3D Histech PANNORAMIC SCAN RX User Manual page 136

PANNORAMIC® SCAN RX 1.0
4. Find the focal plane, then use Go within range and + -Range to set how many images the
Scanner should take in the Z-direction. (The distance between the two images in Z-
direction is 0.2 μm). Under the Output folder field, select the folder where the images are
saved (it is a good idea to create a new folder for each recorded image sequence), and
use the Prefix of output filename option to add a prefix to each image in the sequence.
Once you have finished, click Generate PSF button and the Scanner will start recording
the image sequence.
5. As the last step of setting the deconvolution process, load generated PSF results into the
software. Select the appropriate PSF output for each of the filters in Settings mode under
the Browse tab of the PSF settings. Enter the number of images in the series that the PSF
consist of, then select the folder under Folder name into which the PSF results are saved.
Click Save to accept modifications.
6. Turn on deconvolution function by selecting D on the Filter settings panel in Microscope
mode. This way scanning can be performed with the correct parameter settings.
Notice:
Deconvolution can be performed in Extended focus or Z-Stack scanning modes only.
MAY 13, 2021
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
POINT SPREAD FUNCTION
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