Solvent Miscibility; Organic Solvents For Use With The 1525 Hplc Pump - Waters 1525M Installation And Maintenance Manual

Table C-4 Organic Solvents for Use with the 1525 HPLC Pump
4-Cyanophenol
Acetone
Acetonitrile
Amyl acetate
Benzaldehyde
Benzene
Benzyl alcohol
Butanol
Carbon
tetrachloride
C.3 Solvent Miscibility
Before changing solvents, refer to
be used. When changing solvents, be aware that:
• Changes involving two miscible solvents may be made directly. Changes involving
two solvents that are not totally miscible (for example, from chloroform to water),
require an intermediate solvent (such as methanol).
• Temperature affects solvent miscibility. When running a high-temperature application,
consider the effect of the higher temperature on solvent solubility.
• Buffers dissolved in water may precipitate when mixed with organic solvents.
When switching from a strong buffer to an organic solvent, flush the buffer out of the
system with distilled water before you add the organic solvent.
Organic Solvents
Chloroform Ethylene glycol
Cyclohexane Formaldehyde
Cyclohexanone Heptane
Dibutyl phathalate Hexane
Dimethyl formamide Isooctane
Dimethyl sulfoxide Isopropanol
Ethanol Lysine hydrochloride Xylene
Ethyl acetate Methanol
Ethylene dichloride Methyl ethyl ketone
Table C-5 to determine the miscibility of the solvents to
Methylene chloride
n-Propanol
Phenol
Tetrahydrofuran
(THF)
Toluene
®
Waters PIC
Reagents
Solvent Considerations
C
99