Capturing Images; Procedure For Capturing Images - Nikon SMZ25 Instruction Manual

15

Capturing Images

The microscope system can capture images of the sample being observed by attaching a camera such as a DS camera
head to the vertical tube via a TV tube adapter or C-mount adapter.

Procedure for Capturing Images

15.1
Follow the procedure below to capture images. For the camera settings, see the instruction manual for the DS-L3 or DS-U3.
Using the DS-L3
Specify the following items beforehand.
 Folder to save
 Name of the save file (or select Auto.)
 File format and file size of the save file
 Date and destination of the data
(1) Adjust the illumination of the microscope correctly and
the focus of the sample image.
(2) Adjust the mounting orientation of the DS camera head.
For details about attaching the camera head, see [5] Attach
a Camera in Chapter 5, "3.3 Assembly of Other Devices
(Optional)."
Since shifting a sample horizontally causes the image on
the monitor to move horizontally, loosen the attachment
guide fixing screw and adjust the orientation of the camera.
After making the appropriate adjustments, tighten the screw.
(3) Select the camera scene mode suitable for the microscopy type.
(4) Adjust the camera's white balance.
When using diascopic illumination, first capture an image of the top surface of the stage glass. When using episcopic
illumination, first capture an image of any white subject. Then, press the WB button to execute white balance
adjustment. (For fluorescent photomicrography, Nikon recommends adjusting the white balance under normal
bright-field microscopy conditions before capturing images.)
(5) Adjust the position of the sample.
(6) Readjust the focus on the target.
(7) Adjust the image brightness using the camera exposure compensation function.
(8) Press the Pause button on the DS-L3 to check the image.
(9) Save the image if acceptable.
To save the image, press the capture button on the remote controller or DS-L3, or step on the pedal of the AZ-PCR
Photo Release.
Chapter 3
Specific Operations
34
Adjusting the camera orientation
DS camera head
C-mount adapter
Attachment guide
fixing screw
LV-TV TV Tube
Adapter