Thermo Scientific HistoStar Operator's Manual
  1. Manuals
  2. Brands
  3. Thermo Scientific Manuals
  4. Laboratory Equipment
  5. HistoStar
  6. Operator's manual
Thermo Scientific HistoStar Operator's Manual

Thermo Scientific HistoStar Operator's Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Thermo Scientific
HistoStar™
Operator Guide
A81010100 Issue 2

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the HistoStar and is the answer not in the manual?

Questions and answers

Related Manuals for Thermo Scientific HistoStar

Summary of Contents for Thermo Scientific HistoStar

  • Page 1 Thermo Scientific HistoStar™ Operator Guide A81010100 Issue 2...
  • Page 3 Do not use this device in close proximity to sources of strong electromagnetic radiation (eg: unshielded intentional RF sources) as these may interfere with the proper operation. The Thermo Scientific HistoStar™ is referred to throughout this, and other supporting documents as the HistoStar . Para Trimmer® is a registered trademark of Thermo Fisher Scientific.
  • Page 5 Symbols The following symbols and conventions may be used throughout this document and on the instrument: This symbol is used on the equipment, or in a document, to indicate that instructions must be followed for safe and correct operation. If this symbol appears on the instrument, always refer to the operator guide.

  • Page 7: Table Of Contents

    Table of Contents Safety Information ..............................1 How To Use This Guide ............................5 Chapter 1 - Introducing HistoStar ........................7 Introduction to HistoStar..........................7 Compatibility ..............................8 Identification of Parts ............................9 System Interfacing ............................13 System Specification ............................15 Chapter 2 - Basic Operation ..........................
  • Page 8 HistoStar Operator Guide A81010100 Issue 2 Touch Screen Display ..........................77 Wax Tank and Dispense ..........................78 Forceps Block ............................80 Hot Spot ..............................81 Tissue Storage Tank........................... 82 Temperature Sensors..........................83 Base Mould Storage ........................... 84 Cold Spot ..............................85 Cold Plate ..............................

  • Page 9: Safety Information

    Safety Information Introduction Thermo Fisher Scientific products are designed for convenient and reliable service; however, incorrect actions by a user may damage the equipment, or cause a hazard to health. The following sections contain important information for the safe setup and use of the instrument. All users must read and understand the following sections before using the instrument.
  • Page 10 HistoStar Operator Guide A81010100 Issue 2 Chemical Safety HE INTRODUCTION OF CHEMICALS CREATES POTENTIAL HAZARDS AND HERMO ISHER CIENTIFIC HAS ADOPTED THE FOLLOWING POSITION WITH REGARD TO THE SUBJECT OF VOLATILE CHEMICALS USED IN MEDICAL LABORATORIES • Non-specified chemicals are used in the instrument at the customers own risk.
  • Page 11 Safety Information Warranty Statement We at Thermo Fisher Scientific are proud of our quality, reliability and of our after-sales service. We continuously strive to improve our service to our customers. Please ask your distributor or Thermo Fisher Scientific representative about Service Contracts which can keep your purchase in peak condition for many years to come.
  • Page 12 HistoStar Operator Guide A81010100 Issue 2 Product Return Safety Declaration...

  • Page 13: How To Use This Guide

    Chapter 3 - Setup This chapter explains the procedures used to set-up the HistoStar . The information contained in this chapter also explains how users can set their own preferences to help HistoStar to fit into their workflow more efficiently.

  • Page 15: Chapter 1 - Introducing Histostar

    Chapter 1 - Introducing HistoStar Introduction to HistoStar The HistoStar is an ergonomically designed, precision engineered, tissue embedding system, comprising two temperature controlled modules. The Embedding Module includes a base mould storage compartment, a wax reservoir, a tissue storage tank, a wax dispenser and a Para Trimmer.

  • Page 16: Compatibility

    HistoStar Operator Guide A81010100 Issue 2 Compatibility The HistoStar is designed to be compatible with most tissue cassettes and moulds, including Super Mega Cassettes and SecureSette. It is also designed to be compatible with most brands of histology grade paraffin wax - see Appendix D -...

  • Page 17: Identification Of Parts

    Chapter 1 - Introducing HistoStar Identification of Parts Embedding Module Heated Module - Front View 1 - Wax Tank Cover 7 - Cold Spot 2 - Base Mould Storage Compartment 8 - Dispense Lever (Serial Number Label is inside) 9 - Wax Dispense Flow Control...
  • Page 18 HistoStar Operator Guide A81010100 Issue 2 Heated Module - Rear View 13 - Footswitch Connector 16 - Power Switch 14 - Cold Plate Timer Connector 17 - Mains Power Connector 15 - Fuse Drawer 18 - Rating Plate...
  • Page 19 Chapter 1 - Introducing HistoStar Heated Module - Underside View 19 - Dispense Lever Position Adjustment Knob 21 - Tissue Storage Tank Drain Valve Lever 20 - Heated Forceps Connector 22 - Tissue Storage Tank Drain...
  • Page 20 HistoStar Operator Guide A81010100 Issue 2 Cold Module Cold Module - Rear View 23 - Cold Plate 28 - Mains Power Connector 24 - Timer Control Switch 29 - Rating Plate 25 - Cold Plate Timer Connector 30 - Serial Number Label...

  • Page 21: System Interfacing

    Chapter 1 - Introducing HistoStar System Interfacing Using the Touchscreen The HistoStar has an embedded touchscreen user interface which is used to set all preferences and timer functions. Note Avoid using sharp or pointed objects to press buttons on the touchscreen.
  • Page 22 HistoStar Operator Guide A81010100 Issue 2 Buttons The following image buttons occur throughout in the user interface. Button Image Description Action(s) Return Returns the user to the previous screen Left and Right Used to scroll through lists Used to increase or decrease values and...

  • Page 23: System Specification

    Chapter 1 - Introducing HistoStar System Specification Embedding Module Physical Specification Height (doors closed) 430 mm 17 in Height (doors open) 670 mm 26½ in Width 650 mm 25½ in Depth 587 mm 23 in Weight (without wax) 25 kg...
  • Page 24 HistoStar Operator Guide A81010100 Issue 2 Cold Module Physical Specification Height 365 mm 14½ in Width 430 mm 17in Depth 585 mm 23 in Weight 20kg 44lbs Electrical Specification Voltage 220V~ - 240V~ 100 - 120V~ Frequency 50 / 60 Hz...

  • Page 25: Chapter 2 - Basic Operation

    Chapter 2 - Basic Operation Safety Before operating the HistoStar , ensure that the following safety statements have been read and understood. Do not use the HistoStar in the presence of combustible chemicals as fire or explosion may occur. Molten wax is hot and can cause severe burns.

  • Page 26: Switching On Histostar

    5 minutes in order to allow the refrigeration system to stabilise. Note This procedure assumes that the HistoStar is set up to run with the Cold Module attached and set for 'Timer' control. HistoStar can melt 2kg of Histoplast PE wax pellets from solid in approximately 7 hours, so sufficient time between initial switching on and required time of use should be allowed.
  • Page 27 To exit Sleep Mode touch the 'ON' button at the bottom of the screen. Note If HistoStar has been in Sleep Mode for an extended period of time, it may be necessary to wait a while whilst the Cold Module achieves its operating temperature.

  • Page 28: Operating Method

    • Turn on HistoStar and allow the temperatures of both modules to stabilise - see Switching On HistoStar. • Place the processed tissue cassettes in the tissue storage tank.
  • Page 29 Chapter 2 - Basic Operation • Use forceps to remove the specimen from the cassette and position it as required within the base mould. Notes Take care when handling specimens to ensure they are not dropped onto the work areas. Dropping specimens may result in cross-contamination of tissue.
  • Page 30 HistoStar Operator Guide A81010100 Issue 2 • Move the base mould back onto the hot spot and place the cassette onto the base mould. • Fill the cassette with wax. Note Use the wax flow control knob to adjust the wax flow rate to a suitable setting.
  • Page 31 Chapter 2 - Basic Operation • Transfer the base mould and cassette onto the cold plate for rapid chilling. Once fully cooled and set, the cassette can be removed from the base mould ready for sectioning. • Notes A frost build-up on the cold plate is normal. The rate of frost build-up will vary with the humidity in the laboratory.

  • Page 32: Using The Para Trimmer

    HistoStar Operator Guide A81010100 Issue 2 Using the Para Trimmer The Para Trimmer surface on the Embedding Module can be used to remove excess wax from an embedded cassette. To clean wax from an embedded cassette: • Ensure the hot spot is at its preset operating temperature - the Para Trimmer temperature is determined by the hot spot settings.

  • Page 33: Switching Off Histostar

    Switching Off HistoStar Enter Sleep Mode To switch HistoStar into its power saving Sleep Mode touch the 'Sleep' button at the bottom of the Main screen. Note If the Cold Module is set to timer control, entering Sleep Mode will turn it off. The cold spot and hot spots on the embedding module will also be switched off.

  • Page 35: Chapter 3 - Setup

    If the Cold Module has been purchased as a stand-alone unit, the outer packaging will differ from that shown; however, the sections which refer to the specific unpacking of the Cold Module will still apply. Unpacking the Modules To unpack the HistoStar modules: • Remove the lid and the outer sleeve and shown.
  • Page 36 HistoStar Operator Guide A81010100 Issue 2 • Lift off the accessories box and set aside for unpacking later. • Lift out the cold module using the handles in the cardboard packing. The cold module weighs approximately 20kg, with uneven weight distribution - seek help when...
  • Page 37 Chapter 3 - Setup Ensure that the unit is carried in the manner shown below - failure to do so may result in personal injury and / or damage to the unit. • Fold down the side panels of the packing, and manoeuvre the cold module out of the foam pieces as shown.
  • Page 38 HistoStar Operator Guide A81010100 Issue 2 • Lift out the embedding module using the handles in the cardboard packing. The embedding module weighs approximately 25kg - seek help when lifting if necessary. Ensure that the unit is carried in the manner shown below - failure to do so may result in...
  • Page 39 Chapter 3 - Setup • Fold down the side panels of the packing, and manoeuvre the embedding module out of the foam pieces as shown.
  • Page 40 HistoStar Operator Guide A81010100 Issue 2 Assembling HistoStar To assemble the various components: • Place the Tissue Storage Tray, Forceps Block Cover and Waste Tray in the locations shown. Place the Tissue Storage Cover over the Tissue Storage Tank as shown.
  • Page 41 Chapter 3 - Setup • Tilt the cover backwards and slide the raised edge at the back underneath the bottom edge of the Base Mould Storage Compartment. This allows the raised edge to act as a drawer-stop, preventing the cover from being accidentally pulled too far forward.

  • Page 42: Connections

    HistoStar Operator Guide A81010100 Issue 2 Connections Connection to Mains Supply To connect HistoStar to the Mains Supply: Ensure that each module is positioned within easy reach of a suitably rated Mains Power Supply - see • System Specification. •...
  • Page 43 Chapter 3 - Setup Ensure the timer control lead is inserted in the correct orientation as shown. • Connect the loose end of the timer control lead into the cold plate timer connector on the Embedding Module. • Switch the timer control switch to the 'TIMER' position.
  • Page 44 HistoStar Operator Guide A81010100 Issue 2 Footswitch Connection To connect the optional footswitch: • Insert the open end of the footswitch tubing into the footswitch connector port on the Embedding Module. Ensure the tubing is fully inserted otherwise the footswitch may not operate correctly.

  • Page 45: Setup And Location

    The air vents below the cold spot on the Embedding Module should be unobstructed at all times. • • It is advisable to ensure that there is also at least 75mm (3in) of clearance at the sides of the HistoStar .
  • Page 46 Do not position the HistoStar directly under air-conditioning or heating vents, or near to an open window. • Do not position the HistoStar in an area where it is likely that the operator could be bumped or knocked during embedding.
  • Page 47 The correct levelling should have the rear of the instrument approximately 1mm higher than the front. • Once the instrument is levelled correctly, tighten the lock-nuts to secure the adjustable feet. Note The levelling of the HistoStar does not need to be particularly precise and the factory setting should be suitable for most cases.

  • Page 48: Setting Workflow Direction

    HistoStar Operator Guide A81010100 Issue 2 Setting Workflow Direction HistoStar is designed to allow the user to determine how it is arranged, in order to suit their workflow and laboratory layout. The number of modules can also be varied as required.
  • Page 49 Chapter 3 - Setup The most common alternative arrangement is shown below: It is also possible to create unique workflows by varying the number of each type of module - see below for example. Note Only one Cold Module can be timer controlled from a single Embedding Module.

  • Page 50: Filling With Wax

    HistoStar Operator Guide A81010100 Issue 2 Filling with Wax To fill the wax tank: • Open the wax tank cover to allow access to the wax tank. Use a beaker or other suitable container to pour pre-molten wax into the wax tank.
  • Page 51 Chapter 3 - Setup Note It is possible to fill the wax tank with wax pellets instead of pre-molten wax, however, the pellets will take a significant amount of time to melt. It is recommended that wax pellets are only added directly to the wax tank when there is still about a quarter of a tank of molten wax still in the tank.

  • Page 52: Dispense Lever Adjustment And Wax Flow Control

    HistoStar Operator Guide A81010100 Issue 2 Dispense Lever Adjustment and Wax Flow Control When dispensing wax it may be desirable to reposition the dispense lever and / or adjust the flow rate of the wax to suit your specific working habits.
  • Page 53 Chapter 3 - Setup • Reposition the dispense lever mechanism as required.. When repositioning the dispense lever always ensure that the wax dispense flow control knob is set to the lowest possible setting, to avoid accidentally dispensing hot wax whilst moving the mechanism. Tighten the dispense lever position adjustment knob to lock the dispense lever mechanism in the new •...
  • Page 54 HistoStar Operator Guide A81010100 Issue 2 Adjusting the Wax Dispense Flow rate To adjust the wax dispense flow rate turn the wax dispense flow control knob to the required setting. Notes Turning the adjustment knob counter-clockwise will increase the flow of wax; turning the adjustment knob clockwise will reduce the flow of wax.

  • Page 55: System Settings

    Chapter 3 - Setup System Settings There are various settings on the HistoStar which can be adjusted as necessary. These settings are: Time and Date • • Temperature Units • Temperature Set Points • Timer Settings Display and Audio •...
  • Page 56 HistoStar Operator Guide A81010100 Issue 2 • Press the 'Menu' button to display the Main Menu screen.

  • Page 57: Time And Date

    Chapter 3 - Setup Time and Date To set the time and date: Press the 'Timer' button on the Main Menu. • • Press the 'Set Time / Date' button on the Time Menu.
  • Page 58 Press the 'Return' button to exit the screen when finished. Note HistoStar does not start to count the time until the 'Set Time / Date' screen has been exited. HistoStar does not automatically adjust the time to allow for Daylight Saving Time.

  • Page 59: Temperature Units

    Chapter 3 - Setup Temperature Units To change the units used for temperature readings between degrees Celsius (°C) and degrees Fahrenheit (°F): • Press the 'Options' button in the Main Menu. • Press the 'Units' button in the Options Menu - the value shown will change to the alternate unit type. •...

  • Page 60: Temperature Set Points

    HistoStar Operator Guide A81010100 Issue 2 Temperature Set Points The HistoStar allows individual control of each of the hot and cold areas. To define the temperature set points: • Press the 'Status' button in the Main Menu. • When the Status Menu appears, select the item to control.
  • Page 61 Chapter 3 - Setup Timer Button The 'Timer' button allows the timer settings for the day to be activated / deactivated as required. Note The 'Timer' button is an On / Off switch only and does not link to the Program Timer screen. To edit the timer settings see Timer Settings.
  • Page 62 HistoStar Operator Guide A81010100 Issue 2 Note All of the heated areas have the same maximum and minimum values. To exit the Settings screen: • Press the 'Return' button. • The settings will be saved and the Status Menu will appear.

  • Page 63: Timer Settings

    Timer Settings Introduction The timer function allows the user to define specific times when the HistoStar will be used. This allows the software to pre-heat / pre-cool the relevant sections of the instrument, so that they are ready for use at a given time.
  • Page 64 HistoStar Operator Guide A81010100 Issue 2 • Press the 'Program Timer' button in the Time Menu. • The Program Timer' screen will appear. Disable Timer To disable the timer function for any given day: • In the Program Timer screen, use the 'Left' and 'Right' buttons to scroll through the days of the week, until the required day is displayed.
  • Page 65 Chapter 3 - Setup Edit Timer Values To edit the 'Ready Time' and / or 'Off Time' for any given day: • In the Program Timer screen, use the 'Left' and 'Right' buttons to scroll through the days of the week, until the required day is displayed.

  • Page 66: Display And Audio

    HistoStar Operator Guide A81010100 Issue 2 Display and Audio To change the display and audio settings: • Press the 'Options' button in the Main Menu. • Press the 'Display' button in the Options Menu.
  • Page 67 Chapter 3 - Setup • The Display Settings screen will appear. Adjust Contrast The screen contrast can be adjusted as follows: • In the Display Settings screen press the required contrast value from '1' to '5'. • '5' is the darkest setting and '1' is the lightest. •...

  • Page 68: Instrument Lighting

    Instrument Lighting The work areas on HistoStar are illuminated by two sets of lights for improved user visibility during embedding. One set of lights is located at the end of the wax dispense arm and are referred to as 'Hot Spot Lights'.
  • Page 69 Chapter 3 - Setup • The Light Menu screen will appear. Adjust Hot Spot Lights Settings To adjust the hot spot lights settings: • Press the 'Hot Spot Light' button to access the Hot Spot Lights Settings screen. • Use the 'Up' and 'Down' buttons to increase or decrease the 'Idle Minutes' setting.
  • Page 70 HistoStar Operator Guide A81010100 Issue 2 • Use the 'Left' and 'Right' buttons to scroll through the activation options below: This setting links the hot spot lights to the hot spots, so that they are both on / off at the same time.
  • Page 71 Chapter 3 - Setup Adjust Accessory Lights Settings To adjust the accessory lights settings: • Press the 'Accessory Light' button to access the Accessory Lights Settings screen. • Use the 'Left' and 'Right' buttons to scroll through the activation options below: This setting links the accessory lights to the hot spot lights so that they are both on / off at the same time.
  • Page 72 HistoStar Operator Guide A81010100 Issue 2 Accessory Lights Power Settings To adjust the accessory lights power settings: • In the Accessory Lights Settings screen, press the 'Power Options' button to access the Accessory Lights Power Settings screen. • Select a power setting between '1' and '5'; where '5' is the brightest, and '1' is the most dim.

  • Page 73: Forceps Block Power Settings

    Chapter 3 - Setup Forceps Block Power Settings To adjust the forceps block power setting: Press the 'Options' button in the Main Menu. • • Press the 'Forceps' button in the Options Menu.
  • Page 74 HistoStar Operator Guide A81010100 Issue 2 • The Forceps Options screen will appear. • Press the 'Forceps Block' button to display the Forceps Block Settings screen. • Select a power setting between '1' and '5'; where '5' is the warmest, and '1' is the coolest.

  • Page 75: Language Selection

    Chapter 3 - Setup Language Selection To change the language which the user interface is displayed in: Press the 'Options' button in the Main Menu. • Press the 'Language' button in the Options Menu. •...
  • Page 76 HistoStar Operator Guide A81010100 Issue 2 • The Language screen will appear. • Use the 'Up' and 'Down' buttons to scroll through the available languages. Press the 'Return' button to return to the previous screen. • Note The Language screen can also be accessed from the Startup screen by pressing the 'Language' button in the bottom...

  • Page 77: Touchscreen Calibration

    Switch HistoStar 'Off' using the power switch - see Switching Off HistoStar. • Wait until the touchscreen goes blank and then place a finger on the touchscreen. Keeping the finger firmly in contact with the touchscreen, switch HistoStar 'On' using the power switch - see • Switching On HistoStar.
  • Page 78 HistoStar Operator Guide A81010100 Issue 2 • Touch the 'A' button - the screen will change to the following: • Touch the 'B' button to complete the calibration sequence. • HistoStar will now begin to initialise as normal.

  • Page 79: Electric (Heated) Forceps

    - see Appendix A - Spares and Accessories. The heated forceps come in 3 different tip sizes and have been designed specifically for use with the HistoStar. Only use HistoStar forceps with the HistoStar - use of other forceps may cause damage to the instrument and / or the forceps.
  • Page 80 HistoStar Operator Guide A81010100 Issue 2 Connecting the Electric Forceps The electric forceps are connected via the socket at the front of the embedding module. There is also a small hook which can be used to guide the wire to the left-hand side of the dispense if required.
  • Page 81 Chapter 3 - Setup Once connected, the electric forceps should be located in the smaller, front holes of the forceps block when not in use.
  • Page 82 HistoStar Operator Guide A81010100 Issue 2 Using the Electric Forceps The electric forceps must be switched on before they can be used. To switch the electric forceps on: • Press the 'Options' button in the Main Menu. • Press the 'Forceps' button in the Options Menu.
  • Page 83 Chapter 3 - Setup • Press the 'Electric Forceps' button when the Forceps Options screen appears. • Press the 'Electric Forceps' button to toggle the electric forceps 'On' and 'Off'. Ensure the electric forceps are at the correct temperature before use. The tips of the electric forceps are hot during use - only hold the forceps using the insulated grips.
  • Page 84 HistoStar Operator Guide A81010100 Issue 2 Cleaning the Electric Forceps To clean the electric forceps: • With the electric forceps switched 'On' and at their working temperature, use a dry lint-free cloth to wipe any excess wax off the tips.

  • Page 85: Chapter 4 - Troubleshooting

    Chapter 4 - Troubleshooting Troubleshooting Tables Touch Screen Display Problem Possible Cause Remedy Display will not turn Unit is not powered Switch the embedding module off for 30 seconds and then back on again to reset the control system Check that the Mains Lead is connected correctly - see Connections Key touch position is Touchscreen positional...

  • Page 86: Wax Tank And Dispense

    HistoStar Operator Guide A81010100 Issue 2 Wax Tank and Dispense Problem Possible Cause Remedy Wax will not dispense Insufficient level of wax Add wax to wax tank in wax tank Wax has not yet melted Allow time for wax to melt...
  • Page 87 Chapter 4 - Troubleshooting Wax tank will not heat Wax tank heater not Switch on the wax tank heater - see Temperature Set Points switched on Instrument Switch the embedding module off for 30 seconds and then back on malfunction again to reset the control system Compare the set point temperature to the actual temperature - if the set point temperature is not achieved after a suitable length of...

  • Page 88: Forceps Block

    HistoStar Operator Guide A81010100 Issue 2 Forceps Block Problem Possible Cause Remedy Does not heat up Power level too low Increase the power level of the forcep block - see Temperature sufficiently to melt wax off for wax in use...

  • Page 89: Hot Spot

    Chapter 4 - Troubleshooting Hot Spot Problem Possible Cause Remedy Hot spot will not heat up Hot spot heater not Switch on the hot spot heater - see Temperature Set Points switched on Instrument malfunction Switch the embedding module off for 30 seconds and then back on again to reset the control system Compare the set point temperature to the actual temperature - if the set point temperature is not achieved after a suitable...

  • Page 90: Tissue Storage Tank

    HistoStar Operator Guide A81010100 Issue 2 Tissue Storage Tank Problem Possible Cause Remedy Wax not fully melted Wax has not yet Allow time for wax to melt melted Ensure temperature setting is high enough to melt the wax being used...

  • Page 91: Temperature Sensors

    Chapter 4 - Troubleshooting Temperature Sensors Problem Possible Cause Remedy Display shows current Sensor malfunction Perform Heater System Check temperature as 'XXX' Call Thermo Fisher Scientific Service Department...

  • Page 92: Base Mould Storage

    HistoStar Operator Guide A81010100 Issue 2 Base Mould Storage Problem Possible Cause Remedy Base mould storage does Base mould storage Ensure heater is switched on - see Temperature Set Points not heat up heater is not switched Ensure temperature setting is correct...

  • Page 93: Cold Spot

    Chapter 4 - Troubleshooting Cold Spot Problem Possible Cause Remedy Inadequate cooling Surface of cold spot has Allow sufficient time for the cold spot to cool. not reached the required temperature yet Fan air flow blocked Remove blockage from fan inlet directly underneath cold spot Instrument malfunction Switch the embedding module off for 30 seconds and then back on again to reset the control system Call Thermo Fisher Scientific Service Department...

  • Page 94: Cold Plate

    HistoStar Operator Guide A81010100 Issue 2 Cold Plate Problem Possible Cause Remedy Inadequate cooling Ambient temperature Reduce the ambient temperature or increase air circulation is above 27°C around instrument Condenser air-flow is Remove blockage from fan inlets blocked Instrument Call Thermo Fisher Scientific Service Department...

  • Page 95: Electric Forceps

    Chapter 4 - Troubleshooting Electric Forceps Problem Possible Cause Remedy Do not heat up or fail to Power is not switched Ensure electric forceps are switched on - see Electric Forceps reach working Ensure temperature setting is correct temperature Faulty or poor Ensure the jack is fully inserted into the connection socket connection Failure of heating...

  • Page 96: Poor Sectioning

    HistoStar Operator Guide A81010100 Issue 2 Poor Sectioning Problem Possible Cause Remedy Poor Sectioning Wax tank too hot Check the melting point of the wax and reduce the temperature setting as required - see Temperature Set Points. Contaminated or Drain the wax tank - see Draining the Wax Tank •...

  • Page 97: Heater System Check

    Chapter 4 - Troubleshooting Heater System Check To assist the service team in troubleshooting your problem, please carry out the following system checks and record the values before calling the Thermo Fisher Scientific Service Department for technical support: From the 'Status Menu' press the button for the required heater. •...

  • Page 99: Chapter 5 - Cleaning And Maintenance

    Chapter 5 - Cleaning and Maintenance Safety Before carrying out any cleaning or maintenance on the HistoStar , ensure that the following safety statements have been read and understood. Check HistoStar for damage prior to performing any cleaning or maintenance. If any damage is noted, remove the unit from service immediately and contact the Thermo Fisher Scientific Service Department.

  • Page 100: General Instrument Cleaning

    To ensure that this instrument remains in peak condition, it is strongly recommended that it is serviced annually by a Thermo Fisher Scientific approved Service Engineer. When cleaning the surfaces of HistoStar , ensure any necessary precautions are taken to avoid cross-contamination of specimens.

  • Page 101: Daily Tasks

    Chapter 5 - Cleaning and Maintenance Daily Tasks The following cleaning tasks should be carried out after use: • Wipe down the main surfaces to remove molten wax. Empty the waste wax tray. • Note In the case of a large spillage on the main deck, pay close attention to the level of wax in the waste wax tray and empty as required.

  • Page 102: Weekly Cleaning

    HistoStar Operator Guide A81010100 Issue 2 Weekly Cleaning The following cleaning tasks should be carried out about once every week: • Remove the forceps block cover and wipe away any wax. Clean the touchscreen. • Remove waste wax tray and clean around the drain holes and the metal plate that the tray rests on.

  • Page 103: Fuse Replacement

    Fuse Replacement To replace the fuses: • Ensure HistoStar is switched off and disconnected from the mains power supply. • Remove the power lead from the mains inlet connector. Open the hinged cover to allow access to the fuse drawer.
  • Page 104 HistoStar Operator Guide A81010100 Issue 2 • Remove the fuse drawer from the recess. Note It may be necessary to use a suitable lever to remove the fuse drawer. • Remove the fuses from the fuse drawer and replace. Note Always replace fuses as a pair.

  • Page 105: Emptying The Waste Wax Drawer

    Chapter 5 - Cleaning and Maintenance Emptying the Waste Wax Drawer To empty the waste wax drawer: Note It is advisable to ensure that the tissue storage tank is switched on when emptying as this will ensure that the wax remains molten and does not solidify in the bottom of the removable waste tray.
  • Page 106 HistoStar Operator Guide A81010100 Issue 2 • Dispose of the waste wax according to local regulations. • Use a dry paper towel to wipe any molten wax off the waste tray and the metal plate that the drawer rests on in the instrument.

  • Page 107: Using The Wax Draining Assembly

    Chapter 5 - Cleaning and Maintenance Using the Wax Draining Assembly The Wax Draining Assembly is used to aid safe and efficient draining of molten wax from the HistoStar. The Wax Draining Assembly is made up of several components which must be assembled before use.
  • Page 108 When fitting the wax draining funnel, ensure that the slides on the edges of the funnel are located on the drawer runners as shown below. The HistoStar should be positioned so that the funnel hole overhangs the edge of the worktop, allowing the assembly to hang below it.
  • Page 109 • Place a suitable, leak-proof container on the base. Notes A 2.5kg Thermo Scientific Histoplast box (including the internal plastic bag) is ideal. Maximum recommended dimensions of the container are 170 x 165 x 210mm (W x D x H).
  • Page 110 HistoStar Operator Guide A81010100 Issue 2 • The Wax Draining Assembly is now ready to use. See also: Draining the Tissue Storage Tank Draining the Wax Tank...

  • Page 111: Draining The Tissue Storage Tank

    Chapter 5 - Cleaning and Maintenance Draining the Tissue Storage Tank To drain the tissue storage tank: • Ensure the wax in the tissue storage tank is molten and the tissue storage tank is switched on. Note It is advisable to ensure that the hot-spot is switched on when draining as this will ensure that the wax remains molten until it has exited the internal pipework.
  • Page 112 HistoStar Operator Guide A81010100 Issue 2 • To drain the wax, pull the drain valve lever outwards as shown. • The wax should drain out of the tissue storage tank and into the wax draining assembly.
  • Page 113 Chapter 5 - Cleaning and Maintenance The box containing the drained wax may be heavy when draining is complete - take care when lifting. If the tissue storage tank has been overfilled, it may be necessary to change the box part way through the draining process.

  • Page 114: Draining The Wax Tank

    HistoStar Operator Guide A81010100 Issue 2 Draining the Wax Tank To drain the wax tank: • Ensure the wax in the wax tank is molten. Notes It is advisable to ensure that the hot-spot and the tissue storage tank are switched on when draining as this will ensure that the wax remains molten until it has exited the internal pipework.
  • Page 115 Chapter 5 - Cleaning and Maintenance • Fit the wax draining assembly - see Using the Wax Draining Assembly. To drain the wax, push the dispense lever towards the back of the instrument as shown. •...
  • Page 116 HistoStar Operator Guide A81010100 Issue 2 • The wax will drain out of the wax tank and into the wax draining channels and out of the drain hole. • The wax should drain from the drain hole and into the wax draining assembly.
  • Page 117 Chapter 5 - Cleaning and Maintenance Note After the wax has stopped being dispensed, allow at least 10 minutes for the residual wax in the drainage channels to drain into the wax draining assembly. When removing the wax draining assembly, immediately insert the waste wax tray to catch any further residue. Take care to avoid spilling molten wax during draining.

  • Page 119: Appendices

    Appendices Appendix A - Spares and Accessories Accessories Part Description Number Tamping tools kit - set of 2 - 1 x 20mm and 1 x A81010030 12mm A81010031 Spatula A81010026 Cassette lid storage cover A81010032 Magnifier assembly AP16629 Footswitch A81010033 Electric, heated forceps - Red - 1mm tip A81010034 Electric, heated forceps - Yellow - 2mm tip...
  • Page 120 HistoStar Operator Guide A81010100 Issue 2 Waxes (Paraffins) Part Number Description Qty. 6774006 Histoplast 1 x 2.5kg 6774060 Histoplast 1 x 10kg 8330 Histoplast PE 8 x 1kg 8331 Histoplast IM 8 x 1kg 8332 Histoplast LP 8 x 1kg...

  • Page 121: Appendix B - Packing Instructions, Removal From Use And Disposal

    Appendices Appendix B - Packing Instructions, Removal from Use and Disposal If the HistoStar has been used with, or has come into contact with hazardous material, ensure that the appropriate decontamination procedures have been followed (See World Health Organization 'Laboratory Biosafety Manual').

  • Page 122: Appendix C - Screen Maps

    HistoStar Operator Guide A81010100 Issue 2 Appendix C - Screen maps...

  • Page 123: Appendix D - Approved Reagent List

    Appendices Appendix D - Approved Reagent List The following reagents have been approved for use with HistoStar. Note Use of unapproved reagents may cause damage to the instrument and / or samples. Waxes (Paraffins) Histoplast Histoplast LP Histoplast PE Histoplast IM...

  • Page 125: Index

    Index Dispense Flow Control .......... 9 Accessories ............1, 111 Dispense Lever ......... 9, 20, 106 Adjustable Feet ............. 37 Dispense Lever Position ........44 Air-Vents .............. 37 Display Settings ..........47, 58 Altitude ..............15 Drain Hole ............106 Audio Settings ..........
  • Page 126 HistoStar Operator Guide A81010100 Issue 2 Levelling ............... 37 Lights .............. 60 Temperature Set Points ........52 Lights ..............60 Location ............... 37 Temperature Units .......... 51 Time ............... 49 Main Menu ............ 47, 55 Timer .............. 55 Mains Power Connector ......9, 34, 95 Touchscreen Calibration .........
  • Page 127 Index Waste Wax Tray ........9, 103, 106 WEEE Directive ............ 1 Wax Dispense Flow Control ......... 44 Weekly Tasks ............94 Wax Draining Container ......103, 106 Weight ..............15 Wax Tank ............42 Width ..............15 Cover ..............8 Workflow ............

Table of Contents

Save PDF