Related Manuals for Thermo Scientific Acclaim HILIC-10
Summary of Contents for Thermo Scientific Acclaim HILIC-10
- Page 1 Thermo Scientific Acclaim HILIC-10 Columns Product Manual P/N: 065339-02 January 2013 Part of Thermo Fisher Scientific...
- Page 2 Product Manual Acclaim HILIC-10 Analytical Column Acclaim HILIC-10, 3 µm, Analytical, 4.6 x 150 mm (P/N 074257) Acclaim HILIC-10, 3 µm, Analytical, 3.0 x 150 mm (P/N 074258) Acclaim HILIC-10, 3 µm, Analytical, 2.1 x 150 mm (P/N 074259) Acclaim HILIC-10, 5 µm, Analytical, 4.6 x 150 mm (P/N 079693) Acclaim HILIC-10, 5 µm, Analytical, 4.6 x 150 mm (P/N 079694)
- Page 3 Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Revision History: Revision 02, January, 2013, Rebranded for Thermo Scientific. Added 5 µm and 1.9 µm column formats. For Research Use Only. Not for use in diagnostic procedures. Thermo Scientific...
- Page 4 Highlights information necessary to prevent damage to software, loss of data, or invalid test IMPORTANT results; or might contain information that is critical for optimal performance of the system. Highlights helpful information that can make a task easier. Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 4 of 23 065339-02...
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Page 5: Table Of Contents
Column washing procedure ......................... 12 5. Frequently Asked Questions ................14 What is the difference between the Acclaim HILIC-10 column and other HILIC columns? ...... 14 Why do I need an Acclaim HILIC-10 column? ................... 14 When do I need an Acclaim HILIC-10 column? ..................14 What factors should I consider for method development using this column? .......... - Page 6 Sodium Chloride ............................21 6.12 Tris•HCl ............................... 21 6.13 α-Tocopherol and α-Tocopherol Acetate ..................... 22 6.14 Vitamins K1 and K2 ............................ 22 6.15 Retinol Palmitate and Retinol Acetate ......................23 Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 6 of 23 065339-02...
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Page 7: Introduction
– Introduction 1. Introduction The Thermo Scientific™ Acclaim™ HILIC-10 is a general-purpose silica based HILIC (Hydrophilic Interaction Liquid Chromatography) column for separating highly hydrophilic analytes. It is based on high-purity, spherical, porous silica gel that is covalently modified with a proprietary hydrophilic layer. -
Page 8: Physical Data
6000 psi 0.2 – 0.4 mL/min 2.1 x 150 mm 10,000 psi 0.2 – 0.4 mL/min 1.9 µm 2.1 x 250 mm 15,000 psi 0.2 – 0.4 mL/min Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 8 of 23 065339-02... -
Page 9: Step-By-Step User Guide
Degas the aqueous component of the mobile phase and then add the solvent component. Avoid excessive purging or degassing of mobile phases containing solvents, if possible, since the volatile solvent can be ‘boiled’ off from the solution. NOTE Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 9 of 23 065339-02... -
Page 10: Step 3 - Set Up The Lc System
NOTE Step 6 – Real sample analysis Once you are satisfied with the column performance report result, the column is ready for your application. Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 10 of 23 065339-02... -
Page 11: Considerations In Method Development
For a 4.6 x 150-mm column, the typical injection volume is 5 to 10 µL. However, an injection volume as low as 1 or 2 µL is not uncommon. Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 11 of 23 065339-02... -
Page 12: Column Care
3. Before any injection is made, the column should be equilibrated with the mobile phase thoroughly. For a normal phase method that uses aqueous or acetonitrile immiscible organic solvents: Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 12 of 23 065339-02... - Page 13 For a 3.0 mm i.d. column, the typical flow rate for washing is 0.3 mL/min. NOTE If above treatment fails to revive the column, the column should be replaced. Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 13 of 23 065339-02...
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Page 14: Frequently Asked Questions
Unlike other HILIC columns that have covalently bonded neutral hydrophilic surfaces, such as amide, diol, or cyano functionality, the Acclaim HILIC-10 column is based on a modified high- purity, spherical, porous silica with a proprietary hydrophilic ligand. As a result, the Acclaim HILIC-10 exhibits different polarity and selectivity compared to other HILIC columns. -
Page 15: What Should I Do Before Starting To Use The Acclaim Hilic-10 Column
– Frequently Asked Questions 5.6 What should I do before starting to use the Acclaim HILIC-10 column? Read this Product Manual carefully, and contact Thermo Fisher Scientific Technical Support if you have any questions regarding the use of this column. -
Page 16: Applications
CN/10 mM (total) NH OAc, pH5 Temperature: 30 °C Flow Rate: 1 mL/min Inj. Volume: 1 µL Detection: UV at 220 nm Peaks: mg/mL 1. Salicylic acid 2. Aspirin Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 16 of 23 065339-02... -
Page 17: Melamine And Cyanuric Acid
OAc, pH5 Temperature: 30 °C Flow Rate: 1 mL/min Inj. Volume: 2 µL Detection: ELSD Sample: Melamine (0.2 mg/mL in mobile phase) 85% CH 80% CH Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 17 of 23 065339-02... -
Page 18: Carbohydrates
OAc, pH5 Temperature: 60 °C Flow Rate: 1 mL/min Inj. Volume: 5 µL Detection: ELSD Peaks: (0.2 mg/mL in mobile phase) 1. Xylitol 2. Sorbitol 3. Inositol Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 18 of 23 065339-02... -
Page 19: Acrylamide And Acrylic Acid
Temperature: 30 °C Flow Rate: 1 mL/min Inj. Volume: 2 µL Detection: UV at 230 nm Peaks: (0.1 mg/mL each) 1. Methacrylic acid 2. Acrylic acid Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 19 of 23 065339-02... -
Page 20: Acrylic Acid And Oligomers
Flow Rate: 1 mL/min Inj. Volume: 10 µL Detection: ELSD Peaks: (0.1 mg/mL each in mobile phase) 1. TAPS 2. CHES 3. MOPS 4. TES 5. HEPES Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 20 of 23 065339-02... -
Page 21: Sodium Chloride
OAc, pH5 Temperature: 30 °C Flow Rate: 1 mL/min Inj. Volume: 10 µL Detection: ELSD Peaks: Tris•HCl (0.2 mg/mL in mobile phase) 1. Cl 2. Tris•H Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 21 of 23 065339-02... -
Page 22: Α-Tocopherol And Α-Tocopherol Acetate
Flow Rate: 1 mL/min Temperature: 30 °C Inj. Volume: 5 µL Detection: UVD-340U; UV 265 nm; spectra 200-400 nm Peaks: µg/mL 1. Vitamin K1 2. Vitamin K2 Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 22 of 23 065339-02... -
Page 23: Retinol Palmitate And Retinol Acetate
94.75/5.00/0.25 v/v/v Heptane:Tetrahydrofuran:Isopropanol Flow Rate: 1 mL/min Temperature: 30 °C Inj. Volume: 5 µL Detection: UVD-340U; UV 325 nm Peaks: µg/mL 1. Retinol Palmitate 2. Retinol Acetate Minutes Thermo Scientific Product Manual for Acclaim HILIC-10 Columns Page 23 of 23 065339-02...
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