D • Faq; How Do I Connect To The Dionex As-Dv; Why Are The Retention Times Moving; How Do I Adjust Retention Times - Thermo Scientific Dionex Easion Operator's Manual

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D.1 How do I connect to the Dionex AS-DV?
For instructions on how to connect the Dionex Easion to the Dionex AS-DV, refer
to the autosampler operator's manual.
D.2 Why are the retention times moving?
Retention times can shift if the pump flow is erratic, or if the column or eluent is
contaminated.
To troubleshoot pump flow issues, see
If you suspect eluent contamination: Remake the eluent, using concentrated
eluent and ASTM Type I (18.2 megohm-cm) filtered and deionized water (see
Section
3.4).
If you suspect column contamination: Clean the column as instructed in the
column manual.
Note that changes in ambient temperature will have an impact on peak retention
times. The effect varies with the type of column installed, but it is generally more
pronounced for certain cation analyses. The magnitude of the effect varies with
the analyte and is proportional to the temperature changes that the column is
exposed to.
D.3 How do I adjust retention times?
Retention times are calculated during calibration. The Use Recently Detected
Retention Times parameter in the Chromeleon Processing Method Editor (on the
General tab page) can be used to compensate for some causes of retention time
drift; for example, evaporation of volatile components in premixed solvents or an
aging column. For more information, refer to the Chromeleon Help.
Doc. 155028-01 08/20
Section
4.6.
D • FAQ
99

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