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Dionex Easion Operator's Manual
•
Liquid sample is injected into the eluent stream either manually or
automatically (if an autosampler is installed).
•
The pump forces the eluent and sample through a separator column (a
chemically-inert tube packed with a polymeric resin).
2. Separation
•
As the eluent and sample are pumped through the separator column, the
sample ions are separated. In the Dionex Easion, the mode of separation is
called ion exchange. This mode is based on the premise that different
sample ions migrate through the IC column at different rates, depending
upon their interactions with the ion exchange sites.
3. Detection
•
After the eluent and sample ions leave the column, they flow through a
suppressor that selectively enhances detection of the sample ions while
suppressing the conductivity of the eluent.
•
A conductivity cell monitors and measures the electrical conductance of
the sample ions as they emerge from the suppressor and produces a signal
based on a chemical or physical property of the analyte.
4. Data Analysis
•
The conductivity cell transmits the signal to a PC running
chromatography software.
•
The chromatography software analyzes the data by comparing the sample
peaks in a chromatogram to those produced from a standard solution. The
software identifies the ions based on retention time, and quantifies each
analyte by integrating the peak area or peak height. The results are
displayed as a chromatogram, with the concentrations of ionic analytes
automatically determined and tabulated.
104
NOTE For a glossary of chromatography terms, see
Appendix
F.
Doc. 155028-01 08/20
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