Preparing Your 600E System For Operation - Waters 600e User Manual

Chapter 3
Preparing Your 600E System
for Operation
This chapter contains procedures for preparing your Waters 600E Multisolvent Delivery
System for operation.
You perform some of these procedures only once (the first time you operate the system).
You must perform other procedures every time you use the system.
Preparing the System in PowerLine and Gradient Mode
This chapter assumes the 600E system is in PowerLine Controller mode when you prepare
the system. Each time you press the Direct key, the Direct Control screen appears (see
Figure 3-2).
You can perform the procedures listed in this chapter when the 600E system is in Gradient
Controller mode. However, when you press the Direct key, the Isocratic screen appears.
Set the parameters on the Isocratic screen as instructed in the procedures for the Direct
Control screen.
Good Operating Practices
To ensure proper operation of your system:
Caution: Always follow safe laboratory practices when handling eluents. Know the
physical and chemical properties of the eluents. See the Material Safety Data Sheets for
the eluents in use.
• Use only clean, HPLC-grade eluents. Filter all eluents. This is especially critical
with aqueous buffers.
• Never pump eluents that react with the material of the wetted surfaces in the fluid
system. See
• Avoid placing eluents on top of the system unless the bottles are in a container that
can hold the total volume of all of the eluents in case bottle leakage occurs.
Section Appendix B, Eluent Considerations, for more information.
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