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When using a buffer, obtain a high-quality reagent and filter it through a 0.45 micron filter
such as the type in the Eluent Clarification Kit available from Waters.
Using Clean Water
When operating a system with aqueous eluents, use high-purity, filtered water such as that
provided by reverse-osmosis, ion-exchange water systems.
Using Clean Filters
The 600E system contains an eluent filter. To avoid drawing foreign particles into the
pump where they can accumulate and affect pump operation, the eluent reservoir filter
should be kept clean.
Cleaning Filters
If a stainless steel filter becomes plugged, clean it by sonicating in 6 N nitric acid (HNO
for 5 to 10 minutes.
B.3 Eluent Miscibility
Changing Between Eluents
Before changing from one eluent to another, see
the eluents to be used. The following considerations apply when changing eluents:
•
Make a change directly from one eluent to another if the two eluents are miscible.
•
When changing between two eluents that are not totally miscible (for example, from
hexane to water), flush the system with an intermediate eluent (such as methanol).
See
Section 3.6.2, Changing Between Incompatible
Considering Temperature
You must consider the effect of temperature on eluent solubility if operating at an elevated
or reduced temperature. Temperature does affect eluent miscibility.
Using Buffers
Aqueous buffers may precipitate when mixed with organic eluents. Therefore, take the
following precautions:
•
When switching from a high-ionic-strength buffer to an organic eluent, flush the
buffer out of the system with distilled water before adding the organic eluent.
212
Eluent Considerations
Table B-1
to determine the miscibility of
Eluents.
)
3
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