Thermo Scientific HM340E Operation Manual
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Thermo Scientific HM340E Operation Manual

Rotary microtome
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Thermo Scientific
HM340E
Rotary Microtome
Product Code 905190
Operation Manual – English
ex Ser. No 44481
387863 Issue 8

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Summary of Contents for Thermo Scientific HM340E

  • Page 1 Thermo Scientific HM340E Rotary Microtome Product Code 905190 Operation Manual – English ex Ser. No 44481 387863 Issue 8...

  • Page 3: Company Information

    The development of Thermo Scientific products and services is an ongoing process. Please ensure that any published information you use as a reference is up to date and relates to the condition of the product. If necessary, check with your local Thermo Fisher Scientific representative.

  • Page 4: Symbols

    Symbols The following symbols and conventions may be used throughout this document and on the instrument: This symbol is used on the instrument, or in a document, to indicate that instructions must be followed for safe and correct operation. If this symbol appears on the instrument, always refer to the operator guide.

  • Page 5: Table Of Contents

    Thermo Scientific Rotary Microtome HM 340E Table of Contents COMPANY INFORMATION ............................3 SYMBOLS ..................................4 TABLE OF CONTENTS ..............................5 CHAPTER 1 - SAFETY INFORMATION ........................... 7 ................................. 7 ENERAL AFETY ................................8 HEMICAL AFETY ................................... 8 NVIRONMENT ............................... 8...
  • Page 6 Thermo Scientific Rotary Microtome HM 340E ..............................50 ECTIONING NSTRUCTIONS Conditions of Knife/BladeEdge ..........................50 Setting the Cutting angle ............................50 How to Avoid Errors ..............................52 Possible Sources of Errors – Cause and Removal ..................... 53 CHAPTER 5 – MAINTENANCE AND CARE ........................54 .................................

  • Page 7: Chapter 1 - Safety Information

    Chapter 1 - Safety Information Thermo Scientific instruments are designed for convenient and reliable service; however, improper use or handling by a user may damage the instrument, or cause a hazard to health. The instrument must not be used in a manner not specified by Thermo Shandon Limited.

  • Page 8: Chemical Safety

    Thermo Scientific Rotary Microtome HM 340E Chemical Safety The introduction of chemicals creates potential hazards. Thermo Fisher Scientific has adopted the following position with regard to the subject of volatile chemicals used in laboratories: • Customers using non-specified chemicals in the instrument, do so at their own risk.

  • Page 9: Emc Statement

    Thermo Scientific Rotary Microtome HM 340E EMC Statement This IVD equipment complies with the emissions and immunity requirements of IEC 61326-2-6:2006. This equipment has been designed and tested to CISPR 11 Class A. It is intended for use in a laboratory environment, by a trained and qualified professional. In a domestic environment it may cause radio interference, in which case it may be necessary to take measures to mitigate the interference.

  • Page 10: Chapter 2 - Introduction

    The HM340E will cut sections in a range from 0,5 µm up to 100 µm. For the protection of knife and specimen, the instrument retracts the specimen at the end of the cut. If desired, the function <retraction> can be turned off.

  • Page 11: Scope Of Delivery Standard Equipment

    Thermo Scientific Rotary Microtome HM 340E Scope of Delivery Standard Equipment The Rotary Microtome HM340E is supplied with the following accessories: Quantity Description Operating Panel Section waste tray, big Cover plate, brushed aluminium Cover Brush Paraffin repellent Para Gard, 100 ml...

  • Page 12: Additional Equipment

    Thermo Scientific Rotary Microtome HM 340E Additional Equipment Additional available equipment (optional) Description Cat. No. Section Transfer System STS with Blade Holder TE 771200 Standard Specimen Clamp for STS 715550 Transfer Surface for STS 715550 with Universal Cassette Clamp 771110...
  • Page 13 Thermo Scientific Rotary Microtome HM 340E Segment Arc 715590 Universal Specimen Holder 715060 Large Field Magnifier, 220 V 760160 Large Field Magnifier, 120 V 760170 Zoom-Stereomicroscope Zeiss Stemi 2000 755210 Adapter for Stemi 2000 532090 Ring Illumination, 100….240V/50…60Hz 760340 SEC 35...

  • Page 14: Technical Data Sheet

    Thermo Scientific Rotary Microtome HM 340E Technical Data Sheet Microtome HM340E Resolution 0,5 µm from 0,5 – 5 µm 1 µm from 5 – 20 µm 2 µm from 20 – 30 µm 5 µm from 30 – 60 µm 10 µm from 60 –...
  • Page 15 Thermo Scientific Rotary Microtome HM 340E Microtome HM340E Operating Conditions for indoor use only + 10° C up to + 40° C (at a max. rel. humidity of 60 %) altitude up to 2000 M.S.L. Floor Loading Requirements 110 kg/m2 Power Requirements 100...240 V 1,6 A +/-10%...

  • Page 16: Chapter 3 - Operating Instructions

    Thermo Scientific Rotary Microtome HM 340E Chapter 3 – Operating Instructions Setting up the Microtome Cut through the bands around the carton. • Open the carton. • Remove the accessories. • On the lower front and rear side of the instrument, there are two recessed grip to lift and carry the microtome.

  • Page 17: Frontal View

    Thermo Scientific Rotary Microtome HM 340E Frontal View Rear View...

  • Page 18: Lateral View Left Side

    Thermo Scientific Rotary Microtome HM 340E Lateral View Left Side Lateral View Right Side...

  • Page 19: Initial Start-Up

    Thermo Scientific Rotary Microtome HM 340E Initial Start-up Note The type of examination materials used and all special conditions for their processing, pre-treatment and, if necessary, storage as well as instrument controls for correct and safe operation are the responsibility of the operator.

  • Page 20: Operating Panel

    Thermo Scientific Rotary Microtome HM 340E Operating Panel The operating elements of the panel are clearly arranged and allow for a safe operation of the instrument. Note The operating panel can be removed from the instrument and be used free standing.

  • Page 21: Display And Key Function

    Thermo Scientific Rotary Microtome HM 340E Display and Key Function Thermo Scientific Microtomes are designed to support your workflow.To achieve best results, take your time to get know the HM340E thoroughly before starting work. Hand Wheel Brake For your personal safety, the hand wheel brake must always be activated when working on the specimen holder or knife/blade carrier.

  • Page 22: Cutting Process Indication

    Thermo Scientific Rotary Microtome HM 340E Cutting Process Indication In the middle line of the display information about the sectioning status can be seen. • Press the “scroll button“ [2], to show a list of the functions on the display.
  • Page 23 Thermo Scientific Rotary Microtome HM 340E This value shows the sum of the sections already cut in microns. Trim- ming values and sectioning values are added up. To reset to zero press “section counter” reset button [3]. Remaining Travel to Front End Position This value shows the distance in microns, which is left for sectioning in microns.

  • Page 24: Setting Section Thickness And Trimming Thickness

    Thermo Scientific Rotary Microtome HM 340E Setting Section Thickness and Trimming Thickness The required section and trimming thicknesses are set with the knob [A]. • To choose between section thickness and trimming thickness, press the knob [A] In the NORMAL display mode the thickness range is indicated in the display.
  • Page 25 Thermo Scientific Rotary Microtome HM 340E The graduation of the trimming thicknesses is divided into four ranges: Range Graduation up to 30 µm 5 µm from 30 µm to 100 µm 10 µm from 100 µm to 200 µm 20 µm from 200 µm to 500 µm...

  • Page 26: Cutting Movement And Retraction

    Thermo Scientific Rotary Microtome HM 340E Fine Feed After having adjusted knife and specimen and having trimmed the specimen, sectioning can be started. • Press the knob [A] to select the FEED section thickness setting. In the NORMAL display mode, an outline around the value is shown on the display.

  • Page 27: Specimen Course Feed

    Thermo Scientific Rotary Microtome HM 340E Specimen Course Feed After changing the specimen or moving the knife or knife carrier, it is necessary to adjust the specimen to the knife edge again. This can easily be done by means of the specimen coarse feed and the defined trimming values.
  • Page 28 Thermo Scientific Rotary Microtome HM 340E • Select the display mode by turning the knob [A] and confirm it by pressing it. The normal display mode shows the selected fine and trim section thickness with additional status indications at the same time.
  • Page 29 Thermo Scientific Rotary Microtome HM 340E Turning off the Retraction Function If desired, the function “Retraction“ can be turned off. • Press the MENU button [1]. • Select submenu “Retraction“ by turning the knob [A]. • Press the knob to confirm the selection.
  • Page 30 Thermo Scientific Rotary Microtome HM 340E Language Selection for the Display The information on the display can be shown in different languages. • Press the MENU button [1]. • In the sub-menu select „Languages“ by turning the knob [A] •...

  • Page 31: Factory Defaults

    Thermo Scientific Rotary Microtome HM 340E Factory Defaults With this option, you return all settings to factory defaults. HM340E Factory Defaults Retraction Language German Fine 0,5 µm Trim 5 µm Note By selecting this function, language is reset to german.

  • Page 32: Rocking Mode Function

    Thermo Scientific Rotary Microtome HM 340E Rocking Mode Function Newly installed, allows you to perform manual sectioning in rocking mode. To cut the sample, you just move the hand wheel up and down. Setting the rocking Function • Press the ROCK button [4] to start Rocking function.

  • Page 33: Adapters For Specimen Clamping

    Thermo Scientific Rotary Microtome HM 340E Adapters for Specimen Clamping When adjusting the specimen the mechanical hand wheel brake must be locked in and the knife guard has to be used to cover the knife/blade edge. Adapter, Non-Orienting This adapter serves for a non-orienting fastening of the specimen clamps directly onto the cylinder of the instrument.

  • Page 34: Changing And/Or Fastening Specimen Clamps

    Thermo Scientific Rotary Microtome HM 340E Changing and/or Fastening Specimen Clamps The available specimen clamps are all fastened or removed in the same way. • To change the specimen clamping system, press the clamping lever [a] downwards and pull it to the side.

  • Page 35: Re-Adjusting Specimen Clamps

    Thermo Scientific Rotary Microtome HM 340E Re-Adjusting Specimen Clamps Note Frequent use of the clamping lever can result in loss of optimal clamping strength. If the necessary readjustment is not carried out, it might be possible that the specimen clamp does not clamp anymore.

  • Page 36: Specimen Clamping

    Thermo Scientific Rotary Microtome HM 340E Specimen Clamping Note To clamp specimens, different systems are available. With the orienting adapter it is simple to align the specimen properly to the knife. Universal Cassette Clamp The universal cassette clamp allows a quick change system.

  • Page 37: Standard Specimen Clamp

    Thermo Scientific Rotary Microtome HM 340E The unrestricted use of the Macro-UCC is only possible with ER blade carrier. Note Maximum 68mm x 50mm specimen size can be cut. The Paraffin blocks in MacrOflow-Cassettes are 70-72mm long and should be trimmed to 68mm length manually.

  • Page 38: Insert For Round Specimens, V-Insert And V-Distance Piece

    Thermo Scientific Rotary Microtome HM 340E Insert for Round Specimens, V-Insert and V-Distance Piece To cut round specimens, the insert for round specimens with defined diameters of 6, 15, 19 and 25 mm (special sizes on request) or the V- insert can be clamped into the standard specimen clamp.
  • Page 39 Thermo Scientific Rotary Microtome HM 340E Foil Clamp The foil clamp is used for foils or thin specimens. • To insert the specimen, loosen the three clamping screws [a] slightly and push the movable jaw [c] sideways against the two springs [b].
  • Page 40 Thermo Scientific Rotary Microtome HM 340E Segment arc and universal Specimen holder The segment arc and universal specimen holder are highly suitable for the clamping of small specimens embedded in plastic. • The specimen is inserted in the holder [a] and clamped with the screw [b] with the hex head wrench [c].

  • Page 41: Knife And Blade Carriers

    Thermo Scientific Rotary Microtome HM 340E Knife and Blade Carriers Due to moving parts on the knife/blade holder and the extrem sharp- ness of the microtome knife or blade, a danger area arises, which might lead to hand injuries if the safety features and the instruction manual are not followed carefully.
  • Page 42 Thermo Scientific Rotary Microtome HM 340E Using High Profile Blades When using high profile blades, first remove the spacer strip [i]. • For this, turn the clamping lever [g] to the front until it stops. • Pull off the clamping lever [g] and remove the clamping plate [e].

  • Page 43: Disposable Blade Carrier E

    Thermo Scientific Rotary Microtome HM 340E Disposable Blade Carrier E The disposable blade carrier E is designed to take all commercially available high and low profile blades. Inserting the Blade Insert the blade into the slot behind the clamping plate [e] •...
  • Page 44 Thermo Scientific Rotary Microtome HM 340E Clearance Angle Adjustment The clearance angle between cutting edge and specimen can be shifted and adjusted to the requirements of the tissue to be sectioned. • Loosen the clamping lever [h] on the right side of the blade carrier and move the upper part [k] of the blade carrier on the base [a].

  • Page 45: Knife Carrier C

    Thermo Scientific Rotary Microtome HM 340E Knife Carrier C Inserting the Knife • To insert the knife, the clamping screws [g] must be unscrewed slightly so the knife can be pushed in from the side. • The height of the knife is adjusted with the two knurled nuts [n].
  • Page 46 Thermo Scientific Rotary Microtome HM 340E Moving the Knife Carrier on the Console Loosen the clamping lever [f ] on the left of the knife carrier to move the carrier forwards and backwards on the guide bars. This allows a rough adjustment of knife and specimen.

  • Page 47: Readjusting Knife Or Blade Carriers

    Thermo Scientific Rotary Microtome HM 340E Readjusting Knife or Blade Carriers Frequent use of the clamping levers can cause the knife or blade carriers not to optimally clamp any longer. If the necessary readjustments are not made, it might even be possible that the knife or blade carriers can- not be clamped anymore.

  • Page 48: Section Waste Tray With Integrated Arm Rest

    Thermo Scientific Rotary Microtome HM 340E Section Waste Tray with Integrated Arm Rest The section waste tray fully surrounds the knife/blade holder and has an integrated arm rest, permitting fatigue- free use of the microtome. It is easy to clean and can be removed at any time by being pulled towards the user.

  • Page 49: Installing The Large Field Magnifier On The Microtone

    Thermo Scientific Rotary Microtome HM 340E Installing the Large Field Magnifier on the Microtone Please use the enclosed fastening elements to attach the magnifier to the microtome. • Remove the cover plate on the upper side of the microtome hood.

  • Page 50: Chapter 4 - Working With The Microtome

    Thermo Scientific Rotary Microtome HM 340E Chapter 4 – Working with the Microtome Due to moving parts on the knife/blade holder and the extrem sharp- ness of the microtome knife or blade, working with the microtome might lead to hand injuries if the safety instructions given in this manual are not carefully attended to.
  • Page 51 Thermo Scientific Rotary Microtome HM 340E An angle, which is too steep, is also unfavorable as the knife/blade might vibrate too much while sectioning. The so-called “chatters“ appear on the block surface as well as on the sections as parallel stripes having negative effects on the microscopical evaluation.

  • Page 52: How To Avoid Errors

    Thermo Scientific Rotary Microtome HM 340E How to Avoid Errors Specimen preparation: When preparing specimens, be sure that a suitable embedding medium, fixation, dehydration and infiltration time are chosen. Specimen temperature: Sectioning is carried out at ambient temperature (excluding frozen sections). If the temperature is too high, the paraffin softens.

  • Page 53: Possible Sources Of Errors - Cause And Removal

    Thermo Scientific Rotary Microtome HM 340E Possible Sources of Errors – Cause and Removal Problem Cause Solution blunt knife/blade move knife/blade or insert a new one knife/blade angle unfavorable adjust knife/blade angle, until an optimal clearance angle angle can be found...

  • Page 54: Chapter 5 - Maintenance And Care

    Thermo Scientific Rotary Microtome HM 340E Chapter 5 – Maintenance and Care Cleaning and Care Cleaning and care of the microtome should be carried out daily. Please proceed as follows: • Turn off the power switch of the instrument. •...

  • Page 55: Maintenance

    Thermo Scientific Rotary Microtome HM 340E Maintenance Before starting sectioning, instrument, knife or blade carrier and section waste tray should be treated with commercially available paraffin repellent. Note This considerably reduces the adhesive force of paraffin waste on the indi- vidual parts of the microtome.

  • Page 56: Replacement Work

    Thermo Scientific Rotary Microtome HM 340E Replacement Work Replacing the Fuses The two power fuses are located above the power switch [a] on the rear side of the microtome. • To replace the two fuses, turn off the power switch [a] of the instru- ment and unplug it.

  • Page 57: Chapter 6 - Conditions For Transportation

    Thermo Scientific Rotary Microtome HM 340E Chapter 6 – Conditions for Transportation Returning the Instrument for Repair or Routine Maintenance Repair or maintenance works is normally carried out at the site of installation. If this is not possible for some specific reasons, the instrument can be returned to Thermo Fisher Scientific.

  • Page 58: Measure For Taking Out Of Operation

    Thermo Scientific Rotary Microtome HM 340E Measure for taking out of Operation • Turn off the power switch of the instrument. • Activate the mechanical hand wheel brake. • Remove knife or blade and store it in a safe place.

  • Page 59: Disposal Of The Instrument After Final Shutdown

    Thermo Scientific Rotary Microtome HM 340E Disposal of the Instrument after Final Shutdown After the final shutdown of the instrument, we recommend to contact a local recycling company for the disposal according to the nationally applicable regulations. Under no circumstances is it allowed to dispose of the instrument together with ordinary domestic waste.

  • Page 60: Index

    Thermo Scientific Rotary Microtome HM 340E Index Installing the Large Field Magnifier on the Microtone ..49 Intended use ................10 Introduction .................10 Accessories ................59 Adapter, Non-Orienting ............33 Adapter, Orienting, Specimen Orientation ......33 Adapters for Specimen Clamping ........33 Knife and Balde Carriers ............41...
  • Page 61 Thermo Scientific Rotary Microtome HM 340E Specimen Clamping ............. 36 Specimen Course Feed ............27 Standard Specimen Clamp ........... 37 Universal Cassette Clamp .............36 Symbols ................. 4 Universal Cassette Clamp, Adjustable ........36 Technical Data Sheet ............14 Warranty Statement ..............8...

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905190

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