Gradient Controller Configuration - Waters 600e User Manual

• Method table - A time-based program that contains up to 48 individual steps. Each
step accesses a set of gradient/event/detector tables. You can program the Method
table to automatically:
– Apply multiple separation methods to a sample set
– Equilibrate the system for a specified period of time
– Change over eluents
– Purge the autoinjector
– Purge the 410 detector flow cell
– Activate automatic system purge and shutdown
– Lower the system flow rate after an analysis
Once you have programmed the Method table, you may instruct the system to start
at a specific step number and vial number.
The PowerLine configuration also provides control for optional dissolution testing. For
details on dissolution control, see the Waters Dissolution System Operator's Manual.

2.4.2 Gradient Controller Configuration

When to Use Gradient Controller Mode
Use the Gradient Controller configuration to:
• Control the 600E system from an IEEE-based data system, such as the Waters
Empower or Millennium Chromatography Manager Data System.
• Operate the 600E system in stand-alone mode.
Description
The Waters 600E system in a Gradient Controller configuration:
• Controls isocratic separation conditions.
• Allows you to program and run gradient tables that define the varying eluent
composition, flow rate, time range, and rate of change for each phase of the
gradient.
• Allows you to program and run external event tables that control output signals to
external devices, internal alarms, sparge rate, column heater temperature, and
linking operation with other tables while running a gradient.
Note: The gradient controller does not control any external devices through the IEEE-488
or RS-232 connections.
24 Basic Operation