Olympus STM7 Instructions Manual page 59

How to Use the Illumination Modes
Select the illumination mode ideal for the specimen.
1
Transmitted light illumination
The contour of a specimen can be observed as a silhouette.
2
Reflected light illumination
Permits observation of the surface of a specimen, especially in the case of a nontransparent specimen.
3
Simultaneous use of transmitted and reflected light permits concurrent observation of the contour and surface of the
specimen.
Illumination brightness adjustment
Adjust the transmitted/reflected light illuminations to the intensity level that suits the observer.
Focusing
Make a precise focus adjustment on the microscope.
As focusing is a key to accurate measurement and a cause for all errors. Perform focusing properly in the following procedures.
1
Make interpupillary distance adjustment and diopter adjustment (page 33).
2
Bring the specimen in focus by moving the reflected light arm up and down with the coarse focusing knob on the focusing
unit of the microscope or the FOCUS buttons on the focus controller STM7-MCZ. Adjust focusing more precisely using the
fine focusing knob on the focusing unit of the microscope or using the fine/coarse focusing knob on the focus controller.
3
To ensure the focusing which has been adjusted visually in the above, check for the presence of parallax. While looking
through the eyepiece sleeves, move the eyes forward/backward and toward the left and right. Assuming that the cross hairs
are stationary, if the image moves in the same direction as the eye movement, bring the objective closer to the specimen. If
the image moves in the direction opposite to the eye movement, raise the objective away from the specimen.
} When using the autofocus unit STM7-AF, see "3-9 Autofocus unit (Motorized frame only)" (page 39).
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