Waters 600e User Manual page 190

Chart Out
Determines which signal is available at the Chart terminal on the rear panel of the
controller. This 0- to 10-mV signal can be the eluent flow rate (Flow) or the composition
percentage of any one of the four eluents (%A, %B, %C, or %D). To use this feature,
connect the Chart Out terminals on the rear panel of the controller to the 0- to 10-mV
terminal on the recorder or integrator.
This output signal can be connected to a data system or the second pen of a chart recorder.
It is frequently used during troubleshooting to reflect a selected condition (such as pump
flow or eluent composition).
A time delay between the eluent composition shown by the Chart Out signal and the eluent
composition at the column inlet always occurs. This time delay is the system volume or
delay volume. The 600E system has been optimized to reduce the delay volume.
The detector signal is further delayed by the volume at the column. At a low flow rate,
with a column attached, the delay between an eluent changeover instruction and its
subsequent detection may be several minutes.
To set the Chart Out parameter:
1. Move the cursor to the Chart Out field.
2. Type the number for the desired output:
• 1, 2, 3, or 4 for the % composition of one of the four eluents (A, B, C, or D)
• 5 for flow rate
• 6 for column heater temperature
3. Press Enter.
Flow Factor
Speeds up or slows down the pump to compensate for differences in pressure or eluent
compressibility. Enter a value here only if you need to match flow rate conditions between
systems or between different eluents.
To determine the flow factor for your application:
1. Set the flow rate to 1.0 mL/min.
2. Measure the actual flow rate.
162 Setting Gradient Controller Operating Parameters