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FLEXAR SQ 300 MS
PerkinElmer FLEXAR SQ 300 MS Manuals
Manuals and User Guides for PerkinElmer FLEXAR SQ 300 MS. We have
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PerkinElmer FLEXAR SQ 300 MS manual available for free PDF download: User Manual
PerkinElmer FLEXAR SQ 300 MS User Manual (120 pages)
Chromera Chromatography Data System
Brand:
PerkinElmer
| Category:
Laboratory Equipment
| Size: 25.71 MB
Table of Contents
Table of Contents
5
Introduction
7
Starting
9
Overview
10
Flexar SQ 300 MS Status Leds
11
LED Functionality
11
Starting the SQ 300 MS Detector
12
Checking the Position of the ESI Probe
13
Starting Chromera
15
Configuring Chromera
16
Creating a System Database
16
Creating an LCMS Instrument
17
Setting the Operate Method
21
Auto Tune on the SQ 300 MS Detector
24
About the Samples
24
PKI Tune MIX
24
Calibrants
24
Calibrant Delivery Modes
24
Preparing the Calibration Vials for Auto Tuning
25
Run an Auto Tune
26
Running Auto Tune from the Calibration Vials
27
Running Auto Tune Using the Built-In Syringe Pump
35
Initial Process to Configure an Optimal Tune and Method on the SQ 300 MS Detector
37
Process Overview
38
Setting up a Sample Infusion
39
Creating a Peak Detection Mini-Method
40
Ramping Parameters- Optimizing the Capillary Exit Voltage
48
Creating Methods and Sequences
55
Creating an MS Method
56
Creating a Chromera Method
63
Creating a Chromera Sequence
66
Starting Data Acquisition
69
Preparing for an Analysis
70
Equilibrate the System
71
Running a Sequence
73
Analyze Results in Post Run
77
Viewing the Results in Post Run
78
Importing Chromera Data and Methods
82
Using the Data Selector
85
Searching a Library to Identify Unknowns
87
Viewing Spectra Results in the SQ 300 MS Driver Window
90
About the Total Ion Chromatogram (TIC)
92
Creating an Extracted Ion Chromatogram (EIC)
93
Using the EIC Dialog
93
Creating a Base Ion Chromatogram (BIC)
95
Processing of Ion Chromatograms
97
Displaying Statistics
98
About Right Mouse Click Menus
98
Baseline Calculation
98
Setting the Chromatogram Noise Calculation Preferences
99
Chromatogram Threshold
99
Chromatogram Smoothing
100
Chromatogram Peak Detection
100
Manual Peak Detection
100
Automatic Peak Detection
101
Evaluating Mass Spectra
105
Evaluating Acquired Mass Spectra
106
Creating an Average Mass Spectrum
108
Using the Left Mouse Button Command
108
Using the Generate Mass Spectrum Dialog
108
Creating an EIC and BIC from a Mass Spectrum
110
Processing of Mass Spectra
112
Freezing and Thawing Mass Spectra
112
Zooming in
112
Displaying Statistics
113
Using Right Mouse Click Menus
113
Baseline Calculations
113
Setting Spectrum Noise Calculation Preferences
114
Setting the Mass Spectrum Threshold
115
Mass Spectrum Smoothing
115
Mass Spectrum Peak Detection
116
Manual Peak Detection
116
Automatic Peak Detection
116
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