the data contained thereon is confidential THIS DRAWING IS USER GUIDE VIEWS...

  • Page 135: Installing The Flexware ® Clamshell Assembly Into The Clamshell

    Mobius Chrom 20 System with Flexware Assemblies ® ® Installing the Flexware Clamshell Assembly into the ® Clamshell XM4SMART Note The Flexware Clamshell Assembly can be used for 50 cycles (50 ® valve openings and 50 valve closings) per valve. The integrity of the installation may be compromised if used for more than 50 cycles.
  • Page 136 User Guide This drawing and the data contained thereon is confidential THIS DRAWING IS and the property of MilliporeSigma, Billerica Massachusetts, USER GUIDE VIEWS USA. Third Parties may use this document only to review THE PROPERTY OF and approve the drawing for use by MilliporeSigma in its MILLIPORESIGMA manufacturing.

  • Page 137: Installing The Single-Use Precolumn And Post Column Instrumentation Assemblies

    Mobius Chrom 20 System with Flexware Assemblies ® ® 8. Close the Flexware Base door using the Lock Door recipe on the Recipe Pool screen. ® 9. Hold the door closed on the top right corner of the Flexware Clamshell Assembly until the ®...

  • Page 138: Connecting The Flexware

    User Guide Connecting the Flexware ® There are a number of Flexware Assemblies that must be connected to the Flexware Clamshell ® ® Assembly. The previous section details the installation of these assemblies. This section details connecting all the Flexware Assemblies used on the system.

  • Page 139: Connecting The Bubble Trap Assembly

    Mobius Chrom 20 System with Flexware Assemblies ® ® Connecting the Bubble Trap Assembly XM4BUBBL The Bubble Trap Assembly must be connected to the Flexware Clamshell Assembly. ® The Bubble Trap Assembly 1. Remove the plugs/caps from the connectors labeled on the Flexware Clamshell Assembly.

  • Page 140: Connecting The Chromatography Precolumn Instrumentation Assembly

    User Guide Precolumn Filter Assembly By-pass (If no filter is used) 1. Remove the plugs/caps from the connector labeled on the Flexware Clamshell Assembly. ® 2. Connect to H. 3. Remove the plugs/caps from the connector labeled on the Flexware Clamshell Assembly.

  • Page 141: Connecting The Post Column Instrumentation Assembly

    Mobius Chrom 20 System with Flexware Assemblies ® ® Connecting the Post Column Instrumentation Assembly XM4PSTSU-1, XM4PSTSU-2 This assembly will be connected to the Flexware Clamshell Assembly. ® Single-use Post Column Instrumentation Assembly 1. Remove the plugs/caps from the tubing labeled on the Flexware Clamshell Assembly.
  • Page 142 User Guide Connecting the Column Assembly 2. Remove the dust bag from the connectors labeled on the assembly and on the Flexware ® to L. Clamshell Assembly. Connect 3. Remove the dust bag from the connectors labeled on the assembly and on the Flexware ®...

  • Page 143: Application Information

    Mobius Chrom 20 System with Flexware Assemblies ® ® Application Information...
  • Page 144 User Guide...

  • Page 145: Introduction

    Mobius Chrom 20 System with Flexware Assemblies ® ® Introduction This section of the user guide includes Specifications, P&ID drawings for some typical process steps and Flow Rate vs Pressure curves.

  • Page 146: Flow Paths

    User Guide Flow Paths The following pages contain P & ID for the sample flow paths listed here: Flowpath 1 Inlet Pump P001, BBT online, filter on line, column forward, waste Flowpath 2 Inlet Pump P001, BBT online, filter on line, column bypass, waste Flowpath 3 Inlet Pump P001, BBT online, filter on line, column reverse, waste Flowpath 4...
  • Page 147 Mobius Chrom 20 System with Flexware Assemblies ® ® Flowpath 1: Inlet Pump P001, BBT online, filter on line, column forward, waste...
  • Page 148 User Guide Flowpath 2: Inlet Pump P001, BBT online, filter on line, column bypass, waste...
  • Page 149 Mobius Chrom 20 System with Flexware Assemblies ® ® Flowpath 3: Inlet Pump P001, BBT online, filter on line, column reverse, waste...
  • Page 150 User Guide Flowpath 4: Inlet Pump P002, BBT online, filter on line, column forward, waste...
  • Page 151 Mobius Chrom 20 System with Flexware Assemblies ® ® Flowpath 5: Inlet Pump P002, BBT bypass, filter bypass, column forward, waste...
  • Page 152 User Guide Flowpath 6: Inlet Pump P001, BBT online, filter online, column forward, fraction...

  • Page 153: System Pressure Drop

    Mobius Chrom 20 System with Flexware Assemblies ® ® System Pressure Drop Test were performed with RO water at 200 C and the following parameters: One Pump Running Parameter Value Bubble Trap XM4BUBBL. Column None installed. Replaced by 1 M of tubing. Flow Rate Read from system flowmeter.
  • Page 154 User Guide Two Pumps Running Parameter Value Bubble Trap XM4BUBBL. Column None installed. Replaced by 1 M of tubing. Flow Rate Read from system flowmeter. Inlet 1A and 2A Pump P001 and P002 Fraction Waste Pressure Drop - Both pump running - mixing 50% Flowrate (LPM) BBT online filter online BBT online filter bypass...

  • Page 155: Using The System

    Mobius Chrom 20 System with Flexware Assemblies ® ® Using the System...
  • Page 156 User Guide...

  • Page 157: Introduction

    Mobius Chrom 20 System with Flexware Assemblies ® ® Introduction This section provides instructions on the use and functionality of the Mobius Chrom 20 System ® with Flexware Assemblies. Please review this section, in its entirety, before operating the ® system.

  • Page 158: Preparing The System For Operation

    User Guide Preparing the System for Operation The system settings display, available from the User menu with administrator rights, gives access to general system settings: Login Request at Start This setting allows to choose if user login is requested when starting the system. When placed to OFF, after startup sequence completion, the Process display is opened.

  • Page 159: Confirmation And Signature Window Configuration

    Mobius Chrom 20 System with Flexware Assemblies ® ® Confirmation and Signature Window Configuration This setting controls the confirmation box that appears before applying any user request (starting an equipment, changing a setpoint, …). Setting Description User request is directly applied. No confirmation box No confirmation window appear.

  • Page 160: Save Recipe Signature

    User Guide Save Recipe Signature This setting controls if user login is required for saving recipe files. When ON, the user login prompt appears when clicking on the save button of the Recipe Editor: Database Capacity The control system is periodically monitoring the SQL database capacity and triggers a non- critical alarm when the current size overpass this setting.

  • Page 161: Q Factor

    Mobius Chrom 20 System with Flexware Assemblies ® ® Q Factor To achieve the best accuracy on the flow measurement, each flowmeter electronic is factory calibrated. It is important to enter the Qmax factor into the HMI each time a new SU flowmeter electronic is changed.

  • Page 162: Windows Access

    User Guide Windows Access Open a Windows Files Explorer popup. Maintenance Switch to the maintenance mode (Access to this mode is restricted to those with maintenance privileges). Log Rate Log Rate button allows to access to the Historical Trend Assign utility. See section IV.2.2 “Starting Historical assign program”...

  • Page 163: Flowpath Configuration

    Mobius Chrom 20 System with Flexware Assemblies ® ® Flowpath Configuration Use the Flowpath Config. Button to open the flowpath configuration screen and allow the user to configure: • The default flowpath • The 5 user flowpaths 1. Select the Flowpath to be configured from the Select a Flowpath dropdown menu. 2.

  • Page 164: Process Control

    User Guide Process Control Process Control is achieved through the Process Display and its detailed panels. The process display is split in 4 areas: • Flow Control • Pre-column • Column • Post-column Each area has a detailed panel which can be accessed by clicking on the related button available at the bottom of the related process section of the process display.

  • Page 165: Flow Control

    Mobius Chrom 20 System with Flexware Assemblies ® ® Flow Control Pump 1 & Pump 2 Section Each pump detailed panel allows the following: • Select the pump control mode and its related setpoint • Select auto or manual mode •...
  • Page 166 User Guide The Loop Tuning popup shows the control loop values on a chart and allow to tune the control parameters in order to reach the desired dynamic properties. Ramp rate is used outside the window covered by the PID control loop. Fixed speed (Non PID controlled mode): the pump speed shall ramp until the speed setpoint is reached.
  • Page 167 Mobius Chrom 20 System with Flexware Assemblies ® ® To achieve the best accuracy on the flow measurement, it’s important to enter the K factor into the HMI for each SU flowmeter tube. The K factor is written on the label attached to each SU flowmeter tube.
  • Page 168 User Guide One of the capabilities of the system is the ability to form gradients based on conductivity. It allows a gradient to be defined in terms of the conductivity (in μS) of the fluid entering the column. The software employs a hybrid feed-forward-feedback algorithm to manipulate the gradient valve to achieve the desired conductivity.
  • Page 169 Mobius Chrom 20 System with Flexware Assemblies ® ® The Mixing Loop Tuning popup shows the control loop values. Tune the control parameters to reach the desired dynamic properties. Use the Primary and Secondary conductivity snap buttons to store the current conductivity reading as primary or secondary conductivity value.
  • Page 170 User Guide Procedure for Conductivity Calibration Conductivity calibration can be performed manually from the touch screen or automatically using an operation. Manual actions are performed using the Gradient Conductivity Calibration and Gradient Percentage Calibration windows. 1. Setup the desired flow path. 2.

  • Page 171: Pre-Column

    Mobius Chrom 20 System with Flexware Assemblies ® ® Pump Outlets Section The pumps outlets flowpath can be selected through this display: Pre-column Bubble Trap Section The Bubble trap panel allows to set the related flowpaths and to enable/disable the auto venting function.

  • Page 172: Column

    User Guide PCV Section The Pressure Control Valve has a detailed panel which allows the following: • Set its position setpoint • Set the ramp rate • Open/Close it fully through a single button The ramp rate set the rate, in %/sec, at which the CV will increase or decrease to get the desired PV.

  • Page 173: Post Column

    Mobius Chrom 20 System with Flexware Assemblies ® ® The table below indicates which valves are open in each selected flow path. Flow Path XV020 XV021 XV022 XV023 XV024 XV025 XV026 Forward Open Open Open Open Reverse Open Open Open Open Bypass Open...
  • Page 174 User Guide The UV Tuning popup shows the UV Value on a chart and allow to tune the UV detection algorithm: • The UV Probe to be used for detection can be chosen • End peak calculation mode can be set to absolute or percentage of the peak height •...

  • Page 175: Flexware ® Clamshell Assembly Settings

    Mobius Chrom 20 System with Flexware Assemblies ® ® Flexware Clamshell Assembly Settings ® The manual Door and Flexware Clamshell Assembly Control window is open by clicking on the ® Clamshell Settings tab in the Navigation Toolbar. Caution Improper setting of the valves may cause injury to the operator or damage to the Flexware Clamshell Assembly.
  • Page 176 User Guide Safety Before starting operations in manual control, the following actions will make sure that the system is in a safe state: 1. Disable the alarms ZS008, YA08 and YA09 and ZSDF. 2. Open all valves in the Flexware Clamshell Assembly.

  • Page 177: Display Setup

    Mobius Chrom 20 System with Flexware Assemblies ® ® Display Setup Display Setup can be accessed from the Process Controls Bar. It allows the User to: • Choose its display preferences for the Process display (Instruments labels or tags; View or hide sensors locations) •...
  • Page 178 User Guide Color Meaning Steady grey Blinking grey Waiting for operator answer Steady orange Non-critical acknowledged alarm is present Blinking orange Non-critical unacknowledged alarm is present Steady red Critical acknowledged alarm is present Blinking red Critical unacknowledged alarm is present Screensaver Settings The Screensaver settings popup allows the following •...

  • Page 179: Alarm Control

    Mobius Chrom 20 System with Flexware Assemblies ® ® Alarm Control The system includes alarm logic which provides safety to personnel and equipment, and ensures proper operating parameters are maintained. General Alarm Behavior Alarms are classified as non-critical (medium priority) or critical (high priority). Non-critical alarms provide warnings.
  • Page 180 User Guide Alarm Status – Pumps & Pre-column Alarm Status – Column & Post-column Alarm Status – Digital & Network...
  • Page 181 Mobius Chrom 20 System with Flexware Assemblies ® ® Alarm Signal – Exchange Click on one of these categories to open the Alarm settings panel. The History tab groups all current alarms on one list. Current alarms are also displayed on the Alarm banner. Alarms are acknowledged by selecting the Alarm Acknowledge icon on the bottom of the screen.
  • Page 182 User Guide The following categories contain analog alarms: • Pumps • Pre-column • Column • Post-column • Exchange To set up an alarm value, click on the appropriate cell (LOLO, LO, HI or HIHI). A data entry box will appear. There is a range for each process alarm. Minimum Range Value ≤LOLO ≤LO ≤...
  • Page 183 Mobius Chrom 20 System with Flexware Assemblies ® ® The Alarm priority can be set by clicking on the Critical or Non-Critical button. This always require a confirmation from the user, regardless the System Setting that has been set. Safety alarm priorities can be set. Safety alarms are always Critical and cannot be disabled. Safety alarms are the following: •...
  • Page 184 User Guide Resuming from Hold Once all Critical alarm conditions have been cleared, the user can resume the system from hold state using the Process Controls bar button: If a Critical alarm is still present, resuming from hold is not possible and the following message will appear: If no Critical alarm is present, resuming from hold triggers this popup: The first option «...

  • Page 185: Security Overview

    Mobius Chrom 20 System with Flexware Assemblies ® ® Security Overview The security system consists of group accounts, user accounts and security areas. A typical system uses four group accounts: Operator, Supervisor, System Administrator and QA Users. Each user of the system belongs to a group account corresponding to the user’s permitted access level.

  • Page 186: Priming The System

    User Guide Priming the System...
  • Page 187 Mobius Chrom 20 System with Flexware Assemblies ® ®...

  • Page 188: Introduction

    User Guide Introduction Once the Flexware Assemblies are installed and before operating the column, the tubing must ® be free of air and filled with liquid. The user should review Using the System to be familiar with the Common Control Platform (CCP ) Software.
  • Page 189 Mobius Chrom 20 System with Flexware Assemblies ® ® 1. Set Pump P002 control to manual control. Set the speed setpoint to 20%. The speed should be set at the lowest level that will allow sufficient flow to purge the lines of air. 2.
  • Page 190 User Guide At this point, the flowpaths from all of the inlets to the drain should be purged of air. 1. Confirm that a collection vessel is properly installed at the waste outlet and at the filter vent. 2. With the pump still running and one inlet open, the bubble trap will be set in auto mode. 3.

  • Page 191: Removing The Mobius

    Mobius Chrom 20 System with Flexware Assemblies ® ® Removing the Mobius Chrom ® 20 System with Flexware Assemblies ®...
  • Page 192 User Guide...

  • Page 193: System Preparation

    Mobius Chrom 20 System with Flexware Assemblies ® ® System Preparation Prior to removing the Mobius Chrom 20 System with Flexware Assemblies from the system, ® ® verify the following: • The product has been recovered into the collecting container •...

  • Page 194: Assemblies

    User Guide Removing the Mobius Chrom 20 ® System with Flexware Assemblies ® 1. Disconnect the column inlet and outlet. 2. Disconnect the pre column instrumentation cell. 3. Disconnect the post column instrumentation cell. 4. Remove the two instrumentation cells from their supports. 5.

  • Page 195: Maintenance, System Conversion, And Troubleshooting

    Mobius Chrom 20 System with Flexware Assemblies ® ® Maintenance, System Conversion, and Troubleshooting...
  • Page 196 User Guide...

  • Page 197: Introduction

    Mobius Chrom 20 System with Flexware Assemblies ® ® Introduction The Unload Flexware Clamshell Assembly for Maintenance recipe should be used when ® performing any maintenance procedures. The Load Flexware Clamshell Assembly After ® Maintenance recipe should be used to reinstall the back Flexware Clamshell Assembly after ®...

  • Page 198: General Maintenance

    User Guide General Maintenance Before doing any work, read and understand Operator and Equipment Safety. The following schedule is only a guideline. Depending on use, components may need more frequent maintenance. Maintenance Schedule Schedule Maintenance Procedures System Cleaning General Inspection Every Three Months Mechanical Preventive Maintenance Electrical Preventive Maintenance...

  • Page 199: Calibrating The Analog Instruments

    Mobius Chrom 20 System with Flexware Assemblies ® ® Calibrating the Analog Instruments Note Calibration events are not automatically recorded as electronic records by CCP software. For information on compliance with 21CFR211.68, refer to installation SOPs. Adjust the calibration of analog instruments using the Scaling popup on the user interface display.

  • Page 200: Cleaning The Hardware

    User Guide Cleaning the Hardware Note Read Operator and Equipment Safety in this user guide before performing any maintenance or troubleshooting. All parts of the system should be wiped down with a cloth dampened with one of the following agents: •...

  • Page 201: Moving The System

    Mobius Chrom 20 System with Flexware Assemblies ® ® Moving the System Before moving the system, the air reservoir must be emptied. The reservoir acts as an air buffer when the emergency stop is engaged. The air tank is located behind the Flexware Clamshell ®...

  • Page 202: Changing The Valve Pads

    User Guide Changing the Valve Pads The valve pads in the Flexware Clamshell Assembly should be changed after 2500 cycles or ® after six months of use. 1. Run the Unlock Door recipe to open the door and access the valve pads. 2.
  • Page 203 Mobius Chrom 20 System with Flexware Assemblies ® ® Valve Pads Valve Pad Description Size 5/8 inch...

  • Page 204: Replacing The Fitting Clips

    User Guide Replacing the Fitting Clips Flexware Clamshell Assembly Fitting Clip Size ® Mobius Chrom 20 with Flexware Assembly 5/8 inch ® ® 1. Run the Unload Flexware Clamshell Assembly for Maintenance recipe. ® 2. Remove the clip from the Flexware Clamshell Assembly by removing the two screws that ®...

  • Page 205: Replacing The Flexware

    Mobius Chrom 20 System with Flexware Assemblies ® ® Replacing the Flexware Clamshell ® Assembly Door O-rings or Balls The lock on the Flexware Clamshell Assembly has O-rings and stainless-steel balls that may need ® periodic replacement. O-ring Balls 1. Run the Unlock Door recipe to open the door. 2.

  • Page 206: Setting The Level Sensors

    User Guide Setting the Level Sensors The level sensors situating on the bubble trap support need to be set. A tip (a pen for example), a bubble trap filled half way with water and the level sensors are required. 1. The system should be powered ON and all emergency stops should be acknowledged. 2.

  • Page 207: Calibration Verification For The Pressure Sensors In The Flexware

    Mobius Chrom 20 System with Flexware Assemblies ® ® Calibration Verification for the Pressure Sensors in the Flexware ® Clamshell Assembly Material Required • An air source (a hand pump or compressed air) • A calibrated pressure sensor • A Flexware Clamshell Assembly for Chromatography ®...

  • Page 208: Pressure Sensors Pi001 And Pi002

    User Guide Pressure Sensors PI001 and PI002 1. Connect the air source to the calibrated pressure sensor and to the Flexware Clamshell ® Assembly as shown. 2. Close the valves as shown. 3. Apply pressure to reach the setpoints 0.00, 2.00 and 4.00 bar. 4.
  • Page 209 Mobius Chrom 20 System with Flexware Assemblies ® ®...

  • Page 210: Adding Or Removing Column Instrumentation

    User Guide Adding or Removing Column Instrumentation Adding Precolumn Instrumentation to a System with Post Column Instrumentation Only 1. Install the precolumn instrumentation hardware. See the Assembling and Setting Up the Hardware chapter in this manual for installation details. 2. Connect the conductivity, pH and UV cables. 3.
  • Page 211 Mobius Chrom 20 System with Flexware Assemblies ® ® System Settings Menu with LAMP VOLTAGE Selected 9. Set the LAMP VOLTAGE to ON and save it. Entering LAMP VOLTAGE 10. Select SYSTEM SETTINGS > SENSOR TEMPERATURE. The SENSOR TEMPERATURE menu appears.
  • Page 212 User Guide Sensor Conductivity Menu 14. Set parameters as follows: Parameter Settings Sensor COND1 COND2 Sensor Active Calibration User Temperature Compensation Reference Temperature 25.0000 Temperature Coefficient 2.0000 15. Click SAVE. 16. Select SYSTEM SETTINGS > SENSOR pH. The SENSOR pH menu appears. Sensor pH Menu 17.

  • Page 213: Date Time Format

    Mobius Chrom 20 System with Flexware Assemblies ® ® Date Time Format Choosing any format other than the ones listed below could cause errors when using CCP software: ® • MM-DD-YYYY • DD-MM-YYYY • YYYY-MM-DD Once the date format chosen, changing it afterwards may cause damage to the historical data. Before any change, it is recommended to create data backup and to purge the database.

  • Page 214: Troubleshooting

    User Guide Troubleshooting Component Symptom Corrective Action Ensure that system is plugged in to an electrical source and switched on. Ensure that the main disconnect is not turned off. No system Ensure that energy supply specifications are met. components are operating Ensure that the Emergency Stop is not engaged and the reset and there is no emergency/ HP blue light is illuminated.
  • Page 215 Mobius Chrom 20 System with Flexware Assemblies ® ® Component Symptom Corrective Action Verify that there are no other critical alarms on the Touchscreen. Verify that the PC and PLC connections and cables are not faulty. YA06 alarm is Verify that the power LCD on the switch is green. If it is trigged orange, the problem is with the switch.
  • Page 216 User Guide Component Symptom Corrective Action Verify that ZS008 is not defective before opening door. Verify Door could not Base Door that ZS008 is operational, using a metallic tool before starting be opened the recipe. Check valves are all opened and all fittings are mounted correctly. Verify that a ball is not missing in one of the locks.
  • Page 217 Mobius Chrom 20 System with Flexware Assemblies ® ® Component Symptom Corrective Action Verify that the four screws on the flange on the pump cover are correctly tighten. Verify that all connections located before pump are tight. Pump flow rate Verify that there are no kinks in the tubing.
  • Page 218 User Guide Component Symptom Corrective Action All alarms from the instruments connected to the C8000 transmitter are regrouped is this alarm. XC102-3DF is Check the C8000 transmitter for which alarm in triggered. See probe manufacturers documentation for more information. C8000 Defect All alarms from the instruments connected to the C8000 transmitter are regrouped is this alarm.
  • Page 219 Mobius Chrom 20 System with Flexware Assemblies ® ® System Administrator Information for the Common Control Platform Software ®...
  • Page 220 User Guide...

  • Page 221: Security

    Mobius Chrom 20 System with Flexware Assemblies ® ® Security Introduction The Common Control Platform (CCP Software) system security is configured using Windows ® ® ® 10 Security and the iFIX Security Configuration programs. This means that there are two ®...

  • Page 222: Security Configuration Report

    User Guide Security Configuration Report The export feature of the iFIX Security Configuration program can be used to obtain a ® Security Configuration Report. The export feature creates an ASCII file that lists configuration information for groups, users, etc. To obtain a Security Configuration Report: 1.

  • Page 223: Security Recommendations

    Mobius Chrom 20 System with Flexware Assemblies ® ® Security Recommendations Before using the system: • It is recommended to perform qualification of Inputs/Outputs and their settings. • Persons who will use or manage the system have to be trained and qualified. Procedure and training recommendations: •...
  • Page 224 User Guide • Note that the backup function does not save the recipes. For this backup make a copy from the directory XMO4 by Chrom20. Use frequently recipe backup operation. For more information, refer to the Computer Administration document in the digital set of documents supplied with the system.

  • Page 225: Shutting Down The Control System

    Mobius Chrom 20 System with Flexware Assemblies ® ® Shutting Down the Control System Caution Close all programs and shutdown Windows before switching off power to the ® computer. Failure to do so may damage any Historical Data files that are open when the computer loses power.

  • Page 226: Archive And Restore Utilities

    User Guide Archive and Restore Utilities Overview The CCP software provides an Archive Utility to back up and restore the numerical and event ® data acquired during operation of the unit. Data backed up by the archive utility include all the data that are stored in the Historian database (trending information) and SQL database (events).

  • Page 227: Archive Utility Screen

    Mobius Chrom 20 System with Flexware Assemblies ® ® Archive Utility Screen There are three main parts of the Archive Utility screen: • Database File Locations • Backup/Restore Database • Purge Database. At the bottom of the screen is a report box for progress and messages. Database File Locations Display the Current Active Data Drive and the Backup File Location.
  • Page 228 User Guide Backing Up a Database Each backup action generates one backup directory with a unique name. That directory name records the exact time of the backup. Depending on the size of the archive files and the backup location, the backup process may take considerable time.
  • Page 229 Mobius Chrom 20 System with Flexware Assemblies ® ® Before starting the restoration operation, users will be prompted to confirm the overwrite of their current active databases. Click OK to proceed with the restore, click Cancel to abort the process and keep the currently active databases. At the end of the successful restore operation the message “Successfully.

  • Page 230: Managing Ifix Software Data Collection

    User Guide Managing iFIX Software Data Collection...
  • Page 231 Mobius Chrom 20 System with Flexware Assemblies ® ®...

  • Page 232: Historical Assign Program

    User Guide Historical Assign Program The data collection software in CCP software has been configured for customer use. The ® Historical Assign program is to assign database blocks to be collected for use with the historical trending software. It should be used only by properly trained and authorized users. This Chapter describes how to: •...
  • Page 233 Mobius Chrom 20 System with Flexware Assemblies ® ® The data collections groups are listed below. Group Data Deadband PI001 0.01 PI002 0.01 PI003 0.01 FI001 0.01 FI002 0.01 AI201B PCV032 TI201 AI01 AI02 AI03 AI04 SIC001_AO SIC002_AO PCV032_AO AO01 AO02 AO03 AO04...
  • Page 234 User Guide Group Data Deadband BED_HEIGHT COLUMN_VOLUME FI003 LV003 Chart Mark AI102 AI103 0.01 AI201A AI202 0.01 AI203 0.001 AI204 0.001 The limit field provides a deadband limit to eliminate recording data within the specified range of the last recorded value. This prevents the database from filling up with data from signal noise and changes smaller than the limit.

  • Page 235: Starting Historical Assign Program

    Mobius Chrom 20 System with Flexware Assemblies ® ® Starting Historical Assign Program Historical assign is started when iFIX is started at power up. ® To edit Historical Assign, administrator access is required and a keyboard must be plugged into system.

  • Page 236: Creating A New Collection Group Assign Program

    User Guide Creating a New Collection Group Assign Program To create a historical collection group from the Historical Assign window, select Add from the Group menu. The Group Configuration dialog box opens and lists the tag names and the collection parameters for the group being created. Group Configuration Parameters Parameter Description...

  • Page 237: Select A Node

    Mobius Chrom 20 System with Flexware Assemblies ® ® 1. Select a Node In the Node field in the Group Configuration dialog box, enter the name of the SCADA software node that contains the block tag names for the collection group. To display a list of the SCADA software nodes being communicated with, click the “…”...

  • Page 238: Enter A Data Collection Rate

    User Guide To quickly search through a tag list for the node, enter the initial letters of a tag name in the Filter field. For example, type C*, and then select the Filter button to display a list of tags beginning with C.

  • Page 239: Select Tag Names

    Mobius Chrom 20 System with Flexware Assemblies ® ® If a Phase time of 2 seconds and a Rate time of 10 seconds are assigned, then once collection starts, data will be collected at 2 seconds, 12 seconds, 22 seconds, 32 seconds and every 10 seconds thereafter.

  • Page 240: Enter A Deadband Limit For Data Collection

    User Guide 6. Enter a Deadband Limit for Data Collection Using a deadband limit is a convenient and powerful way to compress data. During times when process values change by insignificant amounts, the deadband limit can improve system performance and decrease the amount of disk space used by historical data files. The deadband limit accomplishes this by controlling how much the current value can deviate from the last recorded value before Historical Collect records the value in the historical data file.

  • Page 241: Suspending A Collection Group

    Mobius Chrom 20 System with Flexware Assemblies ® ® Suspending a Collection Group A collection group can be suspended temporarily if the current data does not need to be viewed, or if the data is taking up too much disk space on the system. To suspend a group, select the group in the Historical Assign window.

  • Page 242: Saving Changes To Historical Assign Configuration

    User Guide Saving Changes to Historical Assign Configuration To save changes to the Historical Assign program, two separate operations are required. 1. Select Save from the File menu. This operation saves the changes made, to support data collection. 2. Select Save As from the File menu. In the File name field in the Save As window, enter the following path and filename: “C:\millipore\ccpsystem\{SystemID}\ini\hta-cfg.csv”...

  • Page 243: Historical Collect Program

    Mobius Chrom 20 System with Flexware Assemblies ® ® Historical Collect Program Starting the Historical Collect Program Starting the Historical Collect application enables data collection. Historical Collect starts automatically when the system starts up and collects data, as configured in the Historical Assign program.
  • Page 244 User Guide 4. In the Customize Regional Options window, click on the Date tab. 5. In the Short date format field, select or manually enter one of the following supported formats from the drop-down menu or type it into the field: •...
  • Page 245 The vibrant M, Millipore, Mobius, Flexware, Pellicon, Common Control Platform, CCP, Opticap, Millipak and Emprove are trade- marks of Merck KGaA, Darmstadt, Germany and its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.

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