Documenting The Gradient Table - Waters 600e User Manual

This section describes:
•

Documenting the gradient table

• Programming the gradient table
Note: When creating gradient, event, and detector tables, be sure that you design the
time-related parameters specified in each table to work effectively with the time-related
parameters in the other two tables of the same set.
6.1.1 Documenting the Gradient Table
Define the information you want in a gradient table before you actually program a gradient
table. This will minimize the need for you to reprogram the table later.
Using the Analysis Plan and File Sheet
Appendix C, Analysis
defining and documenting gradient-table information.
1. Make a photocopy of the Analysis Plan and File Sheet.
2. On each line of the Analysis Plan and File Sheet, write:
• Gradient segment time (time is cumulative from injection)
• Flow rate
• Eluent composition
• Gradient profile curve number
The number of changes needed to execute the gradient determines the total number
of lines. The gradient table can contain up to 15 lines (14 plus initial conditions).
Note: You must create a gradient table to include isocratic pump parameters in a
method. A gradient table with isocratic pump parameters consists of a single line.
See Section 6.1.2, Programming the Gradient
3. When the table entries are complete, keep the Analysis Plan and File Sheet as a
permanent record of your table.
Returning to Initial Conditions
When making multiple injections that require flow or compositional gradients, include a
line in the gradient table to return the system to initial conditions or to reequilibrate the
system before the next injection.
Plan, contains an Analysis Plan and File Sheet to assist you in
Table.
Creating a Gradient Table 103