How To Avoid Errors - Thermo Scientific Microm HM355S Operation Manual

Working with the Microtome

How to Avoid Errors

How to Avoid Errors
Specimen preparation:
When preparing specimens, be sure that a suitable embedding medium,
xation, dehydration and in ltration time are chosen.
Specimen temperature:
Sectioning is carried out at ambient temperature (excluding frozen
sections). If the temperature is too high, the para n softens. erefore,
avoid heating para n specimens by direct exposure to sunlight or other
near sources of heat.
Tightening the clamping screws:
Tighten all clamping screws and clamping levers on the knife/blade
carrier, specimen holder and specimen orientation.
Selection of the knife/blade:
Carefully select the required knife/blade material and pro le.
Adjustment of the knife/blade:
Carefully adjust the proper clearance angle of the knife: 5 – 7° for glass
knives. For settings for diamond knives ask the knife manufacturer.
Select a clearance angle adjustment of 5 – 15° according to the facet
angle. Typically, adjust an angle of 10 – 12°. Take care to adjust knife
height.
Cutting speed:
Always cut at proper speed.
Note!
General rule: e harder the material, the slower the cutting speed!
Trimming:
Carefully bringing the knife/blade and specimen together.
Operation Manual HM335S
Thermo Fisher Scientific 63
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