Performance Check: Calibration / Verification - Elitech FreezePoint 6000 Series User Manual

Installation, Operation, and Performance Qualification

Performance Check: Calibration / Verification

There are two modes of operation of the FreezePoint
• Calibrating the system with distilled water and one or two calibration standards.
• Measuring the osmolality of a sample.
The calibration of the FreezePoint
multi-pass measurements.
This means that the complete calibration (2 or 3 point) is one process. After all required points are
measured the results will be displayed and can be accepted, partly disabled or completely discarded.
General Mode of Operation
Method:
1. Choose the operation that should be performed (measure or calibration)
2. Fill a measuring vessel with the appropriate volume of the sample into a dry and clean measuring
vessel. Make certain that there are no air bubbles in the sample.
◊ Model 6000 / Model 6000P - 15μL
◊ Model 6000S / Model 6000SP - 50μL
3. Place the measuring vessel securely onto the measuring vessel holder (thermistor probe). There will
be a slight resistance and you will hear a click when the vessel is in place.
4. Push the vessel holder down into the measuring position. There will be a noticeable resistance. The
measuring vessel is immediately cooled by the lower cooling system.
5. A progress bar is showing the cooling process. When the under-cooling temperature is reached, the
crystallization is automatically initiated by the injection of ice crystals and a signal will be heard.
6. The resulting ice formation causes the temperature to rise to the freezing point of the solution. A
freezing plateau then forms that is measured by the thermistor probe. The osmolality is recorded,
saved and presented on the digital display in mOsmol/kg.
7. After the measurement process is finished, regardless of whether it was successful or produced an
error message, the elevator will be raised, the measurement system will be automatically switched to
standby and the system is ready for the next measurement.
8. After each measurement, the thermistor probe should be thoroughly cleaned with a soft dry paper
tissue and a new measuring vessel is to be used for each measurement.
COMMENTS
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Date ............................
Completed by ..............................................
Date ............................
Verified by ..............................................
Freezing Point Osmometer:
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Freezing Point Osmometer consists of two or three single-pass or
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