3D Histech PANNORAMIC SCAN RX User Manual page 84

PANNORAMIC® SCAN RX 1.0
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Custom scanmap: The scanmap drawn on the preview image is used for scanning.
If the Use selection box restriction function is activated, only the area inside the box is
scanned. It is useful if you only want to scan the area that is equivalent with the size of the
coverslip, so external specks will not be scanned.
Multilayer mode
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Single layer mode: If none of the filters are selected in the Filters table for multilayer
scanning, this mode is active and can be selected, and Multilayer mode turns inactive.
Extended focus: By choosing the Extended focus option, the software selects the
sharpest image from each focus level for each image field and combines them into one
single image. Therefore, those parts that were blurry in auto/manual focus will also be
sharp. This method guarantees the maximum depth of sharpness; however, it reduces
digitization speed.
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Z-Stack: For Extended focus and Z-Stack scanning methods, you can determine the
desired number of focus levels with the Levels selection box. The current focus value is
the initial level. If you set 5 for example, then the initial level, plus four (two focus levels
above and below the FOVs) are captured. Therefore, it is advised to choose an odd
number for the focus levels. Separate layers are created and stored this way and can be
opened with Viewer applications. The Z-Stack slide contains the extended focus layers.
Step size determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are: Levels: 7; Step size: 5.
The number of iterations the Deconvolution algorithm is run can be determined in the
Iteration number for deconvolution: value box.
Notice:
The purpose of deconvolution is to produce a sharper image, but the use of a deconvolution
algorithm can significantly increase the time that takes to create a digital slide.
MAY 13, 2021
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Figure 66. Multilayer mode settings
SCANNING MODES AND SETTINGS
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