Entering Parameter Values Into The Gradient Table - Waters 600e User Manual

Exiting the Program Gradient Screen
To exit the Program Gradient screen and enter another mode, press any function key
except the Program Method key.

10.1.3 Entering Parameter Values into the Gradient Table

To enter the parameter values into the table displayed on the Program Gradient screen:
1. Move the cursor to the Table # field.
2. Type a table number between 1 and 15. Press Enter.
If a gradient table is already stored under this number, that gradient table appears on
the screen. You can overwrite or edit this table, or enter another table number.
Note: The gradient table always runs with the event table of the same number.
3. If you are entering the initial conditions, continue with step 4 below.
Otherwise, type the elapsed time after injection (0.00 to 655.34 minutes) at which
the gradient segment is to start (or the previous segment is to end). Press Enter.
Each time entered into the table must be unique.
4. Move the cursor to the Flow field, type a flow-rate value between 0.00 and 20.0 or
45.00 mL/min. Press Enter.
5. Move the cursor to the %A field, type the appropriate percentage of the mobile
phase (0 to 100, in 1% increments, no decimals) for eluent reservoir A. Press Enter.
6. Repeat step 5 for the remaining three eluent reservoirs. The sum of the four eluent
percentages must equal 100 percent.
7. At the Curve field, type the gradient curve profile number for the gradient segment.
Figure 10-5 shows the gradient curve profiles.
Figure 10-5 Gradient Curve Profiles
Creating a Gradient Table 179