Merck Millipore CellASIC ONIX2 User Manual page 20

7.2 Manual Mode Tab,
continued
7.2.2 Environment Control
Allows user to manually set environment control parameters
Environment Control pane
Temperature control can be turned On/Off and the target
setpoint adjusted.
Gas Flow can be turned On/Off, gas A or gas B selected,
and Slow or Fast flow rate selected. Select slow flow rate
if running an experiment in the same gas environment.
Select the fast flow rate when switching to a different gas
environment, so that the new gas environment is achieved
swiftly.
When gas environment control is turned On, consumption of
premixed gas mixture will be approximately 5 mL/min at slow
flow or 50 mL/min at fast flow (e.g., a typical nominal 330
CF tank of premixed gas will last for approximately 6 years at
continuous slow flow or 6 months at fast flow.)
7.2.3 Plate Setup Sequences
Short automated sequences can be run using this feature. When an experiment file
is first created, these sequences are populated with defaults according to the plate
type. Using the default setting specific to each plate is recommended, however,
optimization may still be required depending on the cell type and/or concentration.
Edited sequences are saved as part of the experiment file when the experiment file
is saved and can be repeated by "creating a new experiment based on previous" and
selecting the previously saved experiment. For some plate types, cell loading can be
achieved without using the software and ONIX2 Microfluidic System. For example,
the M04S-03 ONIX microfluidic plates can be gravity-loaded by pipette. In such
cases, this cell loading feature can be skipped.
Plate Setup Sequences pane
Click the Run cell loading sequence, Run liquid
priming sequence, or Run a custom sequence buttons
on the Manual Mode tab to begin execution of that
sequence.
A CANCEL SEQUENCE button appears to allow
cancellation of the currently executing sequence.
The PAUSE/RESUME button allows pausing the
sequence. When paused, all well groups are turned off but
temperature and gas settings are left alone.
Remaining time displays a count down timer of the time
left until the sequence is complete.
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20202092 04/19 CellASIC ONIX2 Microfluidic System
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