Table of Contents
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1 Intr
oduction to the CellASIC
Microfluidic System
1.1 Overview
The CellASIC
with media flow, temperature control, and gas environment control. A selection of
proprietary microfluidic plate designs allows cells to be captured and exposed to
different conditions via flow channels.
The system can be used with typical inverted microscopes and the microfluidic
plates fit standard multiwell plate stage adapters. The CellASIC
System connects to the microfluidic plate via a pneumatic manifold that uses
pressurized air to control flow within the plate. A vacuum seal created between
the manifold and the microfluidic plate ensures that each well is independent and
that flow rates and fluid switching are accurate. Flow and environment control are
managed through a computer software program.
This user guide covers the setup and operation of the CellASIC
System. User guides for the CellASIC
www.sigmaaldrich.com/cellasic.
Figure 1. CellASIC
1.2 Benefits
● The CellASIC
the perfusion, temperature, and gas environment for time-lapse, live-cell
imaging applications.
● The CellASIC
scheduled flow changes without supervision. Customized experiments can
be performed by programming changes to media solutions, flow rates, and
exposure times. The easy-to-use software is flexible and can generate simple
solution-switching protocols or advanced flow profiles.
● The precision laminar flow maintained by the system creates a highly stable
environment for cells. The CellASIC
microliter volumes of fluid and can run for multiple days without refilling.
20202092 04/19
ONIX2 Microfluidic System enables continuous live-cell imaging
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Seal
Manifold
Ready Sealed Error
ONIX2 Microfluidic System setup
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ONIX2 Microfluidic System provides a total solution for controlling
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ONIX2 Software automates the entire experiment, allowing
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ONIX2
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ONIX Microfluidic Plates are available at
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CellASIC
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ONIX2
Microfluidic System
To Microfluidic Plate
ONIX2 Microfluidic System requires only
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1 of 60
ONIX2 Microfluidic
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ONIX2 Microfluidic
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CellASIC
ONIX2 Microfluidic System
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