Merck Millipore CellASIC ONIX2 User Manual page 15

6.4 CellASIC
NOTE: As an alternative to using premixed tanks and the on-board gas flow
control, you can attach the system to a separate gas mixing source. Simply
attach a gas line flowing the desired gas mixture to the white Luer fitting labeled
GAS on the manifold tubing (
must be at atmospheric pressure and regulated to a flow rate of 3–100 mL/min.
4. Securely attach the vacuum Luer fitting (black), eight pressure Luer filters
(translucent), and gas Luer fitting (white) from the manifold to the Luer fittings
on the CellASIC
The Filter Multiconnector can be plugged into the front panel of the system as
shown in the figure below. Magnets and spacing prevent the connector from
being plugged in incorrectly. To disconnect, pull on the tabs on both ends
B
Figure 6. Connecting the Filter Multiconnector
Important: Match each fitting to the corresponding line in order. The black
fitting must be matched to black line labelled VAC. Before starting each
experiment, make sure all eight Luer filters are securely attached to the fittings
and that the Filter Multiconnector is securely pushed all the way into the front
panel until it seats. At the same time, check to see that there is no liquid
contamination in the manifold tubing and/or filters. If contamination is observed,
clean tubing and manifold by running the
cleaning sequence and replace Luer filter(s)
according to Maintenance and Cleaning.
5. If the temperature controlled CellASIC
Manifold XT is being used, you must also
connect the 4-conductor plug from the manifold.
Align the red mark on the top of the plug to the
dot on the front panel and push the plug into
the socket labeled Manifold (Figure 7) on the
front of the system. The plug can be removed
by pulling on the sheath; be sure to grab only
the knurled sheath when pulling to disconnect.
Figure 7. Connecting the manifold plug
20202092 04/19
ONIX2 Microfluidic System Setup,
®
ONIX2 Filter Multiconnector, cat. no. CAX2-AMC00 (Figure 6).
®
in Figure 6). For this setup, the gas mixture
A
B
A
ONIX2
®
11 of 60
continued
Pull on knurled
sheath to
disconnect
CellASIC ONIX2 Microfluidic System
®
.
B