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Flow
Specifies the eluent flow rate.
To set the Flow Rate:
1. Move the cursor to the Flow field if necessary.
2. Type the appropriate eluent flow rate (0.00 to 20.0 or 45.00 mL/min) for the
application and press Enter.
The system immediately sets the pump to the flow rate you entered.
Note: Some columns require a flow ramp-up. See the column Care and Use Manual for
guidelines.
%A,%B,%C,%D
Indicates the percentage (0 to 100%) of each eluent that will be used in the mobile phase.
To set the eluent composition:
1. Move the cursor to the New %A field.
Note: The Current values indicate how the pump is presently set to operate. The
system copies the values from the Current column when you move the cursor to the
New column.
2. Type the % composition (0 to 100, no decimals, in 1% increments) for eluent
reservoir A. Press Enter.
3. Repeat steps 1 and 2 for the three remaining eluent reservoirs. The sum of all four
entries must equal 100 percent.
After you enter the percentage for eluent D and press Enter, the system implements
the composition changes for all eluents. The Current column reflects the new eluent
composition.
Setting Run Conditions
89
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