3D Histech PANNORAMIC SCAN RX User Manual page 63

PANNORAMIC® SCAN RX 1.0
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Z-Stack: For Extended focus and Z-Stack scanning methods, you can determine the
desired number of focus levels with the Levels selection box. The current focus value is
the initial level. If you set 5 for example, then the initial level, plus four (two focus levels
above and below the FOVs) are captured. Therefore, it is advised to choose an odd
number for the focus levels. Separate layers are created and stored this way and can be
opened with Viewer applications. The Z-Stack slide contains the extended focus layers.
Step size determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are: Levels: 7; Step size: 5. The number of iterations the Deconvolution
algorithm is run can be determined in the Iteration number for deconvolution: value box.
Notice:
The purpose of deconvolution is to produce a sharper image, but the use of a deconvolution
algorithm can significantly increase the time that takes to create a digital slide.
The capacity of the required memory for deconvolution is minimum 96GB RAM.
Scanning Settings
Includes the Stop and go scanning mode by which the slide tray stands still during recording the
FOV, then it moves on so that the scanner can record the next FOV.
Use Color matrix: Select an applicable preset color profile to be used for Brightfield scanning
from the drop-down menu. Settings according to the profile has effect on the color set, color
temperature, and gamma value of the scanned image. Separate color matrix can be defined and
used for each profile and scanning.
Preset profiles are the following:
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Calibrated Linear: Color scheme is defined by calibration (Gamma value is not included).
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sRGB standard: Color scheme is defined by calibration (standard Gamma value -2.2- is
applied).
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sRGB standard microscope-like: Color scheme is defined by calibration (standard Gamma
value / White Point: D50).
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Legacy: A Color scheme calibrated for the previous software version is applied.
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Custom: Color profile set in Service is applicable if needed.
MAY 13, 2021
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Figure 40. Focus level settings
SCANNING MODES AND SETTINGS
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