PerkinElmer LAMBDA 650 Hardware Manual
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PerkinElmer LAMBDA 650 Hardware Manual

Uv-vis spectroscopy
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UV-VIS SPECTROSCOPY
L
650/850/950
AMBDA
Hardware Guide

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Summary of Contents for PerkinElmer LAMBDA 650

  • Page 1 UV-VIS SPECTROSCOPY 650/850/950 AMBDA Hardware Guide...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    Lambda 650/850/950 Hardware Guide . 3 Table of Contents Table of Contents ................... 3 Introduction..................5 Overview ....................6 Conventions Used in this Manual.............. 7 Notes, Cautions and Warnings ............7 Safety Practices ................11 Overview ..................... 12 Precautions..................13 General Operating Conditions ..............14 Environmental Conditions..............
  • Page 4 4 . Lambda 650/850/950 Hardware Guide Removing the Detector Unit ............67 Accessory Connector Panel.............. 69 Specific Accessory Installation Overviews ......... 70 Pin Configuration................71...

  • Page 5: Introduction

    Introduction...

  • Page 6: Overview

    6 . Introduction Overview This hardware guide covers the operating instructions for the Lambda 650, Lambda 850 and Lambda 950 instruments plus those maintenance routines that do not necessarily require a PerkinElmer Service Engineer. Responsible Body: Individual or body responsible for the use and maintenance of equipment NOTE: and for ensuring that OPERATORS are adequately trained.

  • Page 7: Conventions Used In This Manual

    UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used to show that you have to press two keys at the same time, for example, ALT+F. All eight digit numbers are PerkinElmer part numbers unless stated otherwise. Notes, Cautions and Warnings Three terms, in the following standard formats, are also used to highlight special circumstances and warnings.
  • Page 8 8 . Introduction We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one. Caution (Achtung) Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um einen Geräteschaden zu vermeiden.
  • Page 9 Lambda 650/850/950 Hardware Guide . 9 We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING Warning (Warnung) Bedeutet, daß...
  • Page 10 10 . Introduction...

  • Page 11: Safety Practices

    Safety Practices...

  • Page 12: Overview

    This advice is intended to supplement, not supersede, the normal safety codes in the user's country. It is also a supplement to the PerkinElmer standard Safety and Health Policy. The information provided does not cover every safety procedure that should be practiced.

  • Page 13: Precautions

    • Do not operate the instrument with any covers or parts removed. • Servicing should be carried out only by a PerkinElmer service representative or similarly authorized and trained person. • Disconnect the instrument from all voltage sources before opening it for any adjustment, replacement, maintenance, or repair.

  • Page 14: General Operating Conditions

    If any adjustment, maintenance and repair of the opened instrument is necessary, this must be done by a skilled person who is aware of the hazard involved. Whenever it is likely that the Lambda 650/850/950 is unsafe, make it inoperative. The Lambda 650/850/950 may be unsafe if it: •...

  • Page 15: Environmental Conditions

    Lambda 650/850/950 Hardware Guide . 15 Environmental Conditions The instrument has been designed to be safe under the following conditions: • Indoor use • Altitude up to 2000 m • Ambient temperatures of 5 C to 40 • A maximum ambient relative humidity of 80% for temperatures up to 31...
  • Page 16 16 . Safety Practices Toxic Fumes If you are working with volatile solvents or toxic substances, you must provide an efficient laboratory ventilation system to remove vapors that may be produced when you are performing analyses. Waste Disposal Waste containers may contain corrosive or organic solutions and small amounts of the substances that were analyzed.

  • Page 17: Electrical Safety

    To ensure satisfactory and safe operation of the instrument, it is essential that the green/yellow lead of the line power cord is connected to true electrical earth (ground). If any part of the instrument is not installed by a PerkinElmer service representative, make sure that the line power plug is wired correctly:...

  • Page 18: Emc Compliance

    18 . Safety Practices EMC Compliance EC directive This product complies with the minimum immunity requirements of IEC 61326 and has been tested to the relevant parts of the following standards: IEC 61000-4-2 IEC 61000-4-3 IEC 61000-4-4 IEC 61000-4-5 IEC 61000-4-6 IEC 61000-4-11 This product complies with EN 55011 Group 1 Class A and with IEC 61000-3-2 and 61000-3-3.

  • Page 19: Warning Labels

    Lambda 650/850/950 Hardware Guide . 19 Warning Labels When this label is attached to an instrument it means refer to the manual. WARNING The following electrical warnings are shown on the rear of the instrument, as identified in Figure 1:...
  • Page 20 20 . Safety Practices The following warnings are shown on the inside of the lamp compartment, as identified in Figure 2: Lamp compartment Figure 2 Position of the lamp compartment...

  • Page 21: Fuses

    A click is heard as each lug snaps into place. If you use the correct fuses but the instrument still does not work correctly, or the fuses NOTE: blow repeatedly, contact your PerkinElmer office or representative.

  • Page 22: Moving The Instrument

    CAUTION PerkinElmer service representative for guidance or help. If you are only moving the instrument within the laboratory or to a nearby laboratory then use four people and lift the instrument using the handholds, as shown in Figure 5.

  • Page 23: System Description

    System Description...

  • Page 24: Overview

    24 . System Description Overview The Lambda 650, 850 and 950 are versatile spectrometers operating in the ultraviolet visible (UV/Vis) spectral ranges. Additionally, the Lambda 950 operates in the near infrared region. The spectrometer features a double-beam, double monochromator, ratio recording optical system.

  • Page 25: Optical System

    Lambda 650/850/950 Hardware Guide . 25 Optical System The Lambda 650/850/950 Spectrometer features an all-reflecting, double-monochromator optical system. The optical components are coated with silica for durability. Holographic gratings are used in each monochromator for the UV/Vis range and the NIR range.
  • Page 26 26 . System Description For operation in the ultraviolet (UV) range, mirror M1 is raised to permit radiation from the deuterium lamp to strike source mirror M2. Source change is automatic during monochromator slewing. Radiation from the respective source lamp is reflected from mirror M2 via mirror M3 through an optical filter on the filter wheel assembly (FW) to mirror M4.
  • Page 27 There are internal attenuators for the Lambda 850 and 950 which can be swung individually into the sample beam (SBA) and the reference beam (RBA). The attenuators have the values: 0%, 1%, 10% and 100%. The Lambda 650 requires manual attenuators to be placed as required (L6020277 – 10% attenuator, L6020276 – 1% attenuator).

  • Page 28: Specifications

    28 . System Description Minimum Performance Specifications Specification Lambda 950 Lambda 850 Lambda 650 Principle Double beam, double Double beam, double Double beam, double monochromator, ratio recording monochromator, ratio monochromator, ratio UV/Vis/NIR spectrophotometer recording UV/Vis recording UV/Vis with microcomputer spectrophotometer with...
  • Page 29 Lambda 650/850/950 Hardware Guide . 29 Specification Lambda 950 Lambda 850 Lambda 650 Stray Light At 200 nm (12 g/l KCl > 2 A > 2 A > 2 A USP/DAP method) At 220 nm (10 g/l NaI ≤0.00007 %T ≤0.00007 %T...
  • Page 30 30 . System Description Specification Lambda 950 Lambda 850 Lambda 650 Photometric Linearity (Addition of filters UV/Vis at 546.1 nm, Slit 2 nm, 1-sec. integration time) At 1.0 A +/- 0.006 A +/- 0.006 A At 2.0 A +/- 0.017 A +/- 0.017 A...
  • Page 31 Lambda 650/850/950 Hardware Guide . 31 Specification Lambda 950 Lambda 850 Lambda 650 Photometric Noise 0 A and 190 nm ≤0.00010 A ≤0.00010 A ≤0.00010 A 0 A and 500 nm ≤0.00005 A ≤0.00005 A ≤0.00005 A 2 A and 500 nm ≤0.00020 A...
  • Page 32 32 . System Description...

  • Page 33: Lambda 650 Installation

    Lambda 650 Installation...

  • Page 34: Overview

    34 . Lambda 650 Installation Overview Only the Lambda 650 is designed to be customer installed. CAUTION For the Lambda 850 or 950 please arrange for installation with your local PerkinElmer Service Representative. Requirements For maximum stability and minimum maintenance, observe the following requirements when choosing where to site the instrument: •...

  • Page 35: Unpacking

    Lambda 650/850/950 Hardware Guide . 35 Unpacking Take great care when installing your Lambda 650, and follow the procedures described in this manual. If you require assistance, contact CAUTION your local PerkinElmer Service Representative. The instrument weighs about 76 kg. Four people are needed to lift the instrument.
  • Page 36 L6020277 2 10% manual attenuators L6020276 2 1% manual attenuators The two manual attenuators are only supplied with a Lambda 650. NOTE: 3. Examine the components for any signs of damage in shipment: • Check the entire outer cabinet of the spectrometer for damage, and make sure that terminals, fuse holders, etc.

  • Page 37: Connecting To The Line Power Supply

    Lambda 650/850/950 Hardware Guide . 37 Connecting to the Line Power Supply Electrical Hazard To prevent potential injury to yourself and damage to the instrument, first make any electrical connections in the system before connecting to the line power supply.

  • Page 38: Connecting The System Components

    38 . Lambda 650 Installation Connecting the System Components Electrical Hazard To prevent potential injury to yourself and damage to the instrument, switch OFF all instruments in the system and disconnect them from the WARNING line power supply before you alter, or make any new electrical connections.

  • Page 39: Setting Up The Instrument In The Software

    Lambda 650/850/950 Hardware Guide . 39 Setting up the Instrument in the Software Before you can use your instrument with UV WinLab software, you must set up the instrument in the UV WinLab Explorer. If your instrument is Service installed you should not need to do this.
  • Page 40 40 . Lambda 650 Installation 5. Select the instrument type to add from the drop-down list, and if required, select Make this the default instrument. 6. Click Next. 7. From the drop-down list, select the Port the instrument is connected to.
  • Page 41 These can be selected if you are going to use them, even if they are not currently installed. Manual Attenuators are only available for the Lambda 650, the Lambda 850 and 950 have NOTE: automatic attenuators. The Common Beam Depolarizer requires a firmware command to be enabled.

  • Page 42: Calibrating The Instrument

    42 . Lambda 650 Installation Calibrating the Instrument Once the instrument is set up in the software you will need to calibrate it. Make sure that the instrument has been switched on for at least an hour before calibrating it.
  • Page 43 Lambda 650/850/950 Hardware Guide . 43 • Manual set 2 peaks enables you to change the offset and factor by specifying the old and new values of 2 well known peaks by entering values of Old Peak 1, New Peak 1, Old Peak 2 and New Peak 2.
  • Page 44 44 . Lambda 650 Installation...

  • Page 45: Using The Instrument

    Using the Instrument...

  • Page 46: Startup Procedure

    46 . Using the Instrument Startup procedure If you have purchased accessories you may want to install these first before commencing NOTE: with the Startup procedure. An overview of accessories is available on page 70, see also the specific documentation for the accessory. Do not turn power to the instrument on and off quickly as this may damage the power supply.

  • Page 47: The Single Cell Holder

    Lambda 650/850/950 Hardware Guide . 47 The Single Cell Holder Description There are two single-cell holders provided with the instrument, one for the sample beam and one for the reference beam. The single-cell holders are mounted on a plinth to bring them in line with the radiation beam.

  • Page 48: Aligning The Single-Cell Holder

    Sample Holder and Plinth Tube Ports Figure 14 Lambda 650/850/950 Sample Compartment Baseplate 5. Move the milled posts a little to locate the threaded holes in the baseplate, and then tighten the milled posts. You can lead tubes and connecting cables for accessories into the sample compartment through either the tube ports, located at the front of the sample compartment, or the opening located at the left rear of the sample compartment under the cover plate.

  • Page 49: Fine Alignment

    Lambda 650/850/950 Hardware Guide . 49 7. By holding a piece of matt white paper behind each cell holder, visually examine the light spot to see that the radiation beam is passing through the cell sample area. Diffraction patterns become apparent if the radiation beam impinges on the cell wall.

  • Page 50: Minimum Volume Applications

    When the cell holder has been aligned once, you can take it out and reinstall it without NOTE: aligning it again. Minimum Volume Applications To measure minimum sample volumes, use microcells (offered by PerkinElmer). The minimum sample volume required is a function of the cell internal width or volume and is specified below.
  • Page 51 Lambda 650/850/950 Hardware Guide . 51 Cell Type Cell Pathlength Minimum Part Internal Volume Number Width Required Height of 2 mm 1 cm 150 µL B0079404 liquid slightly (pair) more than height of 4 mm 1 cm 300 µL B0079402...

  • Page 52: Instrument Purging (Lambda 850 And 950 Only)

    52 . Using the Instrument Instrument Purging (Lambda 850 and 950 only) Always purge the instrument with an inert gas such as Nitrogen. Never use a flammable gas such as Hydrogen. WARNING Oxygen absorbs radiation in the UV range below 190 nm, while water vapor absorbs radiation in the NIR range between 1350 nm and 1450 nm, 1850 nm and 1950 nm, and also between 2520 nm and 3000 nm.
  • Page 53 A Nitrogen Purge Accessory Kit, comprising of a filter and a flow controller with flowmeter, is NOTE: offered as an option. With this kit you can control the gas flow more accurately. The Lambda 650 does not have this purge facility. NOTE:...
  • Page 54 54 . Using the Instrument...

  • Page 55: Maintenance

    Maintenance...

  • Page 56: Overview

    You should perform only the maintenance procedures described in this chapter. If additional maintenance is required, contact a PerkinElmer Service Engineer. Before using any cleaning or decontamination methods except those specified here, users should check with PerkinElmer that the proposed CAUTION method will not damage the equipment.

  • Page 57: Daily Checks

    Lambda 650/850/950 Hardware Guide . 57 Daily Checks The instrument is constructed with high quality components and requires little maintenance other than to keep it clean and free of dust. To protect the optical system from dust and fumes, you should keep the sample compartment cover closed except for when you are carrying out work in the compartment.

  • Page 58: Sample Compartment Windows

    58 . Maintenance Drain Holes Figure 17 Drain holes in standard sample compartment baseplate 1. First remove the cell holder or other sample handling accessory from the sample compartment. 2. Using a soft cloth and mild laboratory detergent solution, lightly scrub away all foreign material.
  • Page 59 Lambda 650/850/950 Hardware Guide . 59 any optical component. Optical faults of a minor nature, scratches, lint, finger marks, etc. on the optical surfaces can easily introduce substantial analytical errors. You should observe the following list of cell handling rules to prevent analytical errors and to achieve utmost precision: •...

  • Page 60: Replacing A Lamp

    60 . Maintenance Replacing a Lamp If a lamp failure warning message is displayed, or you are seeing excessive noise on the baseline, you can replace the lamps as follows. Electrical Hazard High voltages are present at the lamp connectors in the lamp compartment –...

  • Page 61: Halogen Lamp Replacement

    Lambda 650/850/950 Hardware Guide . 61 Halogen Lamp Replacement If the lamp burns out, or if the bulb becomes blackened after prolonged use, you should replace the lamp. Replacement lamp assemblies (B0114620) are provided complete with pre-aligned mounts. Figure 19 Pre-aligned Halogen Lamp 1.

  • Page 62: Deuterium Lamp Replacement

    62 . Maintenance Halogen lamp connector Halogen lamp assembly Thumbscrew (halogen lamp) Figure 21 Removing the halogen lamp connector 5. Remove the halogen lamp assembly from the bracket by slackening the thumbscrew and pulling the lamp mount vertically upward. Save the thumbscrew for use with the new lamp assembly. 6.
  • Page 63 Lambda 650/850/950 Hardware Guide . 63 An operating hours counter is incorporated in the red deuterium lamp lead. By means of a NOTE: gap between the two display bars it is possible to read off the number of hours that the lamp has been in operation.

  • Page 64: Replacement Parts

    64 . Maintenance Replacement Parts Supplies, accessories, and replacement parts can be ordered directly from PerkinElmer. e-ssentials, PerkinElmer’s catalog service, offers a full selection of high-quality ultraviolet essentials.perkinelmer.com supplies, see Quantity Item Part Number Pack of 10 fuses 3.15 A time delay...

  • Page 65: Accessory Installation

    NOTE: need an accessory printed circuit board (PCB) fitted in the connector panel. The accessory PCB will be installed by a PerkinElmer service engineer. This section of the manual details changes that may need to be made to the basic spectrometer before installing an accessory and an overview of the installation required for some of the specific accessories.
  • Page 66 66 . Maintenance Removing the Sample Compartment Windows Each window has a magnetic frame and can be carefully removed by hand. Replace the window in its original position. Removing the Sample Compartment Baseplate 1. With the sample compartment cover open or removed, undo the four thumbscrews shown in Figure 24.

  • Page 67: Removing The Detector Unit

    Lambda 650/850/950 Hardware Guide . 67 Retaining Screw Locating Slot Retaining Screws Retaining Screw Locating Hole Figure 25 Four retaining screws in the sample compartment 2. Lift up the sample compartment off the locating pins and remove it from the spectrometer.
  • Page 68 Make sure you screw down the detector unit as this creates the earth bonding for elctrical safety. WARNING Details of the installation procedure for a sphere or Universal Reflectance Accessory (URA) Lambda 650/850/950 User Manuals are given in the relevant manual on the...

  • Page 69: Accessory Connector Panel

    Figure 28 Connector Panel for Accessories The insulation of externally connected accessories should meet the requirements of IEC NOTE: 61010-1 or IEC 60950. Peltier Cell Holder Peltier Cell Changer Automatic Cell Changer External Printer Temperature Sensor Sipper Figure 29 Possible accessories for a Lambda 650/850/950...

  • Page 70: Specific Accessory Installation Overviews

    70 . Maintenance Specific Accessory Installation Overviews The Sphere and URA accessories are covered in separate manuals on the NOTE: Lambda 650/850/950 User Manuals Automatic Cell Changers Connector on Spectrometer: 15-pin connector Installation: See cell changer manual Cables: Lead through bottom of spectrometer housing Tubes: Lead through bottom of spectrometer housing Sample Compartment Cover:...

  • Page 71: Pin Configuration

    Figure 30 Sipper, electrical connection Pin Configuration There are two 15-pin connectors fitted to the connector panel of the Lambda 650/850/950 spectrometer to connect accessories. The connectors are identically configured so that the accessories can be connected to any of them. The pin numbering is shown in Figure 31, and the configuration is given in the following table.
  • Page 72 72 . Maintenance...

This manual is also suitable for:

Lambda 850Lambda 950

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