Thermo Scientific HESI-II Probe User Manual
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Thermo Scientific HESI-II Probe User Manual

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HESI-II Probe
User Guide
70005-97000 Revision C
April 2009

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Summary of Contents for Thermo Scientific HESI-II Probe

  • Page 1 HESI-II Probe User Guide 70005-97000 Revision C April 2009...
  • Page 2 © 2009 Thermo Fisher Scientific Inc. All rights reserved. Ion Max is a trademark of Thermo Fisher Scientific Inc. in the United States. Xcalibur is a registered trademark of Thermo Fisher Scientific Inc. in the United States. PEEK is a trademark of Victrex PLC. The following are registered trademarks in the United States and other countries: KEL-F is a registered trademark of 3M Corporation.
  • Page 3 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 4 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...

  • Page 5: Table Of Contents

    Chapter 3 Removing and Installing the HESI-II Probe ....... .9 Removing the HESI-II Probe .

  • Page 7: Preface

    Preface This HESI-II Probe User Guide provides you with information on using the heated-electrospray ionization probe. It also provides procedures for installing and maintaining the HESI-II probe. Safety and Special Notices Make sure you follow the precautionary statements presented in this guide. The safety and other special notices appear in boxes.

  • Page 8: Precautions For Handling The Hesi-Ii Probe

    When operating the mass spectrometer in the heated-electrospray mode, do not touch the heated surfaces of the HESI-II probe or the Ion Max API source housing. Touching the heated surfaces of the probe or the source housing can cause burns when the probe is operating at typical temperatures of 350 to 450 °C.

  • Page 9: Contacting Us

    To suggest changes to documentation or to Help • Complete a brief survey about this document by clicking the link below. Thank you in advance for your help. • Send an e-mail message to the Technical Publications Editor at techpubs-lcms@thermofisher.com. Thermo Scientific HESI-II Probe User Guide...

  • Page 11: Introduction

    ; and ammonium ions (NH ), yielding an m/z value of [M + 18] This chapter describes the principles of the heated-electrospray ionization technique through use of the HESI-II probe (see Figure 1) and a Thermo Scientific mass spectrometer. Figure 1.
  • Page 12 5. Electrostatic repulsion ejects sample ions from the very small, highly charged droplets into the gas phase. 6. The sample ions enter the mass spectrometer through the ion transfer tube. Figure 2 shows the steps in the formation of ions from highly charged droplets. HESI-II Probe User Guide Thermo Scientific...
  • Page 13 You can use the HESI-II probe in either positive or negative ion polarity mode. The ion polarity mode of choice is determined by the polarity of the preformed ions in solution: Acidic molecules form negative ions in solution, and basic molecules form positive ions.
  • Page 14 Tip For best results, avoid operating the HESI-II probe at elevated temperatures without solvent flow from the LC system or the syringe pump. Allowing the HESI-II probe to run dry at elevated temperatures can cause blockage of the replaceable metal needle (see “Replacing the Needle Insert”...

  • Page 15: Chapter 2 Functional Description

    1 μL/min to 1 mL/min without splitting. The removable components of the HESI-II probe are the end cover that is secured to the probe body with socket head screws and the needle insert that screws into the probe body...
  • Page 16 Grounding union holder Sample and solvent enter and exit the HESI-II probe through the needle insert, which protrudes from the sample inlet port at the back of the probe and from the nozzle at the front of the probe (see Figure 4).
  • Page 17 600 °C. For recommended operating temperatures and gas flow settings, see Table 1 page Figure 5. Sheath gas plumbing Sheath gas fitting (blue) in gas inlet (S) Sheath ESI needle (outer needle) Metal needle (inner needle) Thermo Scientific HESI-II Probe User Guide...
  • Page 18 Metal needle (inner needle) The angle of the HESI-II probe is fixed at approximately 60 degrees. To help optimize the spray stability, use adjustment screws on the Ion Max source housing to make small changes to probe position. The fixed angle, off-axis spraying affords long-term signal stability (robustness) for most solutions that contain non-volatile matrix components, mobile phase buffers, or ion-pairing reagents.

  • Page 19: Removing And Installing The Hesi-Ii Probe

    Removing and Installing the HESI-II Probe This chapter describes how to set up the API source with the HESI-II probe. You must remove the HESI-II probe to perform maintenance on the probe. Note To install the APCI probe, refer to the Ion Max and Ion Max-S API Source Hardware Manual.
  • Page 20 Connection between grounding union and sample inlet Probe locking knob 3. Disconnect the 8 kV cable from the HESI-II probe high voltage receptacle as follows (see Figure 8 Figure a. Unlock the cable by twisting the locking ring counterclockwise. b. Unplug the 8 kV cable from the HESI-II probe high voltage socket.
  • Page 21 Removing and Installing the HESI-II Probe Removing the HESI-II Probe 4. Unplug the vaporizer cable from the HESI-II probe vaporizer cable socket (see Figure 9 Figure 10). Figure 9. Top view of the API source housing and the HESI-II probe...
  • Page 22 Turn the probe counterclockwise until the guide pin is free of the interlock block. c. When the guide pin is free of the interlock block, pull the probe out of the port. 10. Store the HESI-II probe in its original shipping container. HESI-II Probe User Guide...

  • Page 23: Installing The Hesi-Ii Probe

    Note To remove an APCI or ESI probe, refer to the Ion Max and Ion Max-S API Source Hardware Manual. To install the HESI-II probe 1. Remove the HESI-II probe from its storage container. Inspect and clean it if necessary. Figure 12 shows the locations of the probe guide pin, vaporizer cable connector socket, sheath and auxiliary gas inlets, 8 kV cable connector socket, sample inlet, and grounding union holder.
  • Page 24 Removing and Installing the HESI-II Probe Installing the HESI-II Probe 2. Turn the probe locking knob counterclockwise until the probe locking ring is opened to its widest position (see Figure 13). Figure 13. Ion Max API source housing without a probe...
  • Page 25 6. Unplug the vaporizer cable connector from the interlock socket and connect it to the vaporizer cable connector socket on the HESI-II probe. To align the connector pins with the socket, align the red dot on the connector with the red dot on the socket.
  • Page 26 Figure 16 shows the HESI-II probe installed in the Ion Max API source. Figure 16. HESI-II probe installed in the Ion Max API source housing Vaporizer cable Auxiliary gas line (green fitting)

  • Page 27: Adjusting The Probe Position

    Removing and Installing the HESI-II Probe Adjusting the Probe Position Adjusting the Probe Position To maximize sensitivity, you can adjust the probe depth. If you have the Ion Max API source housing, you can also adjust the side-to-side and front-to-back probe position by a few millimeters.
  • Page 28 Removing and Installing the HESI-II Probe Adjusting the Probe Position Figure 17. Top view of the Ion Max source housing with a HESI-II probe Side-to-side Knurled nut Front-to-back CAUTION - HOT SURFACE Side-to-side position set to 0 Micrometer set to 1.75...

  • Page 29: Maintenance

    Replacing the needle insert requires a partial disassembly of the probe. Tip For best results, flush the HESI-II probe at the end of each working day, using a 50:50 LCMS-grade methanol/distilled water solution from the LC pump through the HESI-II probe.

  • Page 30: Replacing The Needle Insert

    Figure 18. Exploded view of the needle insert Metal needle ESI needle Needle guide fitting and ferrule Adjustable union Figure 19. Metal needle insert assembly with an enlarged view of the stainless steel needle tip ESI needle Metal 0.5 mm needle HESI-II Probe User Guide Thermo Scientific...
  • Page 31 Replacing the Needle Insert To support flow rates from 1 to 2000 μL/min, Thermo Fisher Scientific provides two needle inserts for the HESI-II probe. The difference between the two inserts is the size of the metal needle and supporting ferrule (Table Table 3.
  • Page 32 7. Insert the two M4×35 mm length, socket head cap screws into the end cover, and then tighten them with a 3 mm (7/64 in.) hex wrench or ball driver. 8. Reinstall the HESI-II probe (see “Installing the HESI-II Probe”...

  • Page 33: Chapter 5 Replaceable Parts

    HESI-II Probe Kit ........

  • Page 35: Index

    HESI-II probe photograph removable components of the HESI-II probe red alignment markers sheath gas plumbing replaceable parts front-to-back probe position safety precautions for handling the HESI-II probe viii heated auxiliary gas, description sheath gas plumbing diagram side-to-side probe position Thermo Scientific...
  • Page 36 Index: V voltages, ESI needle WEEE compliance HESI-II Probe User Guide Thermo Scientific...

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