3D Histech PANNORAMIC SCAN RX User Manual page 98

PANNORAMIC® SCAN RX 1.0
Set the correct focus value of the desired preview position by clicking SetI1, SetI2, or SetI3.
If you want to go back to any of the previously set focus points, click the appropriate
button. To delete the stored values, click Clear Values.
The software calculates a coarse focus level from the three values determined this way as a
basis for the fine focusing routine. You can only define the start parameter here. This will,
however, speed up the focusing process.
If you deselect the Use fine focus checkbox, the software immediately starts scanning
based on the interpolated values, without pre-fine-focusing.
To define focus limits, select Limits of the range. Set the upper and lower limits by selecting
the desired value in the selection boxes. By using ± Range, the Lower limit value is set to -32
of the current focus value and the Upper limit value is set to +32 of the current focus value.
This is useful if Auto Focus does not find the focus on the specimen, but on the coverslip.
Also, this makes the focusing sequence faster.
For manual focusing define the manual focus step size by adjusting the Step size slider and
use the two arrow buttons to focus manually. This is useful when you are interested in an
area that remained blurry with Auto Focus (for example, the specimen is thicker in that
area). This does not influence the way slides are digitized in Auto focus mode.
5.2.2.3 Exposure Indicator
Exposure time for the live image can be calculated by using the Calculated Exposure time
function . It is available to switch between microsecond, millisecond, and second units
for the reading of exposure time.
Figure 83. Window of Exposure Indicator in Fluorescence scan mode
Select the types of filters you want to use during scanning. You can select the filter types
and the number of channels to be used. The values in the EXC column define the excitation
of the Lumencor illumination, in TW define exposure time weight, and in the DG the
amount of digital gain is displayed. It is also available for you to enable Extended Focus ( EF)
and/or Z-stack (ZS) functions in this field.
MAY 13, 2021
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Figure 82. Focus indicator panel
SCANNING MODES AND SETTINGS
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