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Waters Manuals
Water Pump
1500-Series
Operator's manual
Waters 1500-Series Operator's Manual
Hplc pump
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Contents
Table of Contents
Troubleshooting
Bookmarks
Table of Contents
Copyright Notice
Trademarks
Customer Comments
Safety Considerations
Considerations Specific to the 1500-Series HPLC Pumps and Column Heater
Safety Advisories
Operating the Waters 1500-Series HPLC Pump and Options
Applicable Symbols
Audience and Purpose
Intended Use of the Waters 1500-Series HPLC Pump and Options
Calibrating
Quality-Control
ISM Classification
ISM Classification: ISM Group 1 Class B
EC Authorized Representative
Table of Contents
1 Introduction
HPLC Pump Operating Principles
Isocratic and Gradient LC System Operation
Effects of Dissolved Oxygen on the Mobile Phase
Using In-Line Degassing to Remove Gases from Eluents
Overview of the 1500-Series HPLC Pumps
Fluid-Handling Components
Electronic Components
Pump Control
Ethernet Configuration
IEEE-488 Configuration
Options and Accessories
1500-Series Column Heater
Integral Vacuum Degasser
Automated Plunger Seal Wash
Manual Injector
Ethernet Communications Kit
Gradient Mixers
2 Installing the HPLC Pump
Site Requirements
Unpacking
Connecting Power and Signal Cables
Connecting the Power Supply
Making Ethernet Connections
Making IEEE-488 Connections
Setting the IEEE-488 Address
Connecting Pump Inlet and Outlet Lines
Connecting the Eluent Supply
Connecting the Pump Outlet
Connecting Fluid Waste Lines
3 Installing Options and Accessories
Installing the 1500-Series Column Heater
Installing a Manual Injector
Connecting to the Column or Column Heater
Connecting the Inject Start Signal (for Manual Injector)
Installing Different Eluent Mixers
Installing the Integral Vacuum Degasser
Connecting Tubing to the Degasser Inlet and Outlet
Installing the Degasser Vent Line
Using the Degasser
Installing the Plunger Seal Wash System
Preparing the Instrument
Removing Pump Head Components
Installing Head Support Components (1515/1525 Pump Only)
Installing Head Support Components (1525EF Pump Only)
Installing Head Components (1525Μ Pump Only)
Installing the Solenoid
Installing Seal Wash Tubing
Using the Seal Wash System
4 Preparing for Operation
Startup and Initial Preparation
Powering-On the Pump
Pump Preparation Recommendations
Dry Priming the Pump
Operating with the Integral Vacuum Degasser
Operating with the Plunger Seal Wash System
Maximum Flow Rates for 1500-Series Pumps
Preparing for Breeze 2 Operation
Priming and Purging the Pump Via Breeze 2 Control
Purging the Flow Path Via Breeze 2 Control
Equilibrating the System Via Breeze 2 Control
Preparing for Empower 2 Operation
Priming and Purging the Pump Via Empower 2 Control
Purging the Flow Path Via Empower 2 Control
Equilibrating the System Via Empower 2 Control
Preparing for Masslynx Operation
Priming and Purging the Pump Via Masslynx Control
Purging the Flow Path Via Masslynx Control
Equilibrating the System Via Masslynx Control
Powering off the Pump
5 Maintaining the HPLC Pump
Maintenance Considerations
Safety and Handling
Proper Operating Procedures
Spare Parts
Contacting Waters Technical Service
Performing Pump Diagnostic Tests
Retention Time Stability Test
Ramp-And-Decay Test
Replacing and Cleaning Plunger Seals and Plungers
Preparing for Plunger Seal Replacement
Cleaning and Replacing the Plungers
Replacing Check Valves
Replacing 1515/1525 Check Valves
Replacing 1525EF Check Valves
Replacing 1525Μ Check Valves
Replacing a Draw-Off Valve
Removing the Draw-Off Valve
Installing a Draw-Off Valve
Replacing Fuses
Replacing the Rear Panel Fuses
6 Troubleshooting
Troubleshooting Pump Problems
Identifying and Correcting Noises
Identifying Chromatographic Problems
A Safety Advisories
Warning Symbols
Task-Specific Hazard Warnings
Specific Warnings
Caution Symbol
Warnings that Apply to All Waters Instruments
Electrical and Handling Symbols
Electrical Symbols
Handling Symbols
B Specifications
C Solvent Considerations
Introduction
Clean Solvents
Solvent Quality
Preparation Checklist
Water
Buffers
Tetrahydrofuran (THF
Solvent Compatibility
Solvents to Avoid
Solvents to Use
Solvent Miscibility
How to Use Miscibility Numbers (M-Numbers
Buffered Solvents
Head Height
Solvent Viscosity
Mobile Phase Solvent Degassing
Gas Solubility
Eluent Degassing Methods
Wavelength Selection
UV Cutoffs for Common Solvents
Mixed Mobile Phases
Refractive Indices of Common Solvents
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Overview of the 1500-Series Hplc Pumps
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Waters 1500-Series
HPLC Pump and Options
Operator's Guide
715002013IVD /Revision C
Copyright © Waters Corporation 2014
All rights reserved
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Table of Contents
Troubleshooting
Troubleshooting
135
Troubleshooting pump problems
136
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This manual is also suitable for:
1515
1525
1525u
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