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Summary of Contents for Merck mPAGE Lux
- Page 1 User Guide mPAGE Lux Gel Casting System ® Kit component quantities may vary from photo. SigmaAldrich.com...
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Page 2: Table Of Contents
mPAGE Lux Casting System ® makes polyacrylamide gels that are ready to use in under 2 minutes. mPAGE Lux Gel Casting System Methods ® Components Gel Casting and Curing ... . . 8 mPAGE Lux Curing Station . -
Page 3: Mpage ® Lux Gel Casting System Components
mPAGE Lux Gel Casting System Components ® mPAGE Lux Curing Station ® The mPAGE Lux Curing Station contains a UV LED panel ® designed to polymerize polyacrylamide gels in under 2 minutes. The Curing Station is compatible only with the mPAGE Gel Caster and mPAGE Lux Reagent Kit. -
Page 4: Mpage ® Mini Spacer Plates
mPAGE Mini Spacer Plates mPAGE Lux Bis-Tris Reagent Kit ® ® (sold separately) Specify thickness when ordering. This flexible reagent kit is designed to simplify casting • 0.75 mm (5 plates) or solution preparation. The Bis-Tris gel chemistry • 1.0 mm (5 plates) or allows for crisp separation of proteins at neutral pH. -
Page 5: Workflow
Workflow Casting Single or Multiple Gels Power ON Curing Station Prepare Solutions Assemble Gel Caster Place Gel Caster in Lux Curing Station Cast Gel Repeat until all gels are cured. Cure Gel Assemble Second Gel Caster if curing multiple gels Remove Gel Caster from Lux Curing Station Power OFF Lux Curing Station... -
Page 6: Preparation
Preparation Assemble mPAGE Gel Caster ® Tension mPAGE Gel Caster Base mPAGE ® ® Diagram Clip Caster Frame See diagram (right). Assembly 1. Inspect the gel sealing gasket for mPAGE Mini Spacer Plate ® defects. Place gasket between the alignment tabs on the bottom of the mPAGE ®... -
Page 7: Prepare Gel Solutions
Prepare Gel Solutions Stacking Gel Protect all solutions from light to prevent polymerization. Before casting, bring all solutions The Stacking Solution is ready to use directly to room temperature. Choose a gel percentage to best from the bottle, do not dilute. separate your target protein(s). -
Page 8: Methods
Methods Gel Casting and Curing 3. Open the mPAGE Lux Curing Station door. ® Insert assembled mPAGE Gel Caster into ® 1. Power ON mPAGE Lux Curing Station by pressing ® mPAGE Lux Curing Station by placing the ® . The device will initiate a self-check. After self- Star t Powe r Cancel... -
Page 9: Sample Preparation And Electrophoresis
Sample Preparation and Electrophoresis 8. Close mPAGE Lux Curing Station door. Ensure ® door is completely closed before proceeding to gel The gels prepared with the mPAGE Gel Caster ® curing. are compatible with the mPAGE Mini Gel Tank ® or Bio-RAD Mini-PROTEAN Cells. -
Page 10: Gel Staining
Gel Staining 7. Attach lid and connect leads to appropriate power supply. Proteins can be visualized using any Bis-Tris compatible gel staining method. Refer to manufacturer’s guidelines • When using MOPS-SDS running buffer, gels can for specific gel stains. Bis-Tris compatible gel be run at 200 V. -
Page 11: Transfer
Transfer Proteins can be transferred to PVDF or nitrocellulose blotting membrane after electrophoresis. Gels are compatible with wet transfer (also known as tank transfer), semi-dry transfer, and fast semi-dry transfer. Prepare fresh transfer buffer based on the type of transfer to be performed. See Compatible Transfer Buffers table below. -
Page 12: Troubleshooting
Troubleshooting Basic Issue Cause Resolution Ensure initialization and countdown begins Instrument not started after pressing start Gel did not cure Never open instrument door during curing cycle or is still liquid Cure cycle not completed, LED Operation Fault, contact Tech Service at error message displayed SigmaAldrich.com/TechService Run interrupted,... -
Page 13: Error Codes
Issue Cause Resolution Air pockets form Too much heat generated Run gels at lower voltage. Limit gels to 1-2 gels between gel and glass during electrophoresis per gel tank during electrophoresis during electrophoresis Confirm the correct volume of Resolving Solution and Incorrect reagent dilution Diluent were used when mixing Resolving Gel Solution Same pipette used in... -
Page 14: Faqs
FAQs Can resolving gel solution be stored in mixing tubes? What if run is interrupted accidentally (by opening curing station door or pressing cancel)? Resolving gel solution is stable and can be stored in Mixing tube at 2-8 °C for short periods. Longer If run is interrupted during initialization period, the run term storage introduces a risk of microorganism can be restarted with existing gel. -
Page 15: Product Ordering
Product Ordering Order products at SigmaAldrich.com mPAGE Lux Gel Casting System ® Select gel thickness and power cord. 0.75 mm (10-wells only) Includes Power Cord For Catalogue Number • mPAGE Lux Curing Station, with Power Cord United States LUXCSYS-75M-US ® •... -
Page 16: Mpage ® Gel Caster
Catalogue Catalogue Description Number Description Number mPAGE Gel Caster Immunodetection Devices ® mPAGE Gel Caster, 2pk SNAP id 2.0 Systems ® GCR2 ® Mini, 7.5 cm x 8.4 cm SNAP2MINI mPAGE Combs ® MultiBlot, 4.5 cm x 8.4 cm SNAP2MB3 0.75 mm, 10 wells C75M10W Mini, 7.5 cm x 8.4 cm and... - Page 17 Catalogue Catalogue Description Number Description Number Transfer Membranes and Blotting Paper Blocking, Enhancing and Stripping Reagents Immobilon Blotting Filter Paper 20 G Immunoblot Blocking Reagent 20-200 ® sheet, 7 cm x 8.4 cm IBFP0785C ChemiBLOCKER™ 500 mL 2170 membrane-blocking agent Immobilon -E Blotting Sandwich ®...
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Page 18: Notice
Merck, Millipore, mPAGE, Immobilon, TurboMix, ReadyBlue, EZBlue, EZFluor, ProteoSilver, ChemiBLOCKER, Western Blocker, SNAP id, Re-Blot, and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.
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