Thermo Scientific Microm HM355S Operation Manual page 62

Working with the Microtome
Sectioning Instructions
e knife/blade must never be placed on an angle that is too at since
the knife/blade does not cut into the block at all this way. Only the rear
side slides over the sectioning surface.
An angle, which is too steep, is also unfavorable as the knife/blade
might vibrate too much while sectioning. e so-called "chatters"
appear on the block surface as well as on the sections as parallel stripes
having negative e ects on the microscopical evaluation.
Operation Manual HM335S
Thermo Fisher Scientific 61
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