Merck Millipore CellASIC ONIX2 User Manual page 38

7.7 Menu Items and Advanced Features,
7.7.2
Temperature Calibration, continued
For immersion objectives and air objectives with working distance less
than 2 mm:
NOTE: Objective heater required!
a. Seal the CellASIC
b. Place the manifold and plate onto the microscope stage and set up the
microscope as it will be set up for the cell-based experiment. Correctly position
the condenser and stage, but do not have the objective near the plate.
c. Follow steps 3 and 4 in section above to determine the setpoint and offset
required.
d. Bring the objective close and focus on the features within the viewing region
of the Temperature Calibration Plate. Adjust the setpoint of your objective
heater until the Temperature Calibration Plate is again at the desired
temperature. Stabilization time depends on the objective heater. Use this
objective heater setpoint for your experiment.
Plate Types
You may select the plate types that you
would like to show up on the drop down
menu when creating a new experiment.
20202092 04/19
Tools Menu,
continued
ONIX2 Manifold XT to the Temperature Calibration Plate.
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continued
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CellASIC ONIX2 Microfluidic System
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