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Summary of Contents for Thermo Scientific Heraeus Megafuge 8
- Page 1 Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge Instruction Manual 50143328-c • 10 / 2016...
- Page 3 Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge Instruction Manual 50143328-c • 10 / 2016...
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Page 6: Weee Compliance
WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:... -
Page 7: Table Of Contents
Heraeus Megafuge 8 ........ - Page 8 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 Heraeus Megafuge 8 / 8R Centrifuge...
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Page 9: Preface
The hematocrit value is determined using the instructions written in the instruction manual of the hematocrit rotor. The instructions are based upon the reference method following DIN 58933, allowing to determinate the erythrocyte volume fraction within the blood. This centrifuge has to be operated by trained specialists only. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 10: Precautions
The centrifuge should be operated by trained specialists only. In order to ensure safe operation of the Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge, general safety regulations must be followed. Mind the regulations in your country. Set Up Conditions WA RN I N G Plug the centrifuge only into sockets which have been properly grounded. -
Page 11: Hazardous Substances
In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service. Maintenance WA RN I N G The centrifuge housing is not to be opened by the operator. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 12: Symbols Used On The Centrifuge
WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. This symbol refers to electrical hazards. Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 13: Technical Specifications
Height (open door / closed door) 660 mm / 310 mm Width 370 mm Depth 490 mm Weight 35 kg Front Side Measurement, 1 m in front of the instrument at 1.6 m height Without Rotor Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 14: Heraeus Megafuge 8R
Height (open door / closed door) 700 mm / 320 mm Width 460 mm Depth 670 mm Weight 74 kg Front Side Measurement, 1 m in front of the instrument at 1.6 m height Without Rotor Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 15: Directives, Standards And Guidelines
IEC 61010-2-101, 2nd Edition EN ISO 13485 China IEC 61010-1, 2nd Edition 230 V, 50 / 60 Hz IEC 61010-2-020, 2nd Edition IEC 61010-2-101, 2nd Edition EN 61326-1 Class B EN 61326-2-6 EN ISO 13485 Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 16: Mains Supply
Rotor Selection The Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge is supplied without a rotor. Various rotors are available. If not stated otherwise the rotors can be used in the Thermo Scientific Heraeus Megafuge 8 centrifuge as also in the Thermo Scientific Heraeus Megafuge 8R centrifuge. -
Page 17: Transport And Set Up
WA R N IN G Always lift the centrifuge from both sides. Never lift the centrifuge by its front panel, its back panel or at its door. Always remove the rotor before moving the centrifuge. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 18: Leveling
Hoirzontal level is to be checked when moving the centrifuge to a new location. Mains Connection NO T I CE Plug the centrifuge into grounded electrical sockets only. Turn off the power supply switch. Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 19: Storage
Before shipping the centrifuge please bear the following in mind: ƒ The centrifuge must be clean and decontaminated. The decontamination must be confirmed in a decontamination certificate. Contact customer service for more ƒ details. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 21: Control Panel
Control Panel The control panel contains the keys and displays of the centrifuge (only the power switch is located on the right side (Heraeus Megafuge 8R) or on the backside (Heraeus Megafuge 8) of the device). Heraeus Megafuge 8 No . -
Page 22: Heraeus Megafuge 8R
TOGGLE Key for Speed / RCF Value Use the TOGGLE key to change the display mode. (Speed / RCF Value). Program Keys Use the Program Keys to save and load programs. “” on page 25 Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 23: Operation
WAR N ING Do not reach into the gap between the centrifuge door and the housing. Use the emergency release only for malfunctions and power failures (see “Mechanical Emergency Door Release” on page 39). Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 24: Rotor Installation
The centrifuge door should audibly click into place. Rotor Installation The approved rotors for the Thermo Scientific Heraeus Megafuge 8 / 8R are listed in section “Rotor Selection” on page 14. Use only the rotors from this list in the centrifuge. Permitted accessories are listed in the rotor manuals. -
Page 25: Acceleration / Deceleration Rates
Close the centrifuge door. Acceleration / Deceleration Rates The Thermo Scientific Heraeus Megafuge 8 / 8R offers you 2 profiles: standard and soft. The setting is displayed above the ACCELERATION / DECELERATION key. Press the ACCELERATION / DECELERATION key to cycle through and set the available profiles. The LEDs show the chosen settings. -
Page 26: Pre-Selecting Speed/Rcf
Press the START key to accept or wait 4 secondes until the centrifuge automatically saves the chosen values. Moving to setting speed / RCF or temperature also automatically stores the set value. Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 27: Continuous Operation
When the set temperature is reached, the centrifuge will beep and continue to hold the temperature. Press the STOP key to end the prewarming or precooling. The display shows the current temperature inside the rotor chamber. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 28: Programs
Programs The Thermo Scientific Heraeus Megafuge 8 Centrifuge is able to save up to 4 programs; the Thermo Scientific Heraeus Megafuge 8R is able to save up to 99 programs. It is only possible to save a program if the centrifuge is in standstill. -
Page 29: Starting The Centrifuge Run
Once the run is complete, the rotor and load should be checked, ensuring that all buckets are greased and can swing free and that the tubes are balanced by following the rotor instruction manual. For information on troubleshooting, see “Troubleshooting by Guide” on page 40 Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 30: Stopping The Centrifuge Run
Short-term Centrifugation For quick centrifugation, the Thermo Scientific Heraeus Megafuge 8 / 8R has a PULSE-function. By holding down the PULSE key, spinning will start at maximum acceleration rate, continue at maximum rotor speed until the key is released. The rotor then decelerates at maximum rate. Preset values for acceleration, deceleration and speed or RCF are disregarded for the PULSE function. -
Page 31: Aerosol-Tight Rotors
C AU TI ON Skin can be pierced by sharp mandrel tip. Do not touch the mandrel. Switch off Centrifuge To turn off the centrifuge push the mains switch to “0”. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 33: System Menu
A total number of cycles completed on the centrifuge is counted, since installation or a new main board was installed. Cycle counts should be periodically recorded to help with the identification of the total number of runs a rotor has completed. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... - Page 34 Chapter 5 | Flowchart System Menu Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific...
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Page 35: Maintenance And Care
Any signs of corrosions, such as rust or white / metalic pitting, the rotor or accessories should be immediately removed from service. Particular attention should be taken with the bottom of buckets on swing out rotors Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 36: Cleaning
Drive and door lock can be damaged by entering liquids. Do not allow liquids, especially organic solvents, to get on the drive shaft, the drive bearings or the centrifuge door locks. Organic solvents break down the grease in the motor bearing. The drive shaft could lock up. Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 37: Cleaning The Filter Mat
Be sure the disinfectant can drain off the rotor. Rinse the rotor and accessories thoroughly with water and then rub down. Place the rotors on a plastic grate with their cavities pointing down, to allow to fully drain and dry. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 38: Decontamination
ƒ The maximum permissible autoclave cycle is 20 minutes at 121 °C. ƒ Clean the rotor before autoclaving and rinse it with distilled water. Remove all accessories (tubes, adapters) from the rotor. Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 39: Service Of Thermo Fisher Scientific
For the countries of the European Union the disposal is regulated by the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. (“WEEE Compliance” on page 4) Mind the information on transport and shipping. (“Transporting” on page 15 and „Shipping the Centrifuge“ on page Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 41: Troubleshooting
Pull the release cord attached to it to trigger the mechanical door release. The centrifuge door will open and the samples can be removed. Open the centrifuge door. Mechanical Emergency Door Release located on the right side Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... -
Page 42: Troubleshooting By Guide
Restart the centrifuge. E-005; If the error message appears again, inform the service. E-008; E-010; E-011; E-012; E-015; E-016; E-034; E-036; E-041; E-048; E-050; E-051; E-052; E-053; E-054; E-072; E-077; E-101; E-104 Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... - Page 43 E-017; Read Manual Wait until the rotor has stopped. E-020; Check, if the rotor is qualified for the Heraeus Megafuge 8 / 8R Centrifuge E-021; (check “Rotor Selection” on page 20). E-022; Check, if the bottom of the rotor is...
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Page 44: When To Contact Customer Service
For a description how to get there, see „Enter system menu“ on page 36. You will find a description on how to find the „Software ID“ on page 37 and for the „NVRAM ID“ on page Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific... -
Page 45: Chemical Compatibility Chart
Hydroxide (10%) Ammonium Hydroxide (28%) Ammonium Hydroxide (conc.) Ammonium Phosphate Ammonium Sulfate Amyl Alcohol Aniline Sodium Hydroxide (<1%) Sodium Hydroxide (10%) Barium Salts Benzene Benzyl Alcohol Boric Acid Cesium Acetate Cesium Bromide Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge... - Page 46 Ethyl Acetate Ethyl Alcohol (50%) Ethyl Alcohol (95%) Ethylene Dichloride Ethylene Glycol Ethylene Oxide Vapor Ficoll-Hypaque™ Hydrofluoric Acid (10%) Hydrofluoric Acid (50%) Hydrochloric Acid (conc.) Formaldehyde (40%) Glutaraldehyde Glycerol Guanidine Hydrochloride Haemo-Sol™ Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific...
- Page 47 Solution 555 (20%) Magnesium Chloride Mercaptoacetic Acid Methyl Alcohol Methylene Chloride Methyl Ethyl Ketone Metrizamide™ Lactic Acid (100%) Lactic Acid (20%) N/Butyl Alcohol N/Butyl Phthalate N-Dimethyl- formamide Sodium Borate Sodium Bromide Sodium Carbonate (2%) Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge...
- Page 48 Sucrose Sucrose, Alkaline Sulfosalicylic Acid Nitric Acid (10%) Nitric Acid (50%) Nitric Acid (95%) Hydrochloric Acid (10%) Hydrochloric Acid (50%) Sulfuric Acid (10%) Sulfuric Acid (50%) Sulfuric Acid (conc.) Stearic Acid Tetrahydrofuran Heraeus Megafuge 8 / 8R Centrifuge Thermo Scientific...
- Page 49 Chemical resistance data is included only as a guide to product use. Because no organized chemical compatibility data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge...
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Page 51: Index
Switch on Centrifuge Symbols Entering Parameters Technical Data Technical Specifications Imbalance Temperature Adaptation during Standstill Intended Use Thermo Scientific Heraeus Megafuge 8R 11, 12, Items Supplied Transport and Set Up Transporting the Centrifuge Troubleshooting Location WEEE Mains Supply Maintenance and Care... - Page 52 Thermo Scientific Heraeus Megafuge 8 Thermo Electron LED GmbH Country of Origin: Thermo Fisher (Suzhou) Instruments Co., Ltd. Zweigniederlassung Osterode Am Kalkberg No. 297 Taishan Road, New District, Suzhou, Jiangsu 37520 Osterode am Harz P. R. China Germany Thermo Scientific Heraeus Megafuge 8R...
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