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Prepared your system as outlined in
Operation
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Set operating parameters as described in
Operating Parameters
9.1 Setting Run Conditions
Before you make a run with the PowerLine controller in the Isocratic mode, you must first
set the run conditions. This section describes the procedures for:
•
Setting pump parameters
•
Setting external event switches
Run Requirements
To perform an isocratic run in the isocratic mode, you must:
•
Set the run conditions (flow rate, eluent composition, sparge rate, heater module
temperature, and external switch output settings)
•
Set the run time
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Equilibrate the system
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Start the run
166
Running Gradient Controller in Isocratic Mode
Chapter 3, Preparing Your 600E System for
Chapter 8, Setting Gradient Controller
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