Waters 600e User Manual page 197

• Sparge
• Temperature
Flow Rate
Specifies the eluent flow rate.
Note: Some columns require a flow ramp up. See the column Care and Use Manual for
guidelines.
Be sure that the values for the maximum and minimum operating pressures programmed
on the Pump Setup screen are appropriate for your column. See
Pump Setup Parameters
the Pump Setup Screen
Manual for pressure guidelines.
To set the Flow Rate:
1. Move the cursor to the Flow Rate field.
2. Type the appropriate eluent flow rate (0.00 to 20.0 or 45.00 mL/min) for the
application. Press Enter.
The system immediately sets the pump to the flow rate you entered.
Composition
Indicates the percentage (0 to 100%) of each eluent that will be used in the mobile phase.
To set the eluent composition:
1. Move the cursor to the New %A field.
Note: The Current values indicate the present eluent composition. The system
copies the values from the Current line when you move the cursor to the New line.
2. Type the % composition for the mobile phase (0 to 100, no decimals, in 1%
increments) for eluent reservoir A. Press Enter.
3. Repeat steps 1 and 2 for the remaining three eluent reservoirs. The sum of all four
entries must equal 100 percent.
4. After you enter the percentage for eluent D and press Enter, the system implements
all the eluent composition changes. The Current line reflects the new eluent
composition.
(for PowerLine Controller operation), or
(for Gradient Controller operation). See the column Care and Use
Section 4.3.2, Setting
Section 8.2.3, Exiting
Setting Run Conditions 169